Psme2b (NM_001281472) Mouse Tagged ORF Clone

CAT#: MR228712

Psme2b (myc-DDK-tagged) - Mouse protease (prosome, macropain) activator subunit 2B (Psme2b)

Product Images

Circular map for MR228712

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Psme2b
• Synonyms: PA28; PA28b-p; PA28b2; Psme; Psme2; Psme2-; Psme2-like; Psme2b-ps
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228712 representing NM_001281472
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGCCTTGTGGGGTCCGCTTGAGTGGAGAAGCCCGAAAACAGGTGGATGTCTTCAGACAAAATC
  TTTTCCAGGAGGCTGATGACTTCCTCTGCACTTTCTTGCCACGGAAAATCATATCCCTGAGTCAGCTCTT
  GCAGGAGGATTCCCTCAATGTGGCCGACCTCTCCTCCCTCCGGGCTCCTCTGGACATCCCTATCCCAGAT
  CCTCCACCCAAGGATGACGAGATGGAAACAGACAAGCAGGAGAAGAAGGAAGTCCCTAAGTGTGGCTACC
  TCCCGGGGAACGAGAAGCTTCTGGCCTTGCTTGCTTTGGTTAAGCCAGAAGTCTGGACTCTCAAAGAGAA
  GTGCATTCTGGTAATCACGTGGATCCAGCACCTGATCCCCAAGATTGAGGATGGAAATGATTTTGGGGTG
  GCAATTCAGGAGAAGGTGCTGGAGAGAGTGAATGCTGTCAAGACCAAAGTAGAAGCTTTCCAGACAACTA
  TTTCCAAGTACTTCTCAGAACGAGGGGATGCTGTAGCCAAGGCTTCCAAGGATACCCATGTAATGGATTA
  CCGGGCCTTGGTGCATGAGCGAGATGAAGCAGCCTATGGGGCGCTTAGGGCCATGGTGCTGGACCTGAGG
  GCCTTCTATGCTGAGCTTTATCATATCATCAGCAGCAACCTAGAGAAAATCGTCAACCCGAAGGGTGAAG
  AAAAGCCATCGATGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228712 representing NM_001281472
  Red=Cloning site Green=Tags(s)
  
  MAKPCGVRLSGEARKQVDVFRQNLFQEADDFLCTFLPRKIISLSQLLQEDSLNVADLSSLRAPLDIPIPD
  PPPKDDEMETDKQEKKEVPKCGYLPGNEKLLALLALVKPEVWTLKEKCILVITWIQHLIPKIEDGNDFGV
  AIQEKVLERVNAVKTKVEAFQTTISKYFSERGDAVAKASKDTHVMDYRALVHERDEAAYGALRAMVLDLR
  AFYAELYHIISSNLEKIVNPKGEEKPSMY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001281472
• ORF Size: 717 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281472.1, NP_001268401.1
• RefSeq Size: 1066 bp
• RefSeq ORF: 720 bp
• Locus ID: 621823
• UniProt ID: P97372
• Cytogenetics: 11 B1.2
• MW: 27.5 kDa
• Gene Summary: This gene may encode proteasome activator complex subunit 2 protein (PMID: 10222192). This gene, located on chromosome 11, lacks a structured promoter and is intronless. It has also been described as a pseudogene (PMID: 9914329), however, it has an intact coding region and transcripts can be placed at this location unambiguously. An interferon-inducible gene located on chromosome 14, near the gene that encodes proteasome activator complex subunit 1 protein, is considered the functional gene. [provided by RefSeq, Jul 2013]