Mea1 (NM_001277311) Mouse Tagged ORF Clone

CAT#: MR228281

Mea1 (myc-DDK-tagged) - Mouse male enhanced antigen 1 (Mea1), transcript variant 4

Product Images

Circular map for MR228281

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mea1
• Synonyms: Mea-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR228281 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCAGTAGTTCTAGGGGGAGACACCATGGGCCCTGAGCGTATCTTCCCCAATCAGACTGAGGACT
  TAGGGCCACATCAGGGTCCTACAGAAGGAACTGGGGATTGGAGCAGTGAAGAACCCGAGGAAGAGCAGGA
  GGAAACAGGAGCAGGCCCAGCAGGCTACTCGTACCAGCCCCTCAATCAAGACCCTGAACAAGAAGAGGTG
  GAATTGGCACCAGTGGGTGAAGGAGAAGATGGAGCTGCTGACATCCAAGATCGGATCCAGGCCCTGGGGC
  TTCATCTGCCAGACCCTCCATTGGAGAGTGAGGATGAAGATGAGGAGGGAGCCGCCGCATTGAGCAGCCA
  CAGCTCTATCCCCATGGACCCAGAACATGTAGAGCTGGTGAAAAGGACGATGGCTGGAGTAAGCCTGCCT
  GCACCAGGGGTTCCTGCCTGGGCTCGGGAGATCTCGGACGCTCAGTGGGAAGATGTGGTACAGAAAGCCC
  TCCAAGCCCGGCAGGCATCCCCTGCCTGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR228281 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAVVLGGDTMGPERIFPNQTEDLGPHQGPTEGTGDWSSEEPEEEQEETGAGPAGYSYQPLNQDPEQEEV
  ELAPVGEGEDGAADIQDRIQALGLHLPDPPLESEDEDEEGAAALSSHSSIPMDPEHVELVKRTMAGVSLP
  APGVPAWAREISDAQWEDVVQKALQARQASPAWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277311
• ORF Size: 522 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277311.1, NP_001264240.1
• RefSeq Size: 858 bp
• RefSeq ORF: 525 bp
• Locus ID: 17256
• UniProt ID: Q64327
• Cytogenetics: 17 22.9 cM
• MW: 18.6 kDa
• Gene Summary: May play an important role in spermatogenesis and/or testis development.[UniProtKB/Swiss-Prot Function]