Dach2 (NM_001289734) Mouse Untagged Clone

CAT#: MC227754

Dach2 (untagged) - Mouse dachshund 2 (Drosophila) (Dach2), transcript variant 5


  "NM_001289734" in other vectors (1)

Reconstitution Protocol

USD 503.00

2 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Dach2
Synonyms 9430028N04Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227754 representing NM_001289734
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAGGCCATGAAGCTCCAGAAGATGAAGCTTATGGCAATGAATACTCTTCAGGGTAATGGAAGCC
AAAATGGGACTGAGTCAGAGCCAGATGACCTTAATTCTACGACAGGTGGAAGTGAATCCTCCTGGGATAA
AGATAAGATACAATCTCCACTTGCTGCTTCTGGACCTCAACATGGAATTGCTCATGCAGCACTTGCTGGT
CAGCCAGGCCTTGGAGGTGCTCCGACCCTTAATCCACTTCAGCAGAATCACCTGCTAAGCAATCGTCTGG
ATCTCCCATTTATGATGATGCCTCATCCCCTACTTCCAGTCAGCTTACCTCCTGCATCAGTTGCCATGGC
AATGAATCAGATGAATCATCTCAATACTATTGCCAACATGGCTGCTGCAGCCCAGATTCACAGTCCACTC
TCCAGAGCTGGTGCCTCTGTTATAAAGGAACGGATCCCAGAGAGTCCTTCTCCTGCTCCCTCTCTGGAAG
AGAGTCATCGTCCTGGGAGCCAGACCTCCTCCCACCCAAGCAGCAGTGTGTCCAGCTCTCCCTCGCAGAT
GGATCATCATTCAGAGAGAATGGTTATGATGCCCAACAATCGAGAAGAGCTTATTGTTGACCAAGATAAT
GGACAAAGCATAAAAAAATTCCAGAGGGATAATAAAGATATCCAATTGTCCCAGCACCATTTGTTGAACA
CTTTTCTTCACTGGATTGAGTTGGCACCATTATCAAAAAAATCACTTCACCATCAAAAAGAAGTACCAGC
TCAAATCCCAGTCATGAAGTCACCCTTGGATAAGATCCAGTTGGCTCCTGGACAGGCATTGCATCCTGGA
TTCCCTGGACCATTCATTTTTGCAGATAGTCTATCTTCTGTGGAGACTCTGTTGACCAACATTCAGGTCA
ACAATATTTCTATAAACAAAATAAATGGTCTACTGAAAGTGGCTTTGGATAATGCTCGTATCCAGGAGAA
GCAGATTCAGCAGGAAAAGAAGGAACTGCGAATAGAGCTCTTCAGAGAAAGAGAAATTAGAGAAAACCTG
GAGCGACAACTTGCAGTTGAGCTTCAAAGCAGAAGTACAATGCAAAAGCGTCTGAAAAAAGAGAAAAAGG
CTAAGAGAAAACTTCAGGAAGCCTTGGAATTTGAATCAAAGCGCCGCGAGCAAGTGGAGCAAGCACTTAA
ACAAGCTACATCTGGTGACAGTGGACTGAGAATGTTAAAAGATTCTGGAATTCCAGATATTGAAATAGAA
AACAGTGGAACGCCGCATGACAGTGCTGCTATGCAAGGAGGTAACTATTACTGTTTAGCAATGGCACAGC
AGTTGTGTTCAGCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001289734
Insert Size 1347 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001289734.1, NP_001276663.1
RefSeq Size 2963 bp
RefSeq ORF 1347 bp
Locus ID 93837
UniProt ID Q925Q8
Cytogenetics X 49.13 cM
Gene Summary Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression. Probably binds to DNA via its DACHbox-N domain.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (5) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at an alternate start codon, compared to variant 1. The encoded protein (isoform 5) is shorter and has a distinct C-terminus, compared to isoform 1.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.