Tfap2b (NM_001286340) Mouse Tagged ORF Clone

CAT#: MR229985

Tfap2b (myc-DDK-tagged) - Mouse transcription factor AP-2 beta (Tfap2b), transcript variant 3

Product Images

Circular map for MR229985

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tfap2b
• Synonyms: AI606113; AP-2(beta); E130018K07Rik; Tcfap2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229985 representing NM_001286340
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTCACCTCCTAGAGACCAGGCTGCGATCATGCTCTGGAAACTCGTGGAGAATGTCAAGTACGAAG
  ACATCTATGAGGACCGGCACGATGGCGTCCCAAGCCATAGCTCGAGACTCTCTCAACTGGGCTCTGTGTC
  CCAAGGACCCTACTCAAGCGCCCCGCCGCTGTCCCACACGCCATCATCGGACTTCCAGCCACCCTACTTT
  CCGCCCCCCTACCAGCCGCTACCCTACCACCAGAGTCAAGACCCGTACTCCCACGTCAACGATCCCTACT
  CCCTGAACCCTCTGCATCAGCCCCAGCAGCACCCGTGGGGGCAACGGCAACGCCAAGAAGTGGGCTCAGA
  AGCCGGCTCTCTCCTGCCCCAGCCCCGGGCAGCCTTGCCCCAGCTCTCCGGCCTTGATCCCCGAAGGGAC
  TACCACTCTGTCCGCCGGCCGGACGTGCTGCTGCATTCCGCACATCACGGCCTGGACGCCGGCATGGGCG
  ACAGCCTCTCGTTGCACGGCCTTGGGCATCCCGGCATGGAAGACGTGCAGTCAGTTGAAGATGCCAATAA
  CAGCGGCATGAACCTATTGGACCAGTCAGTCATTAAAAAAGTTCCTGTCCCTCCCAAATCTGTGACTTCT
  CTAATGATGAATAAAGATGGCTTCTTGGGAGGAATGTCAGTCAACACCGGCGAGGTATTTTGCTCAGTCC
  CGGGCCGTTTATCTCTACTCAGTTCAACTTCAAAGTACAAAGTCACTGTGGGCGAAGTTCAGAGAAGGCT
  CTCGCCCCCTGAATGCCTCAATGCATCTCTCCTGGGCGGCGTCCTCAGAAGAGCCAAATCGAAAAATGGG
  GGGAGATCCTTGAGAGAAAGGCTAGAAAAAATCGGTTTGAATTTACCCGCGGGCAGGCGCAAAGCAGCAA
  ATGTCACGTTACTCACCTCACTGGTAGAAGGGGAAGCTGTTCACTTAGCTCGGGATTTCGGGTATATTTG
  TGAAACAGAGTTTCCTGCTAAAGCCGTGTCCGAGTATTTGAACAGACAGCACACGGACCCCAGTGACCTG
  CACTCCAGAAAGAATATGCTGCTGGCCACCAAGCAACTTTGTAAAGAATTTACGGATCTGCTGGCTCAGG
  ACCGGACACCGATCGGAAACAGCAGGCCCAGCCCCATCCTGGAGCCGGGCATCCAAAGCTGTCTCACGCA
  CTTCAGTCTCATCACGCACGGCTTCGGTGCCCCGGCCATTTGCGCTGCGCTCACGGCCCTGCAGAACTAT
  CTCACCGAGGCGCTCAAAGGCATGGACAAGATGTTCTTGAACAACACCACTAACAGGCACACGTCTGGGG
  AAGGCCCAGGGCAACGCGCCCCACGCGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229985 representing NM_001286340
  Red=Cloning site Green=Tags(s)
  
  MHSPPRDQAAIMLWKLVENVKYEDIYEDRHDGVPSHSSRLSQLGSVSQGPYSSAPPLSHTPSSDFQPPYF
  PPPYQPLPYHQSQDPYSHVNDPYSLNPLHQPQQHPWGQRQRQEVGSEAGSLLPQPRAALPQLSGLDPRRD
  YHSVRRPDVLLHSAHHGLDAGMGDSLSLHGLGHPGMEDVQSVEDANNSGMNLLDQSVIKKVPVPPKSVTS
  LMMNKDGFLGGMSVNTGEVFCSVPGRLSLLSSTSKYKVTVGEVQRRLSPPECLNASLLGGVLRRAKSKNG
  GRSLRERLEKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYICETEFPAKAVSEYLNRQHTDPSDL
  HSRKNMLLATKQLCKEFTDLLAQDRTPIGNSRPSPILEPGIQSCLTHFSLITHGFGAPAICAALTALQNY
  LTEALKGMDKMFLNNTTNRHTSGEGPGQRAPRV
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286340
• ORF Size: 1359 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286340.1, NP_001273269.1
• RefSeq Size: 3831 bp
• RefSeq ORF: 1362 bp
• Locus ID: 21419
• Cytogenetics: 1 A3
• MW: 50.1 kDa
• Gene Summary: Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.[UniProtKB/Swiss-Prot Function]