Celf3 (NM_001289616) Mouse Tagged ORF Clone

CAT#: MR229655

Celf3 (myc-DDK-tagged) - Mouse CUGBP, Elav-like family member 3 (Celf3), transcript variant 3

Product Images

Circular map for MR229655

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Celf3
• Synonyms: 4930415M08Rik; BRUNOL1; CAGH4; ERDA4; Tnrc4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229655 representing NM_001289616
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAGGCCGATCCAGGTCAAGCCAGCCGACAGCGAGAGTCGAGGAGACCGGAAGCTCTTTGTGGGCA
  TGCTAGGAAAGCAGCAGACAGATGAGGATGTCCGGAAGATGTTTGAACCATTTGGGACTATAGACGAGTG
  CACTGTGCTCCGGGGGCCAGACGGTACCAGCAAAGGCTGTGCCTTTGTGAAGTTCCAGACTCACGCTGAG
  GCCCAGGCAGCCATCAACACCCTTCACAGCAGCCGGACCCTACCGGGTGCCTCATCCAGCCTGGTGGTAA
  AGTTTGCTGACACGGAGAAGGAGCGAGGTCTCCGTCGAATGCAGCAGGTGGCTACCCAGCTGGGCATGTT
  CAGCCCGATCGCCCTCCAGTTTGGAGCCTACAGCGCCTACACCCAGGCCCTGATGCAGCAGCAGGCGGCC
  CTGGTAGCAGCTCACAGTGCCTACCTCAGCCCTATGGCCACCATGGCTGCCGTGCAGATGCAGCACATGG
  CTGCCATCAGTGCCAATGGCCTCATCGCCACCCCCATCACTCCATCCTCAGGAACCAGCACCCCTCCTGC
  CATTGCTGCCACGCCCGTCTCTGCCATCCCTGCTGCCTTGGGCGTCAACGGCTACAGCCCGGTGCCCACC
  CAGCCTACAGGGCAGCCTGCCCCGGATGCTCTGTATCCCAACGGGGTTCACCCTTACCCAGCCCAGAGCC
  CGGCAGCCCCCGTGGACCCTCTCCAGCAGGCCTATGCAGGAATGCAGCACTACACAGCAGCGTACCCCGC
  AGCCTACAGCCTGGTTGCACCTGCGTTCCCGCAGCCTCCAGCCCTGGTTGCCCAGCAGCCCCCACCACCA
  CCTCAGCAACAGCAGCAGCAGCAGCAGCAGCAACAGCAACAGCAGCAGCAACGGGAAGGCCCTGATGGCT
  GCAACATCTTCATCTACCACCTGCCCCAGGAGTTCACAGACTCAGAGATCCTCCAGATGTTTGTCCCTTT
  TGGTCATGTCATCTCAGCCAAAGTCTTTGTTGACCGGGCCACCAATCAGAGCAAATGTTTTGGCTTTGTG
  AGTTTCGACAATCCGGCCAGTGCCCAGGCTGCCATCCAGGCTATGAACGGTTTCCAGATTGGCATGAAGC
  GCCTCAAAGTCCAGCTAAAGCGGCCTAAGGATGCAAACAGGCCCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229655 representing NM_001289616
  Red=Cloning site Green=Tags(s)
  
  MNRPIQVKPADSESRGDRKLFVGMLGKQQTDEDVRKMFEPFGTIDECTVLRGPDGTSKGCAFVKFQTHAE
  AQAAINTLHSSRTLPGASSSLVVKFADTEKERGLRRMQQVATQLGMFSPIALQFGAYSAYTQALMQQQAA
  LVAAHSAYLSPMATMAAVQMQHMAAISANGLIATPITPSSGTSTPPAIAATPVSAIPAALGVNGYSPVPT
  QPTGQPAPDALYPNGVHPYPAQSPAAPVDPLQQAYAGMQHYTAAYPAAYSLVAPAFPQPPALVAQQPPPP
  PQQQQQQQQQQQQQQQQREGPDGCNIFIYHLPQEFTDSEILQMFVPFGHVISAKVFVDRATNQSKCFGFV
  SFDNPASAQAAIQAMNGFQIGMKRLKVQLKRPKDANRPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289616
• ORF Size: 1167 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289616.1, NP_001276545.1
• RefSeq Size: 2534 bp
• RefSeq ORF: 1170 bp
• Locus ID: 78784
• UniProt ID: Q8CIN6
• Cytogenetics: 3 F2.1
• MW: 42.3 kDa
• Gene Summary: RNA-binding protein involved in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity).[UniProtKB/Swiss-Prot Function]