Mybpc3 (NM_008653) Mouse Tagged ORF Clone

CAT#: MR227579

Mybpc3 (Myc-DDK-tagged) - Mouse myosin binding protein C, cardiac (Mybpc3)

Product Images

Circular map for MR227579

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mybpc3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227579 representing NM_008653
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGGTGTGACTGTTCTCAAGATGCCGGAGCCAGGGAAGAAACCAGTGTCAGCCTTCAACAAGAAGC
  CAAGGTCAGCGGAGGTGACCGCTGGCAGTGCTGCCGTGTTCGAGGCTGAGACGGAGCGGTCAGGCGTGAA
  GGTGCGGTGGCAGCGGGATGGCAGCGACATCACCGCCAATGACAAGTATGGTTTGGCAGCAGAGGGCAAG
  CGACACACACTGACAGTGCGGGATGCGAGCCCTGATGACCAGGGTTCCTACGCGGTCATTGCAGGCTCCT
  CAAAGGTCAAGTTTGACCTCAAGGTCACAGAGCCAGCCCCTCCAGAGAAGGCAGAATCTGAAGTTGCTCC
  AGGAGCCCCCAAAGAAGTCCCTGCTCCAGCCACTGAGTTGGAAGAAAGTGTCTCAAGTCCTGAAGGGTCA
  GTCTCGGTAACCCAGGATGGCTCAGCTGCAGAGCATCAGGGAGCCCCTGATGACCCTATTGGCCTCTTTC
  TGATGCGACCACAGGATGGTGAGGTGACCGTGGGCGGCAGCATTGTCTTCTCAGCCCGAGTGGCTGGGGC
  CAGCCTCCTGAAACCGCCTGTGGTCAAGTGGTTCAAGGGCAAGTGGGTGGACCTGAGCAGCAAAGTGGGC
  CAGCACCTGCAGCTGCATGACAGCTATGACAGAGCCAGCAAGGTCTACTTGTTTGAGTTGCACATCACAG
  ATGCTCAGACCACTTCTGCTGGGGGCTACCGCTGTGAGGTGTCTACCAAGGACAAATTTGACAGCTGTAA
  CTTCAACCTCACTGTCCATGAGGCCATTGGTTCTGGAGACCTGGACCTCAGATCAGCTTTCCGACGCACG
  AGCCTGGCGGGAGCAGGTCGGAGAACCAGTGACAGCCATGAAGATGCTGGGACTCTGGACTTTAGTTCCC
  TGCTGAAGAAGAGAGACAGTTTCCGGAGGGACTCAAAGCTGGAGGCACCTGCTGAAGAAGACGTGTGGGA
  GATCCTGAGACAGGCACCGCCGTCAGAATATGAGCGCATCGCCTTCCAGCACGGAGTCACAGACCTTCGA
  GGCATGCTGAAGAGGCTCAAGGGCATGAAGCAGGATGAAAAGAAGAGCACAGCCTTTCAGAAGAAGCTGG
  AGCCTGCCTACCAGGTAAACAAGGGCCACAAGATTCGGCTTACTGTGGAACTGGCTGATCCGGACGCCGA
  AGTCAAGTGGCTTAAGAATGGACAGGAGATCCAGATGAGTGGCAGCAAGTACATCTTCGAGTCCGTCGGT
  GCCAAGCGCACCCTGACCATCAGCCAGTGCTCACTGGCTGACGACGCAGCCTACCAGTGTGTGGTGGGGG
  GCGAGAAGTGCAGCACGGAGCTCTTTGTCAAAGAGCCCCCGGTGCTGATCACTCGGTCCCTGGAAGACCA
  GCTGGTGATGGTGGGTCAGCGGGTGGAGTTTGAGTGTGAGGTCTCAGAAGAAGGGGCCCAAGTCAAATGG
  CTGAAGGATGGGGTTGAGCTGACACGTGAGGAGACCTTCAAATACCGGTTCAAGAAAGATGGGCGGAAAC
  ACCACTTGATCATCAATGAAGCAACCCTGGAGGATGCAGGACACTATGCAGTACGCACAAGTGGAGGCCA
  GTCACTGGCTGAGCTCATTGTGCAAGAGAAGAAGTTGGAGGTATACCAAAGCATCGCGGACCTGGCAGTG
  GGAGCCAAGGACCAGGCTGTGTTTAAGTGTGAGGTTTCAGATGAGAATGTACGCGGCGTGTGGCTGAAGA
  ATGGGAAGGAACTGGTGCCTGACAACCGCATAAAGGTGTCCCATATAGGCCGGGTCCACAAACTGACCAT
  TGACGATGTCACACCTGCTGATGAGGCTGACTACAGCTTTGTCCCTGAAGGGTTTGCCTGCAACCTGTCT
  GCCAAGCTCCACTTCATGGAGGTCAAGATTGACTTTGTGCCTAGGCAGGAACCTCCCAAGATCCACTTGG
  ATTGTCCCGGCAGCACACCAGACACCATTGTGGTTGTTGCTGGGAACAAGTTACGCCTGGATGTCCCTAT
  TTCTGGAGACCCTGCTCCCACTGTGGTCTGGCAGAAGACTGTAACACAGGGGAAGAAGGCCTCAACTGGG
  CCACACCCTGATGCCCCAGAAGATGCTGGTGCTGATGAGGAGTGGGTGTTTGATAAGAAGCTGTTGTGTG
  AGACTGAGGGCCGGGTCCGGGTGGAGACCACCAAAGACCGCAGCGTCTTTACAGTCGAAGGGGCAGAGAA
  GGAAGATGAAGGTGTCTACACAGTCACAGTAAAGAACCCCGTGGGCGAGGACCAGGTCAACCTCACAGTC
  AAGGTCATCGATGTCCCAGATGCTCCTGCGGCCCCTAAGATCAGCAACGTGGGCGAGGACTCCTGCACTG
  TGCAGTGGGAACCGCCTGCCTATGATGGCGGGCAGCCGGTCCTGGGATACATCCTGGAGCGCAAGAAGAA
  AAAGAGCTACAGGTGGATGAGGCTCAACTTTGATCTGCTGCGGGAGCTGAGCCACGAGGCGAGGCGCATG
  ATCGAGGGTGTAGCCTATGAGATGCGAGTCTACGCAGTCAATGCCGTGGGAATGTCCAGGCCCAGCCCTG
  CCTCTCAGCCCTTCATGCCTATTGGGCCCCCTGGCGAACCAACCCACTTGGCTGTGGAGGATGTGTCAGA
  CACCACTGTCTCACTCAAGTGGCGGCCCCCAGAGCGCGTGGGGGCCGGTGGCCTGGACGGATACAGCGTG
  GAGTACTGCCAGGAGGGATGCTCCGAGTGGACACCTGCTCTGCAGGGGCTGACAGAGCGCACATCGATGC
  TGGTGAAGGACCTACCCACTGGGGCACGGCTGCTGTTCCGAGTACGGGCACACAATGTGGCAGGTCCTGG
  AGGCCCTATCGTCACCAAGGAGCCTGTGACAGTGCAGGAGATACTGCAACGACCACGGCTCCAACTGCCC
  AGACACCTGCGCCAGACCATCCAGAAGAAAGTTGGGGAGCCTGTGAACCTCCTCATCCCTTTCCAGGGCA
  AACCCCGGCCTCAGGTGACCTGGACCAAAGAGGGGCAGCCCCTGGCAGGTGAGGAGGTGAGCATCCGGAA
  CAGCCCCACAGACACGATCTTGTTCATCCGAGCTGCCCGCCGCACCCACTCGGGCACCTACCAGGTGACA
  GTTCGCATTGAGAACATGGAGGACAAGGCAACGCTGATCCTGCAGATTGTGGACAAGCCAAGTCCTCCCC
  AGGATATCCGGATCGTTGAGACTTGGGGTTTCAATGTGGCTCTGGAGTGGAAGCCACCCCAAGATGATGG
  CAATACAGAGATCTGGGGTTATACTGTACAGAAAGCTGACAAGAAGACCATGGAGTGGTTCACGGTTTTG
  GAACACTACCGACGCACTCACTGTGTGGTATCAGAGCTTATCATTGGCAATGGCTACTACTTCCGGGTCT
  TCAGCCATAACATGGTGGGTTCCAGTGACAAAGCTGCCGCCACCAAGGAGCCAGTCTTTATTCCAAGACC
  AGGCATCACATATGAGCCACCCAAATACAAGGCCCTGGACTTCTCTGAGGCCCCAAGCTTCACCCAGCCC
  TTGGCAAATCGCTCCATCATTGCAGGCTATAATGCCATCCTCTGCTGTGCTGTCCGAGGTAGTCCTAAGC
  CCAAGATTTCCTGGTTCAAGAATGGCCTGGATCTGGGAGAAGATGCTCGCTTCCGCATGTTCTGCAAGCA
  GGGAGTATTGACCCTGGAGATCAGGAAACCCTGCCCCTATGATGGTGGTGTCTATGTCTGCAGGGCCACC
  AACTTGCAGGGCGAGGCACAGTGTGAGTGCCGCCTGGAGGTGCGAGTTCCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227579 representing NM_008653
  Red=Cloning site Green=Tags(s)
  
  MPGVTVLKMPEPGKKPVSAFNKKPRSAEVTAGSAAVFEAETERSGVKVRWQRDGSDITANDKYGLAAEGK
  RHTLTVRDASPDDQGSYAVIAGSSKVKFDLKVTEPAPPEKAESEVAPGAPKEVPAPATELEESVSSPEGS
  VSVTQDGSAAEHQGAPDDPIGLFLMRPQDGEVTVGGSIVFSARVAGASLLKPPVVKWFKGKWVDLSSKVG
  QHLQLHDSYDRASKVYLFELHITDAQTTSAGGYRCEVSTKDKFDSCNFNLTVHEAIGSGDLDLRSAFRRT
  SLAGAGRRTSDSHEDAGTLDFSSLLKKRDSFRRDSKLEAPAEEDVWEILRQAPPSEYERIAFQHGVTDLR
  GMLKRLKGMKQDEKKSTAFQKKLEPAYQVNKGHKIRLTVELADPDAEVKWLKNGQEIQMSGSKYIFESVG
  AKRTLTISQCSLADDAAYQCVVGGEKCSTELFVKEPPVLITRSLEDQLVMVGQRVEFECEVSEEGAQVKW
  LKDGVELTREETFKYRFKKDGRKHHLIINEATLEDAGHYAVRTSGGQSLAELIVQEKKLEVYQSIADLAV
  GAKDQAVFKCEVSDENVRGVWLKNGKELVPDNRIKVSHIGRVHKLTIDDVTPADEADYSFVPEGFACNLS
  AKLHFMEVKIDFVPRQEPPKIHLDCPGSTPDTIVVVAGNKLRLDVPISGDPAPTVVWQKTVTQGKKASTG
  PHPDAPEDAGADEEWVFDKKLLCETEGRVRVETTKDRSVFTVEGAEKEDEGVYTVTVKNPVGEDQVNLTV
  KVIDVPDAPAAPKISNVGEDSCTVQWEPPAYDGGQPVLGYILERKKKKSYRWMRLNFDLLRELSHEARRM
  IEGVAYEMRVYAVNAVGMSRPSPASQPFMPIGPPGEPTHLAVEDVSDTTVSLKWRPPERVGAGGLDGYSV
  EYCQEGCSEWTPALQGLTERTSMLVKDLPTGARLLFRVRAHNVAGPGGPIVTKEPVTVQEILQRPRLQLP
  RHLRQTIQKKVGEPVNLLIPFQGKPRPQVTWTKEGQPLAGEEVSIRNSPTDTILFIRAARRTHSGTYQVT
  VRIENMEDKATLILQIVDKPSPPQDIRIVETWGFNVALEWKPPQDDGNTEIWGYTVQKADKKTMEWFTVL
  EHYRRTHCVVSELIIGNGYYFRVFSHNMVGSSDKAAATKEPVFIPRPGITYEPPKYKALDFSEAPSFTQP
  LANRSIIAGYNAILCCAVRGSPKPKISWFKNGLDLGEDARFRMFCKQGVLTLEIRKPCPYDGGVYVCRAT
  NLQGEAQCECRLEVRVPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008653
• ORF Size: 3834 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008653.2, NP_032679.2
• RefSeq Size: 4163 bp
• RefSeq ORF: 3837 bp
• Locus ID: 17868
• Cytogenetics: 2 E1
• MW: 141.8 kDa