Smad6 (NM_008542) Mouse Tagged ORF Clone

CAT#: MR227576

Smad6 (Myc-DDK-tagged) - Mouse MAD homolog 6 (Drosophila) (Smad6)

Product Images

Circular map for MR227576

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smad6
• Synonyms: b2b390Clo; Madh6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227576 representing NM_008542
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCAGATCTCAAGCTT
  
  AAGCTTATGTTCAGGTCTAAACGTTCGGGGCTGGTGCGGCGACTTTGGCGAAGTCGTGTGGTCCCTGATC
  GGGAGGAAGGCAGCGGCGGCGGCGGTGGTGTCGACGAGGATGGGAGCCTGGGCAGCCGAGCTGAGCCTGC
  CCCGCGGGCACGAGAGGGCGGAGGCTGCAGCCGCTCCGAAGTCCGCTCGGTAGCCCCGCGGCGGCCCCGG
  GACGCGGTGGGACCGCGAGGCGCCGCGATCGCGGGCAGGCGCCGGCGCACAGGGGGCCTCCCGAGGCCCG
  TGTCGGAGTCGGGGGCCGGGGCTGGGGGCTCCCCGCTGGATGTGGCGGAGCCTGGAGGCCCAGGCTGGCT
  GCCTGAGAGTGACTGCGAGACGGTGACCTGCTGTCTCTTCTCCGAACGGGACGCAGCAGGCGCGCCCCGG
  GACTCTGGCGATCCCCAAGCCAGACAGTCCCCGGAGCCGGAGGAGGGCGGCGGGCCTCGGAGTCGCGAAG
  CCCGCTCGCGACTGCTGCTTCTGGAGCAGGAGCTCAAGACGGTCACGTACTCGCTGCTCAAGAGGCTCAA
  GGAGCGTTCGCTGGACACGCTGTTGGAGGCTGTGGAGTCCCGAGGCGGCGTACCGGGCGGCTGCGTGCTG
  GTGCCGCGCGCCGACCTCCGCTTGGGCGGCCAGCCCGCGCCACCGCAGCTGCTGCTCGGCCGCCTCTTCC
  GCTGGCCAGACCTGCAGCACGCAGTGGAGCTGAAACCCCTGTGCGGCTGCCACAGCTTTACCGCCGCCGC
  CGACGGGCCCACGGTGTGTTGCAACCCCTACCACTTCAGCCGGCTCTGCGGGCCAGAATCACCGCCGCCC
  CCCTATTCTCGGCTGTCTCCTCCTGACCAGTACAAGCCACTGGATCTGTCCGATTCTACATTGTCTTACA
  CTGAAACCGAGGCCACCAACTCCCTCATCACTGCTCCGGGTGAATTCTCAGATGCCAGCATGTCTCCGGA
  TGCCACCAAGCCGAGCCACTGGTGCAGCGTGGCGTACTGGGAGCACCGGACACGCGTGGGCCGCCTCTAT
  GCGGTGTACGACCAGGCTGTCAGCATTTTCTACGACCTACCTCAGGGCAGCGGCTTCTGCCTGGGCCAGC
  TCAACCTGGAGCAGCGCAGTGAGTCGGTGCGGCGCACGCGCAGCAAGATCGGTTTTGGCATACTGCTCAG
  CAAGGAGCCAGACGGCGTGTGGGCCTACAACCGGGGCGAGCACCCCATCTTCGTCAACTCCCCGACGCTG
  GATGCGCCCGGAGGCCGCGCCCTGGTCGTGCGCAAGGTGCCACCGGGTTACTCCATCAAGGTGTTCGACT
  TTGAGCGCTCAGGGCTGCTGCAGCACGCAGACGCCGCTCACGGCCCCTACGACCCGCACAGTGTGCGCAT
  CAGCTTCGCCAAGGGCTGGGGACCCTGCTACTCGCGACAGTTCATCACCTCCTGCCCCTGTTGGCTGGAG
  ATCCTACTCAACAACCACAGAAGCGGACCG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227576 representing NM_008542
  Red=Cloning site Green=Tags(s)
  
  KLMFRSKRSGLVRRLWRSRVVPDREEGSGGGGGVDEDGSLGSRAEPAPRAREGGGCSRSEVRSVAPRRPR
  DAVGPRGAAIAGRRRRTGGLPRPVSESGAGAGGSPLDVAEPGGPGWLPESDCETVTCCLFSERDAAGAPR
  DSGDPQARQSPEPEEGGGPRSREARSRLLLLEQELKTVTYSLLKRLKERSLDTLLEAVESRGGVPGGCVL
  VPRADLRLGGQPAPPQLLLGRLFRWPDLQHAVELKPLCGCHSFTAAADGPTVCCNPYHFSRLCGPESPPP
  PYSRLSPPDQYKPLDLSDSTLSYTETEATNSLITAPGEFSDASMSPDATKPSHWCSVAYWEHRTRVGRLY
  AVYDQAVSIFYDLPQGSGFCLGQLNLEQRSESVRRTRSKIGFGILLSKEPDGVWAYNRGEHPIFVNSPTL
  DAPGGRALVVRKVPPGYSIKVFDFERSGLLQHADAAHGPYDPHSVRISFAKGWGPCYSRQFITSCPCWLE
  ILLNNHRSGP
  
  
• Restriction Sites: HindIII-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008542
• ORF Size: 1500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2971 bp
• RefSeq ORF: 1488 bp
• Locus ID: 17130
• UniProt ID: O35182
• Cytogenetics: 9 C
• MW: 54.2 kDa
• Gene Summary: Binds to regulatory elements in target promoter regions (By similarity). May block the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding (By similarity). Acts as a mediator of TGF-beta and BMP antiflammatory activity. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes.[UniProtKB/Swiss-Prot Function]