Cap1 (NM_001301067) Mouse Tagged ORF Clone

CAT#: MR230088

Cap1 (myc-DDK-tagged) - Mouse CAP, adenylate cyclase-associated protein 1 (yeast) (Cap1), transcript variant 1

Product Images

Circular map for MR230088

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cap1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230088 representing NM_001301067
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGACATGCAAAATCTTGTAGAAAGATTGGAGAGGGCAGTGGGCCGCCTGGAGGCAGTGTCACATA
  CTTCAGACATGCACTGTGGATATGGAGACAGCCCTTCAAAAGGAGCAGTTCCATATGTGCAAGCATTTGA
  CTCGCTGCTTGCCAATCCCGTGGCAGAGTACTTGAAGATGAGTAAGGAGATCGGGGGAGATGTGCAGAAA
  CACGCGGAGATGGTCCACACAGGCCTGAAGTTGGAGCGAGCTCTCCTGGCTACAGCTTCTCAGTGCCAGC
  AGCCAGCTGGTAATAAACTTTCTGATTTGTTGGCACCTATCTCGGAGCAGATCCAAGAAGTTATAACCTT
  CCGGGAGAAGAACCGAGGCAGCAAGTTTTTTAATCATCTATCTGCTGTCAGTGAAAGCATCCAGGCCTTG
  GGCTGGGTGGCTCTGGCTGCGAAACCTGGCCCCTTTGTGAAAGAGATGAATGACGCGGCCATGTTTTACA
  CAAATCGTGTCCTCAAGGAGTACAGAGATGTGGATAAGAAGCATGTGGACTGGGTCAGAGCTTACTTGAG
  TATATGGACGGAGCTGCAGGCTTACATCAAGGAGTTTCATACTACTGGCCTGGCCTGGAGCAAGACGGGG
  CCTGTGGCAAAAGAACTGAGTGGATTGCCATCTGGACCCTCTGTGGGATCAGGCCCACCTCCTCCCCCAC
  CGGGCCCGCCTCCTCCCCCAATTCCTACCAGTTCTGGTTCTGACGACTCTGCATCACGCTCAGCACTGTT
  TGCACAGATTAATCAGGGGGAAAGCATCACACATGCCCTGAAACATGTATCTGATGACATGAAGACTCAC
  AAGAACCCTGCCCTGAAAGCTCAGAGCGGTCCAGTTCGGAGTGGCCCCAAACCATTCTCTGCACCTAAAC
  CCCAAACTAGCCCCTCCCCCAAACCAGCCACAAAGAAGGAACCAGCTCTGCTGGAACTGGAAGGCAAGAA
  ATGGAGAGTGGAAAACCAGGAGAATGTTTCTAACCTGGTGATTGATGACACTGAGCTGAAGCAGGTGGCT
  TACATCTACAAGTGTGTCAACACAACATTGCAAATCAAGGGCAAAATTAACTCCATTACAGTAGATAACT
  GTAAGAAGCTTGGCCTGGTGTTTGATGACGTGGTGGGCATTGTGGAGATAATCAATAGTAGGGATGTCAA
  AGTTCAGGTGATGGGAAAAGTGCCAACCATTTCCATTAACAAAACAGATGGCTGCCATGCTTACCTGAGC
  AAGAACTCCCTGGACTGTGAGATAGTCAGTGCCAAATCTTCTGAGATGAATGTCCTCATTCCTACCGAAG
  GCGGTGATTTTAACGAGTTCCCAGTCCCCGAGCAGTTCAAGACCCTGTGGAACGGACAGAAGTTGGTCAC
  CACAGTGACAGAAATCGCTGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230088 representing NM_001301067
  Red=Cloning site Green=Tags(s)
  
  MADMQNLVERLERAVGRLEAVSHTSDMHCGYGDSPSKGAVPYVQAFDSLLANPVAEYLKMSKEIGGDVQK
  HAEMVHTGLKLERALLATASQCQQPAGNKLSDLLAPISEQIQEVITFREKNRGSKFFNHLSAVSESIQAL
  GWVALAAKPGPFVKEMNDAAMFYTNRVLKEYRDVDKKHVDWVRAYLSIWTELQAYIKEFHTTGLAWSKTG
  PVAKELSGLPSGPSVGSGPPPPPPGPPPPPIPTSSGSDDSASRSALFAQINQGESITHALKHVSDDMKTH
  KNPALKAQSGPVRSGPKPFSAPKPQTSPSPKPATKKEPALLELEGKKWRVENQENVSNLVIDDTELKQVA
  YIYKCVNTTLQIKGKINSITVDNCKKLGLVFDDVVGIVEIINSRDVKVQVMGKVPTISINKTDGCHAYLS
  KNSLDCEIVSAKSSEMNVLIPTEGGDFNEFPVPEQFKTLWNGQKLVTTVTEIAG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301067
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301067.1, NP_001287996.1
• RefSeq Size: 2765 bp
• RefSeq ORF: 1425 bp
• Locus ID: 12331
• UniProt ID: P40124
• Cytogenetics: 4 D2.2
• MW: 51.6 kDa
• Gene Summary: The product of this gene plays a role in regulating actin dynamics by binding actin monomers and promoting the turnover of actin filaments. Reduced expression of this gene causes a reduction in actin filament turnover rates, causing multiple defects, including an increase in cell size, stress-fiber alterations, and defects in endocytosis and cell motility. A pseudogene of this gene is found on chromosome 14. Alternative splicing results in multiple transcript variants, but does not affect the protein. [provided by RefSeq, Jul 2014]