Tmigd3 (NM_027025) Mouse Tagged ORF Clone

CAT#: MR227580

Adora3 (Myc-DDK-tagged) - Mouse adenosine A3 receptor (Adora3), transcript variant 2

Product Images

Circular map for MR227580

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmigd3
• Synonyms: 1700001d09rik; 4930578J19Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227580 representing NM_027025
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTCCTCCTCCTTCTCTCCTTGGCTCTCTTTTCCGATGCCATGGTCATGGATGAAAAGGTGAAAT
  CAGGTGTTGAGCTGGAGACAGCTTCTGCAGTCTGTGTCTATGATGCCTACTACAAGGACCACACCAAGTA
  CTGGTGCCGTGGCTACTTCAGGGACTCGTGCAACATTATTGCCTTCACCCCTAATAGCACCAACCGTGTG
  GCCCTGAAGGATACAGGAAACCAACTCATTATCACTATATCCTGCCTGGTCAAGGAGGACACAGGCTGGT
  ACTGGTGTGGTATCCAGCGGGACTTGGCCAGAGATGACATGGATTTTACACAGCTGATTGTGACTGACAA
  TAGAGAAGACCGGGCCAATGGCTTTTCATCTGACCCTTCAGGCAATAGGACCCGGAGCTGTAGGGCTTCC
  AAAGCTGTCCAAAAGGCAGAGGGCTCCCGAATGTCCATTCTGATCATTTGTATACTGATTACCAGTTTGG
  GGATCATCTTTATAATCAGTCACTTGTCCAGAGGAAGGCGAAGCCAAAGAAATAGAGAAGTTACTGGCAA
  AAGCATCAGTAGAAACCCACAAGCAAGCCAAGGCCCGTCTATGGTCTCCATAACTTTGGCAAGGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227580 representing NM_027025
  Red=Cloning site Green=Tags(s)
  
  MEFLLLLSLALFSDAMVMDEKVKSGVELETASAVCVYDAYYKDHTKYWCRGYFRDSCNIIAFTPNSTNRV
  ALKDTGNQLIITISCLVKEDTGWYWCGIQRDLARDDMDFTQLIVTDNREDRANGFSSDPSGNRTRSCRAS
  KAVQKAEGSRMSILIICILITSLGIIFIISHLSRGRRSQRNREVTGKSISRNPQASQGPSMVSITLARI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027025
• ORF Size: 627 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027025.4, NP_081301.2
• RefSeq Size: 927 bp
• RefSeq ORF: 630 bp
• Locus ID: 69296
• UniProt ID: G3X8R9
• Cytogenetics: 3
• MW: 23.9 kDa
• Gene Summary: This gene encodes a transmembrane and immunoglobulin domain-containing protein. Alternative splicing results in multiple transcript variants, one of which shares its 3' terminal exon with that of the overlapping adenosine A3 receptor gene (GeneID:11542). [provided by RefSeq, Nov 2014]