Btrc (NM_001286466) Mouse Tagged ORF Clone

CAT#: MR229625

Btrc (myc-DDK-tagged) - Mouse beta-transducin repeat containing protein (Btrc), transcript variant 4

Product Images

Circular map for MR229625

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Btrc
• Synonyms: b-TrCP; beta-TrCP; Beta-Trcp1; E3RS-IkappaB; E3RSIkappaB; Fbw1a; FWD1; HOS; Slimb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229625 representing NM_001286466
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGGAAAAAGCTCATCGAGAGGATGGTCAGGACGGACTCTCTGTGGCGAGGCCTGGCAGAGCGCA
  GAGGCTGGGGACAGTACTTATTCAAAAACAAACCTCCTGATGAGAACGCTCCTCCCAACTCCTTTTATAG
  AGCGCTTTATCCTAAAATCATACAAGACATTGAGACAATAGAGTCCAATTGGAGATGTGGGCGACATAGT
  TTACAGAGAATCCACTGCCGGAGTGAAACAAGTAAAGGGGTTTACTGTTTACAGTACGACGACCAGAAGA
  TAGTCAGCGGCCTTCGAGACAACACCATCAAGATCTGGGATAAAAGCACACTGGAATGCAAGCGGATTCT
  CACGGGCCACACGGGCTCCGTCCTGTGTCTGCAGTACGATGAGAGGGTGATCATCACAGGCTCCTCAGAC
  TCCACCGTCAGAGTGTGGGATGTAAATGCAGGTGAGATGCTAAACACATTGATTCACCACTGTGAAGCCG
  TTCTGCACCTGCGCTTCAATAATGGCATGATGGTGACCTGTTCCAAAGACCGTTCCATCGCTGTGTGGGA
  TATGGCTTCCCCAACTGACATCACCCTCAGGAGGGTGCTGGTGGGACACCGAGCTGCGGTCAATGTTGTA
  GACTTTGATGACAAGTACATCGTTTCTGCCTCTGGAGATAGAACCATAAAGGTGTGGAACACAAGTACCT
  GTGAATTCGTAAGGACCCTAAATGGGCACAAGCGTGGCATCGCCTGTTTGCAGTACAGAGACAGGCTGGT
  GGTGAGCGGCTCCTCTGACAACACCATCAGGCTGTGGGACATAGAGTGTGGAGCATGCCTGCGAGTGTTG
  GAGGGCCATGAGGAGTTGGTACGCTGCATTCGATTTGATAACAAAAGGATAGTGAGCGGAGCCTATGATG
  GGAAAATTAAAGTGTGGGATCTTATGGCTGCTTTGGACCCGCGTGCTCCAGCAGGGACTCTCTGTCTGCG
  GACACTTGTGGAGCATTCTGGAAGAGTTTTCCGCCTCCAGTTTGATGAATTCCAGATTGTCAGTAGTTCA
  CATGATGACACAATTCTCATCTGGGACTTCCTGAATGATCCAGCTGCTCACGCTGAACCGCCCCGCTCCC
  CTTCTCGGACATACACCTACATCTCCAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229625 representing NM_001286466
  Red=Cloning site Green=Tags(s)
  
  MLWKKLIERMVRTDSLWRGLAERRGWGQYLFKNKPPDENAPPNSFYRALYPKIIQDIETIESNWRCGRHS
  LQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKSTLECKRILTGHTGSVLCLQYDERVIITGSSD
  STVRVWDVNAGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVV
  DFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVL
  EGHEELVRCIRFDNKRIVSGAYDGKIKVWDLMAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSS
  HDDTILIWDFLNDPAAHAEPPRSPSRTYTYISR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286466
• ORF Size: 1149 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286466.1, NP_001273395.1
• RefSeq Size: 6126 bp
• RefSeq ORF: 1152 bp
• Locus ID: 12234
• UniProt ID: Q3ULA2
• Cytogenetics: 19 C3
• MW: 44.4 kDa
• Gene Summary: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins (PubMed:10097128, PubMed:16371461, PubMed:18782782, PubMed:9859996, PubMed:9990853, PubMed:21911472). SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB, ATF4, CDC25A, DLG1, FBXO5, PER1, SMAD3, SMAD4, SNAI1 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling (By similarity). SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription (PubMed:9859996, PubMed:10097128). Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22' (PubMed:9859996, PubMed:10097128). SCF(BTRC) mediates the ubiquitination of CEP68; this is required for centriole separation during mitosis (By similarity). SCF(BTRC) mediates the ubiquitination and subsequent degradation of nuclear NFE2L1 (PubMed:21911472). Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and PER2 (PubMed:18782782). May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase (By similarity). Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF (By similarity). Required for proteolytic processing of GLI3 (PubMed:16371461). Mediates ubiquitination of REST, thereby leading to its proteasomal degradation (By similarity).[UniProtKB/Swiss-Prot Function]