Dach2 (NM_001289734) Mouse Tagged ORF Clone

CAT#: MR229965

Dach2 (myc-DDK-tagged) - Mouse dachshund 2 (Drosophila) (Dach2), transcript variant 5

Product Images

Circular map for MR229965

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dach2
• Synonyms: 9430028N04Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229965 representing NM_001289734
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGGCCATGAAGCTCCAGAAGATGAAGCTTATGGCAATGAATACTCTTCAGGGTAATGGAAGCC
  AAAATGGGACTGAGTCAGAGCCAGATGACCTTAATTCTACGACAGGTGGAAGTGAATCCTCCTGGGATAA
  AGATAAGATACAATCTCCACTTGCTGCTTCTGGACCTCAACATGGAATTGCTCATGCAGCACTTGCTGGT
  CAGCCAGGCCTTGGAGGTGCTCCGACCCTTAATCCACTTCAGCAGAATCACCTGCTAAGCAATCGTCTGG
  ATCTCCCATTTATGATGATGCCTCATCCCCTACTTCCAGTCAGCTTACCTCCTGCATCAGTTGCCATGGC
  AATGAATCAGATGAATCATCTCAATACTATTGCCAACATGGCTGCTGCAGCCCAGATTCACAGTCCACTC
  TCCAGAGCTGGTGCCTCTGTTATAAAGGAACGGATCCCAGAGAGTCCTTCTCCTGCTCCCTCTCTGGAAG
  AGAGTCATCGTCCTGGGAGCCAGACCTCCTCCCACCCAAGCAGCAGTGTGTCCAGCTCTCCCTCGCAGAT
  GGATCATCATTCAGAGAGAATGGTTATGATGCCCAACAATCGAGAAGAGCTTATTGTTGACCAAGATAAT
  GGACAAAGCATAAAAAAATTCCAGAGGGATAATAAAGATATCCAATTGTCCCAGCACCATTTGTTGAACA
  CTTTTCTTCACTGGATTGAGTTGGCACCATTATCAAAAAAATCACTTCACCATCAAAAAGAAGTACCAGC
  TCAAATCCCAGTCATGAAGTCACCCTTGGATAAGATCCAGTTGGCTCCTGGACAGGCATTGCATCCTGGA
  TTCCCTGGACCATTCATTTTTGCAGATAGTCTATCTTCTGTGGAGACTCTGTTGACCAACATTCAGGTCA
  ACAATATTTCTATAAACAAAATAAATGGTCTACTGAAAGTGGCTTTGGATAATGCTCGTATCCAGGAGAA
  GCAGATTCAGCAGGAAAAGAAGGAACTGCGAATAGAGCTCTTCAGAGAAAGAGAAATTAGAGAAAACCTG
  GAGCGACAACTTGCAGTTGAGCTTCAAAGCAGAAGTACAATGCAAAAGCGTCTGAAAAAAGAGAAAAAGG
  CTAAGAGAAAACTTCAGGAAGCCTTGGAATTTGAATCAAAGCGCCGCGAGCAAGTGGAGCAAGCACTTAA
  ACAAGCTACATCTGGTGACAGTGGACTGAGAATGTTAAAAGATTCTGGAATTCCAGATATTGAAATAGAA
  AACAGTGGAACGCCGCATGACAGTGCTGCTATGCAAGGAGGTAACTATTACTGTTTAGCAATGGCACAGC
  AGTTGTGTTCAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229965 representing NM_001289734
  Red=Cloning site Green=Tags(s)
  
  MAEAMKLQKMKLMAMNTLQGNGSQNGTESEPDDLNSTTGGSESSWDKDKIQSPLAASGPQHGIAHAALAG
  QPGLGGAPTLNPLQQNHLLSNRLDLPFMMMPHPLLPVSLPPASVAMAMNQMNHLNTIANMAAAAQIHSPL
  SRAGASVIKERIPESPSPAPSLEESHRPGSQTSSHPSSSVSSSPSQMDHHSERMVMMPNNREELIVDQDN
  GQSIKKFQRDNKDIQLSQHHLLNTFLHWIELAPLSKKSLHHQKEVPAQIPVMKSPLDKIQLAPGQALHPG
  FPGPFIFADSLSSVETLLTNIQVNNISINKINGLLKVALDNARIQEKQIQQEKKELRIELFREREIRENL
  ERQLAVELQSRSTMQKRLKKEKKAKRKLQEALEFESKRREQVEQALKQATSGDSGLRMLKDSGIPDIEIE
  NSGTPHDSAAMQGGNYYCLAMAQQLCSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289734
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289734.1, NP_001276663.1
• RefSeq Size: 2963 bp
• RefSeq ORF: 1347 bp
• Locus ID: 93837
• UniProt ID: Q925Q8
• Cytogenetics: X 49.13 cM
• MW: 49.8 kDa
• Gene Summary: Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression. Probably binds to DNA via its DACHbox-N domain.[UniProtKB/Swiss-Prot Function]