Tfap2b (NM_001286340) Mouse Untagged Clone

CAT#: MC227774

Tfap2b (untagged) - Mouse transcription factor AP-2 beta (Tfap2b), transcript variant 3


  "NM_001286340" in other vectors (1)

Reconstitution Protocol

USD 503.00

2 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Tfap2b
Synonyms AI606113; AP-2(beta); E130018K07Rik; Tcfap2b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227774 representing NM_001286340
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACTCACCTCCTAGAGACCAGGCTGCGATCATGCTCTGGAAACTCGTGGAGAATGTCAAGTACGAAG
ACATCTATGAGGACCGGCACGATGGCGTCCCAAGCCATAGCTCGAGACTCTCTCAACTGGGCTCTGTGTC
CCAAGGACCCTACTCAAGCGCCCCGCCGCTGTCCCACACGCCATCATCGGACTTCCAGCCACCCTACTTT
CCGCCCCCCTACCAGCCGCTACCCTACCACCAGAGTCAAGACCCGTACTCCCACGTCAACGATCCCTACT
CCCTGAACCCTCTGCATCAGCCCCAGCAGCACCCGTGGGGGCAACGGCAACGCCAAGAAGTGGGCTCAGA
AGCCGGCTCTCTCCTGCCCCAGCCCCGGGCAGCCTTGCCCCAGCTCTCCGGCCTTGATCCCCGAAGGGAC
TACCACTCTGTCCGCCGGCCGGACGTGCTGCTGCATTCCGCACATCACGGCCTGGACGCCGGCATGGGCG
ACAGCCTCTCGTTGCACGGCCTTGGGCATCCCGGCATGGAAGACGTGCAGTCAGTTGAAGATGCCAATAA
CAGCGGCATGAACCTATTGGACCAGTCAGTCATTAAAAAAGTTCCTGTCCCTCCCAAATCTGTGACTTCT
CTAATGATGAATAAAGATGGCTTCTTGGGAGGAATGTCAGTCAACACCGGCGAGGTATTTTGCTCAGTCC
CGGGCCGTTTATCTCTACTCAGTTCAACTTCAAAGTACAAAGTCACTGTGGGCGAAGTTCAGAGAAGGCT
CTCGCCCCCTGAATGCCTCAATGCATCTCTCCTGGGCGGCGTCCTCAGAAGAGCCAAATCGAAAAATGGG
GGGAGATCCTTGAGAGAAAGGCTAGAAAAAATCGGTTTGAATTTACCCGCGGGCAGGCGCAAAGCAGCAA
ATGTCACGTTACTCACCTCACTGGTAGAAGGGGAAGCTGTTCACTTAGCTCGGGATTTCGGGTATATTTG
TGAAACAGAGTTTCCTGCTAAAGCCGTGTCCGAGTATTTGAACAGACAGCACACGGACCCCAGTGACCTG
CACTCCAGAAAGAATATGCTGCTGGCCACCAAGCAACTTTGTAAAGAATTTACGGATCTGCTGGCTCAGG
ACCGGACACCGATCGGAAACAGCAGGCCCAGCCCCATCCTGGAGCCGGGCATCCAAAGCTGTCTCACGCA
CTTCAGTCTCATCACGCACGGCTTCGGTGCCCCGGCCATTTGCGCTGCGCTCACGGCCCTGCAGAACTAT
CTCACCGAGGCGCTCAAAGGCATGGACAAGATGTTCTTGAACAACACCACTAACAGGCACACGTCTGGGG
AAGGCCCAGGGCAACGCGCCCCACGCGTGTGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_001286340
Insert Size 1362 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001286340.1, NP_001273269.1
RefSeq Size 3831 bp
RefSeq ORF 1362 bp
Locus ID 21419
Cytogenetics 1 A3
Gene Summary Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.