Matk (NM_001285855) Mouse Tagged ORF Clone

CAT#: MR230051

Matk (myc-DDK-tagged) - Mouse megakaryocyte-associated tyrosine kinase (Matk), transcript variant 4

Product Images

Circular map for MR230051

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Matk
• Synonyms: CHK; HYL; Ntk; p56ntk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230051 representing NM_001285855
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAACGCGCTGGGCCCCTGGGACTCAATGCATGACCAAGTGTGAGAACTCTCGCCCCAAGCCCGGTG
  AGCTAGCCTTTCGAAAGGGTGACATGGTGACCATCTTGGAGGCCTGTGAGGACAAGAGCTGGTACCGAGC
  CAAGCACCATGGCAGTGGGCAGGAAGGGCTGCTGGCGGCCGCTGCTCTGCGACAGCGGGAGGCCCTCTCC
  ACAGACCCCAAGCTCAGCCTCATGCCATGGTTTCATGGCAAGATCTCCGGCCAGGAAGCCATACAGCAGC
  TGCAGCCACCCGAGGACGGGCTGTTCCTTGTGAGGGAATCAGCTCGTCACCCTGGAGACTATGTCTTGTG
  TGTCAGTTTCGGCCGTGACGTCATCCACTACCGTGTTTTGCATCGAGATGGGCACCTCACCATCGATGAG
  GCCGTGTGTTTCTGTAACCTGATGGACATGGTGGAGCACTACACCAAGGACAAGGGGGCCATCTGCACCA
  AGCTGGTGAAGCCAAGGAGGAAACAGGGCGCAAAGTCTGCAGAGGAGGAGCTCGCCAAGGCTGGCTGGCT
  ACTCGACCTGCAGCATCTGACTCTGGGAGCACAGATTGGAGAGGGGGAGTTTGGAGCCGTCCTACAGGGT
  GAGTACCTGGGACAGAAGGTGGCTGTGAAGAATATCAAGTGTGATGTGACAGCCCAGGCCTTCCTGGATG
  AGACGGCTGTGATGACGAAGCTGCAGCACAGGAACCTAGTGCGACTCCTGGGTGTGATCCTGCACCACGG
  CTTGTACATTGTCATGGAGCACGTGAGCAAGGGCAACCTGGTGAACTTCCTGCGCACGCGGGGCCGTGCT
  CTTGTGAGCACCTCTCAGCTTCTGCAGTTTGCTCTTCATGTTGCTGAAGGCATGGAATACCTGGAGAGCA
  AGAAGCTGGTGCACCGGGACCTGGCTGCTCGGAACATCCTGGTCTCTGAGGACTTGGTGGCCAAGGTCAG
  TGACTTTGGCTTAGCCAAGGCAGAGCGCAAGGGGCTGGACTCAAGCCGGCTGCCAGTCAAGTGGACGGCA
  CCTGAGGCTCTCAAAAACGGGCGGTTCTCCAGCAAGTCGGATGTCTGGAGTTTTGGGGTGCTGTTGTGGG
  AAGTCTTCTCTTATGGAAGAGCCCCATACCCCAAGATGTCGCTAAAGGAGGTTTCAGAGGCTGTGGAGAA
  GGGTTACCGCATGGAGCCCCCCGATGGCTGCCCAGGCTCTGTGCACACCCTCATGGGTAGCTGCTGGGAG
  GCAGAGCCTGCGCGCCGACCACCCTTCCGCAAAATAGTGGAGAAGCTGGGCCGTGAGCTCCGCAGTGTGG
  GTGTCTCGGCCCCCGCTGGGGGACAGGAGGCTGAGGGCTCAGCTCCCACACGGAGCCAGGACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230051 representing NM_001285855
  Red=Cloning site Green=Tags(s)
  
  MPTRWAPGTQCMTKCENSRPKPGELAFRKGDMVTILEACEDKSWYRAKHHGSGQEGLLAAAALRQREALS
  TDPKLSLMPWFHGKISGQEAIQQLQPPEDGLFLVRESARHPGDYVLCVSFGRDVIHYRVLHRDGHLTIDE
  AVCFCNLMDMVEHYTKDKGAICTKLVKPRRKQGAKSAEEELAKAGWLLDLQHLTLGAQIGEGEFGAVLQG
  EYLGQKVAVKNIKCDVTAQAFLDETAVMTKLQHRNLVRLLGVILHHGLYIVMEHVSKGNLVNFLRTRGRA
  LVSTSQLLQFALHVAEGMEYLESKKLVHRDLAARNILVSEDLVAKVSDFGLAKAERKGLDSSRLPVKWTA
  PEALKNGRFSSKSDVWSFGVLLWEVFSYGRAPYPKMSLKEVSEAVEKGYRMEPPDGCPGSVHTLMGSCWE
  AEPARRPPFRKIVEKLGRELRSVGVSAPAGGQEAEGSAPTRSQDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001285855
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285855.1, NP_001272784.1
• RefSeq Size: 1912 bp
• RefSeq ORF: 1398 bp
• Locus ID: 17179
• UniProt ID: P41242
• Cytogenetics: 10 C1
• MW: 52 kDa
• Gene Summary: Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation.[UniProtKB/Swiss-Prot Function]