Rora (NM_001289916) Mouse Tagged ORF Clone

CAT#: MR230057

Rora (myc-DDK-tagged) - Mouse RAR-related orphan receptor alpha (Rora), transcript variant 2

Product Images

Circular map for MR230057

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rora
• Synonyms: 9530021D13Rik; nmf267; Nr1f1; ROR1; ROR2; ROR3; sg; staggerer; tmgc26
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230057 representing NM_001289916
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTTTGTGATCGCAGCGATGAAAGCTCAAATTGAAATTATTCCATGCAAGATCTGTGGAGACAAAT
  CGTCAGGAATCCATTATGGTGTCATTACGTGTGAAGGCTGCAAGGGCTTTTTCAGGAGAAGTCAGCAGAG
  CAATGCCACCTACTCCTGTCCTCGTCAGAAGAACTGTTTGATTGATCGGACCAGCAGAAACCGCTGCCAG
  CATTGTCGGCTGCAGAAATGCCTGGCCGTGGGGATGTCTCGAGATGCTGTCAAGTTTGGTCGGATGTCCA
  AGAAGCAGAGAGACAGCTTGTACGCCGAGGTGCAGAAGCACCGGATGCAGCAGCAGCAGCGAGACCACCA
  GCAGCAGCCTGGGGAGGCGGAGCCGCTGACGCCCACCTACAACATCTCAGCCAATGGGCTGACGGAACTG
  CATGATGACCTCAGCACCTATATGGATGGGCACACCCCCGAGGGCAGCAAGGCCGACTCAGCCGTCAGCA
  GCTTCTACCTGGACATCCAGCCCTCCCCAGACCAGTCGGGATTGGACATCAATGGGATCAAACCCGAACC
  CATATGTGACTACACACCAGCATCTGGCTTCTTCCCCTACTGTTCCTTCACCAACGGAGAGACTTCCCCA
  ACCGTGTCCATGGCAGAACTAGAACACCTTGCCCAGAACATATCCAAATCCCACCTGGAAACCTGCCAGT
  ACTTGCGGGAAGAGCTCCAGCAGATAACGTGGCAGACCTTCCTGCAGGAGGAGATTGAAAACTACCAGAA
  CAAGCAGAGAGAGGTGATGTGGCAGCTGTGTGCCATCAAGATTACAGAAGCTATCCAGTATGTGGTGGAG
  TTTGCCAAACGCATTGATGGATTTATGGAGCTGTGTCAAAATGATCAAATTGTGCTTCTAAAAGCAGGCT
  CGCTAGAGGTGGTGTTTATTAGGATGTGCCGTGCCTTTGACTCTCAGAACAACACCGTGTACTTTGACGG
  GAAGTATGCGAGCCCCGATGTCTTCAAGTCCCTAGGTTGTGAAGACTTCATCAGCTTTGTGTTTGAATTT
  GGGAAGAGTTTGTGTTCTATGCACCTGACCGAAGACGAAATCGCGTTATTTTCTGCATTCGTACTGATGT
  CAGCGGATCGCTCGTGGCTTCAGGAAAAGGTAAAAATAGAAAAGCTGCAACAGAAAATTCAGCTGGCCCT
  TCAGCACGTCCTACAGAAGAACCACCGAGAAGATGGAATTCTAACCAAGCTAATATGCAAGGTGTCTACG
  TTAAGAGCCCTATGTGGACGACATACGGAAAAGCTAATGGCATTTAAAGCAATATACCCAGACATTGTGC
  GACTCCATTTTCCTCCATTATACAAGGAATTGTTCACTTCAGAATTTGAGCCAGCCATGCAAATCGATGG
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230057 representing NM_001289916
  Red=Cloning site Green=Tags(s)
  
  MYFVIAAMKAQIEIIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQSNATYSCPRQKNCLIDRTSRNRCQ
  HCRLQKCLAVGMSRDAVKFGRMSKKQRDSLYAEVQKHRMQQQQRDHQQQPGEAEPLTPTYNISANGLTEL
  HDDLSTYMDGHTPEGSKADSAVSSFYLDIQPSPDQSGLDINGIKPEPICDYTPASGFFPYCSFTNGETSP
  TVSMAELEHLAQNISKSHLETCQYLREELQQITWQTFLQEEIENYQNKQREVMWQLCAIKITEAIQYVVE
  FAKRIDGFMELCQNDQIVLLKAGSLEVVFIRMCRAFDSQNNTVYFDGKYASPDVFKSLGCEDFISFVFEF
  GKSLCSMHLTEDEIALFSAFVLMSADRSWLQEKVKIEKLQQKIQLALQHVLQKNHREDGILTKLICKVST
  LRALCGRHTEKLMAFKAIYPDIVRLHFPPLYKELFTSEFEPAMQIDG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289916
• ORF Size: 1401 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289916.1, NP_001276845.1
• RefSeq Size: 10869 bp
• RefSeq ORF: 1404 bp
• Locus ID: 19883
• UniProt ID: P51448
• Cytogenetics: 9 37.45 cM
• MW: 53.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]