Cyb5r2 (NM_001205227) Mouse Tagged ORF Clone

CAT#: MR229055

Cyb5r2 (myc-DDK-tagged) - Mouse cytochrome b5 reductase 2 (Cyb5r2), transcript variant 1

Product Images

Circular map for MR229055

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyb5r2
• Synonyms: b5R.2; D630003K02Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229055 representing NM_001205227
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGTCAAGAAGAAAGATCTTATCACCTTACAGGACCCTGAAGCCAAGTACCCGCTGCCCTTGATTG
  AGAAGGAGCAAATCAGTCACAACACCAGAAGGTTCCGCTTTGGGCTGCCCTCACCAGACCATGTCCTGGG
  GCTTCCTGTCGGTAACTATGTCCATCTCTTGGCACAAATTAACAATGAATTGGTGATCAGGGCTTACACA
  CCTGTCTCCAGTGACGATGATCAAGGCTTTGTGGACTTAATTATAAAGATCTACTTCAAAAATGTGCACC
  CCAAGTATCCTGAAGGAGGAAAGATGACTCAGTACTTGGAGAACATGAAAATTGGGGATACCATCCTTTT
  TCGGGGGCCAACAGGGCGCCTGTTCTACAATGAGCCAGGCTGCCAGACAAGGGCTGCAGAAGTCAAAAAC
  ATCTTTATATTTCTAGGAACCCTTTTAATCAAAGCAAACAAAACAAGTGAGCCTGAAAAAAAGCTGGTTC
  ACCACCTGGGGATGATTGCTGGAGGCACAGGCATTACGCCCATGCTACAGCTGATTCGCCACATCACCAA
  GGACACCAGCGATGAGACCAGGATGTCCCTCCTCTTTGCTAACCAGACAGAAGAGGATATCTTGCTGAGA
  AAGGAGCTAGAAGAAGTCGCCACGACTCATCACAAACAGTTCAACCTGTGGTATACCCTGGACAGGCCTC
  CCAGTGACTGGAAGTACAGCTCAGGCTTTGTTAGCGCTGACATGATCAAGGAGCACCTCCCTCCTCCTGG
  GGAGGACACGCTCATCCTGGTGTGTGGCCCACCACCTCTGATCCAGGCGGCAGCTCACCCCAGCCTGGAG
  CAGCTCAGCTATACCAAGGACATGATTTTTATCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229055 representing NM_001205227
  Red=Cloning site Green=Tags(s)
  
  MSVKKKDLITLQDPEAKYPLPLIEKEQISHNTRRFRFGLPSPDHVLGLPVGNYVHLLAQINNELVIRAYT
  PVSSDDDQGFVDLIIKIYFKNVHPKYPEGGKMTQYLENMKIGDTILFRGPTGRLFYNEPGCQTRAAEVKN
  IFIFLGTLLIKANKTSEPEKKLVHHLGMIAGGTGITPMLQLIRHITKDTSDETRMSLLFANQTEEDILLR
  KELEEVATTHHKQFNLWYTLDRPPSDWKYSSGFVSADMIKEHLPPPGEDTLILVCGPPPLIQAAAHPSLE
  QLSYTKDMIFIY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001205227
• ORF Size: 876 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205227.1, NP_001192156.1
• RefSeq Size: 2772 bp
• RefSeq ORF: 879 bp
• Locus ID: 320635
• UniProt ID: Q3KNK3
• Cytogenetics: 7 E3
• MW: 33.6 kDa
• Gene Summary: NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1) (By similarity).[UniProtKB/Swiss-Prot Function]