Pdlim2 (NM_001253736) Mouse Tagged ORF Clone

CAT#: MR229410

Pdlim2 (myc-DDK-tagged) - Mouse PDZ and LIM domain 2 (Pdlim2), transcript variant 2

Product Images

Circular map for MR229410

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pdlim2
• Synonyms: 4732462F18Rik; Slim
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229410 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTTGACTGTGGATGTGGCAGGACCAGCACCTTGGGGCTTCCGAATTAGCGGGGGCAGAGATTTCC
  ACACACCCATCATTGTGACCAAGGTCACAGAGCGGGGCAAGGCTGAAGCAGCTGATCTCCGGCCTGGCGA
  CATCATTGTGGCCATCAATGGACAGAGTGCAGAGAACATGCTACACGCGGAGGCCCAAAGCAAGATCCGA
  CAGAGCGCCTCACCCCTAAGACTGCAGCTGGACCGGTCCCAAACAGCCTCTCCTGGGCAGACCAATGGGG
  AGGGCTCCTTGGAAGTGCTGGCAACCAGATTCCAGGGCTCCCTGAGGACACACCGTGACAGCCAGTCTTC
  CCAGAGGTCTGCCTGCTTCAGCCCAGTCTCTCTCAGCCCCAGGCCTTGCAGCCCCTTCTCCACCCCACCC
  CCTACCAGCCCAGTTGCCCTTTCTAAAGAGGATATGATTGGCTGTAGTTTCCAGAGTCTGACACACTCTC
  CAGGCCTTGCTGCTGCTCACCACTTGACCTACCCTGGCCACCCCACCAGCCAACAGGCCGGCCACAGCAG
  CCCAAGCGACTCCGCAGTGAGGGTGCTGCTCCATTCCCCAGGACGGCCCTCCAGCCCTAGGTTCAGCAGT
  TTGGATCTGGAGGAAGACTCAGAGGTGTTCAAGATGCTGCAGGAGAACCGCCAGGGACGGGCCGCCCCAA
  GGCAGTCCAGCTCTTTTCGACTCTTACAGGAAGCCTTGGAGGCTGAGGAGAGAGGTGGCACACCTGCCTT
  TGTGCCCAGCTCGCTGAGCTCCCAGGCTTCCTTGCCCACCTCCAGGGCCTTGGCCACTCCACCCAAGCTC
  CACACCTGTGAGAAATGCAGCGTCAACATCTCGAACCAGGCGGTCCGCATCCAGGAGGGGAGGTACCGAC
  ACCCTGGCTGCTACACTTGCGCAGACTGTGGGCTGAACCTGAAGATGCGCGGCCACTTCTGGGTGGGCAA
  TGAGTTGTACTGCGAGAAGCATGCCCGCCAGCGCTACTCTATGCCTGGAACTCTCAACTCTCGAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229410 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALTVDVAGPAPWGFRISGGRDFHTPIIVTKVTERGKAEAADLRPGDIIVAINGQSAENMLHAEAQSKIR
  QSASPLRLQLDRSQTASPGQTNGEGSLEVLATRFQGSLRTHRDSQSSQRSACFSPVSLSPRPCSPFSTPP
  PTSPVALSKEDMIGCSFQSLTHSPGLAAAHHLTYPGHPTSQQAGHSSPSDSAVRVLLHSPGRPSSPRFSS
  LDLEEDSEVFKMLQENRQGRAAPRQSSSFRLLQEALEAEERGGTPAFVPSSLSSQASLPTSRALATPPKL
  HTCEKCSVNISNQAVRIQEGRYRHPGCYTCADCGLNLKMRGHFWVGNELYCEKHARQRYSMPGTLNSRA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001253736
• ORF Size: 1050 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253736.1, NP_001240665.1
• RefSeq Size: 1587 bp
• RefSeq ORF: 1050 bp
• Locus ID: 213019
• UniProt ID: Q8R1G6
• Cytogenetics: 14 D2
• MW: 37.7 kDa
• Gene Summary: Probable adapter protein located at the actin cytoskeleton that promotes cell attachment. Necessary for the migratory capacity of epithelial cells. Overexpression enhances cell adhesion to collagen and fibronectin and suppresses anchorage independent growth. May contribute to tumor cell migratory capacity (By similarity).[UniProtKB/Swiss-Prot Function]