Kcnj16 (NM_001252210) Mouse Tagged ORF Clone

CAT#: MR229810

Kcnj16 (myc-DDK-tagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 16 (Kcnj16), transcript variant 5

Product Images

Circular map for MR229810

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnj16
• Synonyms: 6430410F18Rik; AI132396; Kir5.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229810 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTATTACGGAAGTAGCTACAGGATCGTCAATGTGGACTCCAAATATCCAGGCTATCCTCCAGAGC
  ATGCCATCGCTGAGAAGAGAAGAGCAAGAAGGCGCTTGCTCCACAAAGATGGCAGCTGTAATGTGTACTT
  TAAACACATTTTTGGAGAATGGGGGAGCTACATGGTTGATATTTTTACCACTCTTGTGGATACCAAGTGG
  CGCCATATGTTCGTAATATTTTCTCTGTCTTACATTCTCTCCTGGTTGATATTTGGCTCCATATTTTGGC
  TCATAGCCTTTCATCACGGAGACCTATTAAGCGATCCAGATATCACCCCTTGTGTTGACAACGTGCATTC
  ATTTACGGCTGCATTTTTATTCTCCCTGGAGACCCAGACCACCATTGGATACGGTTACCGCTGTGTCACC
  GAAGAGTGCTCTGTGGCTGTACTGACAGTGATCCTTCAGTCCATCCTCAGCTGCATCATAAACACCTTCA
  TCATTGGAGCAGCCTTGGCAAAGATGGCAACTGCCCGGAAGAGAGCCCAGACCATACGCTTCAGCTATTT
  TGCCCTCATTGGTATGAGAGACGGGAAGCTTTGCCTCATGTGGCGCATAGGTGACTTCCGACCAAACCAT
  GTGGTAGAGGGCACGGTGAGAGCCCAACTTCTGCGCTATTCAGAAGACAGTGAAGGGAGGATGACGATGG
  CGTTTAAAGACCTCAAACTCGTCAATGACCAGATAATCCTGGTAACTCCAGTGACTATTGTCCATGAAAT
  TGACCATGAGAGCCCTCTGTATGCCCTTGACCGCAAGGCAGTGGCCAAAGATAATTTCGAGATTCTGGTG
  ACATTTATTTATACTGGTGATTCCACTGGGACATCCCACCAGTCCAGAAGTTCCTACATCCCCAGAGAAA
  TTCTCTGGGGCCACAGGTTTCATGATGTATTGGAAGTGAAGAGAAAGTACTACAAGGTGAACTGCTTGCA
  GTTTGAAGGAAGCGTGGAAGTCTACGCCCCCTTTTGCAGTGCCAAACAACTGGACTGGAAGGACCAACAA
  CTCAACAACTTGGAGAAAACGTCCCCTGCCCGAGGATCCTGCAATTCTGACACCAACACCAGGAGGCGGT
  CCTTCAGCGCAGTTGCCGTGGTGAGCAGCTGTGAGAACCCAGAGGAGACCGTCCTGTCCCCACAAGATGA
  ATGTAAGGAGATGCCCTATCAGAAAGCCCTCCTGACTTTAAATAGGATCTCCATGGAATCCCAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229810 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSYYGSSYRIVNVDSKYPGYPPEHAIAEKRRARRRLLHKDGSCNVYFKHIFGEWGSYMVDIFTTLVDTKW
  RHMFVIFSLSYILSWLIFGSIFWLIAFHHGDLLSDPDITPCVDNVHSFTAAFLFSLETQTTIGYGYRCVT
  EECSVAVLTVILQSILSCIINTFIIGAALAKMATARKRAQTIRFSYFALIGMRDGKLCLMWRIGDFRPNH
  VVEGTVRAQLLRYSEDSEGRMTMAFKDLKLVNDQIILVTPVTIVHEIDHESPLYALDRKAVAKDNFEILV
  TFIYTGDSTGTSHQSRSSYIPREILWGHRFHDVLEVKRKYYKVNCLQFEGSVEVYAPFCSAKQLDWKDQQ
  LNNLEKTSPARGSCNSDTNTRRRSFSAVAVVSSCENPEETVLSPQDECKEMPYQKALLTLNRISMESQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252210
• ORF Size: 1257 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252210.2, NP_001239139.1
• RefSeq Size: 3652 bp
• RefSeq ORF: 1260 bp
• Locus ID: 16517
• UniProt ID: Q9Z307
• Cytogenetics: 11 75.01 cM
• MW: 48 kDa
• Gene Summary: Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ16 may be involved in the regulation of fluid and pH balance. In the kidney, together with KCNJ10, mediates basolateral K(+) recycling in distal tubules; this process is critical for Na(+) reabsorption at the tubules.[UniProtKB/Swiss-Prot Function]