P2ry2 (NM_001302347) Mouse Tagged ORF Clone

CAT#: MR229557

P2ry2 (myc-DDK-tagged) - Mouse purinergic receptor P2Y, G-protein coupled 2 (P2ry2), transcript variant 3

Product Images

Circular map for MR229557

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: P2ry2
• Synonyms: P2U1; P2Y2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229557 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCAGACCTGGAACCCTGGAATAGCACCATCAATGGCACCTGGGAGGGGGACGAACTGGGATACA
  AGTGTCGTTTCAACGAGGACTTCAAGTACGTGCTGTTGCCCGTGTCCTATGGCGTGGTGTGCGTGCTCGG
  GTTGTGCCTGAACGTCGTGGCTCTCTATATCTTCCTATGCCGCCTCAAAACCTGGAACGCCTCCACCACC
  TACATGTTTCACCTGGCAGTTTCGGACTCTCTCTACGCAGCGTCCCTGCCGCTGTTGGTTTATTACTACG
  CCCGGGGTGACCACTGGCCATTTAGCACGGTGCTCTGCAAGCTGGTGCGTTTCCTCTTCTACACCAACCT
  CTACTGCAGCATCCTCTTCCTCACCTGCATCAGCGTGCACCGGTGCCTGGGAGTCCTGCGCCCTCTGCAC
  TCCCTGCGTTGGGGCCGGGCCCGTTATGCCCGCCGGGTGGCTGCGGTTGTGTGGGTGCTGGTGCTGGCCT
  GCCAGGCACCCGTGCTCTACTTCGTCACCACCAGCGTGCGGGGAACCCGGATCACTTGCCATGACACCTC
  GGCCCGAGAGCTCTTTAGCCATTTTGTGGCTTACAGCTCCGTCATGCTGGGTCTGCTTTTTGCTGTGCCC
  TTTTCCGTAATCCTGGTCTGTTACGTGCTTATGGCCAGGCGGCTGCTCAAACCGGCTTATGGGACCACAG
  GAGGTCTGCCTCGGGCCAAGCGCAAGTCTGTGCGCACCATTGCCTTGGTACTGGCCGTCTTCGCCCTCTG
  CTTCCTGCCTTTCCACCTCACGCGCACCCTCTACTACTCCTTCCGATCACTTGACCTCAGCTGCCACACC
  CTCAACGCCATCAACATGGCATATAAGATCACCCGGCCGCTGGCCAGCGCCAACAGTTGTCTTGACCCGG
  TACTCTACTTCCTGGCAGGGCAGAGACTTGTCCGCTTTGCCCGAGATGCCAAGCCACCCACGGAGCCTAC
  CCCCAGCCCACAGGCTCGTCGCAAGCTGGGCCTGCACAGGCCTAACAGAACTGTGAGGAAAGATTTGTCA
  GTCAGCAGTGACGACTCAAGACGGACAGAGTCCACACCAGCTGGAAGTGAGACTAAGGACATTCGGCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229557 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAADLEPWNSTINGTWEGDELGYKCRFNEDFKYVLLPVSYGVVCVLGLCLNVVALYIFLCRLKTWNASTT
  YMFHLAVSDSLYAASLPLLVYYYARGDHWPFSTVLCKLVRFLFYTNLYCSILFLTCISVHRCLGVLRPLH
  SLRWGRARYARRVAAVVWVLVLACQAPVLYFVTTSVRGTRITCHDTSARELFSHFVAYSSVMLGLLFAVP
  FSVILVCYVLMARRLLKPAYGTTGGLPRAKRKSVRTIALVLAVFALCFLPFHLTRTLYYSFRSLDLSCHT
  LNAINMAYKITRPLASANSCLDPVLYFLAGQRLVRFARDAKPPTEPTPSPQARRKLGLHRPNRTVRKDLS
  VSSDDSRRTESTPAGSETKDIRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302347
• ORF Size: 1119 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302347.1, NP_001289276.1
• RefSeq Size: 2820 bp
• RefSeq ORF: 1122 bp
• Locus ID: 18442
• UniProt ID: P35383
• Cytogenetics: 7 E2
• MW: 42.2 kDa
• Gene Summary: Receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. The affinity range is UTP = ATP > ATP-gamma-S >> 2-methylthio-ATP = ADP.[UniProtKB/Swiss-Prot Function]