Eln (NM_007925) Mouse Tagged ORF Clone

CAT#: MR227515

Eln (Myc-DDK-tagged) - Mouse elastin (Eln)

Product Images

Circular map for MR227515

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eln
• Synonyms: AI385707; AI480567; E030024M20Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227515 representing NM_007925
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGTCTGACAGCGGTAGTCCCGCAGCCTGGCGTCTTGCTGATCCTCTTGCTCAACCTCCTCCATC
  CCGCGCAGCCTGGAGGGGTTCCAGGAGCTGTGCCTGGCGGACTTCCTGGTGGAGTTCCCGGTGGAGTCTA
  TTATCCAGGGGCTGGTATTGGAGGCCTGGGAGGAGGAGGAGGAGCTCTGGGACCTGGAGGAAAACCACCT
  AAGCCAGGTGCCGGACTTCTGGGAACGTTTGGAGCAGGTCCTGGAGGACTTGGAGGTGCTGGCCCGGGTG
  CAGGTCTCGGGGCCTTTCCTGCAGGCACCTTCCCAGGGGCAGGAGCTCTGGTGCCCGGGGGAGCAGCAGG
  GGCTGCTGCGGCTTATAAAGCTGCCGCCAAAGCTGGGGCTGGGCTTGGTGGCGTTGGCGGAGTCCCAGGT
  GGTGTTGGCGTTGGTGGAGTTCCAGGTGGTGTTGGAGTTGGCGGAGTCCCAGGTGGTGTTGGAGTTGGTG
  GAGTCCCTGGCGGTGTTGGTGGTATTGGTGGCATCGGTGGCTTAGGAGTCTCGACAGGTGCTGTGGTGCC
  CCAAGTCGGAGCTGGCATCGGAGCTGGAGGAAAGCCTGGGAAAGTTCCTGGTGTTGGTCTTCCAGGTGTA
  TACCCAGGCGGAGTGCTCCCAGGAACAGGAGCTCGGTTCCCTGGTGTGGGGGTGCTCCCTGGAGTTCCCA
  CTGGCACAGGAGTCAAAGCCAAGGCTCCAGGTGGAGGTGGTGCTTTTGCTGGAATCCCAGGGGTCGGACC
  CTTTGGGGGTCAGCAGCCTGGTGTCCCACTGGGTTATCCCATCAAAGCACCAAAGCTGCCAGGTGGCTAC
  GGACTGCCCTATACCAATGGGAAATTGCCCTATGGAGTAGCTGGTGCAGGGGGCAAGGCTGGCTACCCAA
  CAGGGACAGGGGTCGGATCCCAGGCGGCGGCGGCAGCAGCTAAAGCAGCCAAGTATGGTGCTGGGGGAGC
  TGGAGTCCTCCCTGGTGTTGGAGGGGGTGGCATTCCTGGTGGTGCTGGCGCAATTCCTGGAATTGGAGGC
  ATTGCAGGCGCTGGAACTCCTGCAGCAGCAGCTGCTGCAAAGGCTGCTGCTAAGGCTGCTAAGTATGGAG
  CTGCTGGAGGTTTAGTGCCTGGTGGACCAGGAGTTAGGCTCCCAGGTGCTGGAATCCCAGGTGTTGGTGG
  CATTCCTGGTGTTGGTGGCATCCCAGGTGTTGGGGGCCCTGGTATTGGAGGTCCAGGCATTGTGGGTGGA
  CCAGGAGCTGTGTCACCAGCTGCAGCTGCTAAAGCTGCTGCCAAAGCTGCCAAATACGGAGCCAGAGGTG
  GAGTTGGCATCCCGACATATGGGGTTGGTGCTGGTGGCTTTCCTGGCTATGGTGTTGGAGCTGGAGCAGG
  ACTTGGAGGTGCAAGCCCAGCTGCTGCTGCTGCCGCCGCCAAAGCTGCTAAGTATGGTGCTGGAGGAGCT
  GGAGCCCTGGGAGGCCTGGTGCCAGGTGCAGTACCAGGTGCACTGCCAGGTGCAGTACCAGCTGTGCCGG
  GAGCTGGTGGAGTGCCAGGAGCAGGTACCCCTGCAGCTGCAGCTGCTGCCGCCGCCGCTAAAGCAGCCGC
  CAAAGCAGGTTTGGGTCCTGGTGTTGGTGGGGTTCCTGGTGGAGTTGGTGTTGGTGGGATTCCCGGTGGA
  GTTGGTGTTGGTGGGGTTCCTGGTGGAGTTGGCCCTGGTGGTGTTACTGGTATTGGAGCTGGTCCTGGCG
  GTCTTGGAGGAGCAGGGTCACCGGCTGCCGCTAAATCTGCTGCTAAGGCAGCTGCCAAAGCCCAGTACAG
  AGCTGCCGCTGGGCTTGGAGCTGGTGTCCCTGGATTTGGGGCTGGTGCTGGTGTCCCCGGATTTGGGGCT
  GGTGCTGGTGTCCCCGGATTTGGGGCTGGTGCTGGTGTCCCCGGATTTGGGGCTGGTGCTGGTGTCCCTG
  GATTTGGAGCTGGAGCAGTACCTGGATCGCTGGCTGCATCCAAAGCTGCTAAATATGGAGCAGCAGGTGG
  CCTTGGTGGCCCTGGAGGTCTCGGTGGCCCTGGAGGTCTCGGTGGACCTGGAGGACTTGGTGGGGCTGGT
  GTTCCCGGTAGAGTAGCAGGAGCTGCACCCCCTGCTGCTGCCGCTGCTGCTGCCAAAGCTGCTGCTAAGG
  CTGCCCAGTATGGCCTTGGTGGAGCCGGAGGATTGGGAGCCGGTGGACTGGGGGCCGGTGGACTGGGAGC
  CGGTGGACTGGGAGCTGGTGGACTGGGAGCCGGTGGACTGGGAGCTGGTGGACTGGGAGCCGGTGGACTG
  GGAGCTGGTGGAGGTGTGTCCCCTGCTGCAGCTGCTAAGGCAGCCAAATATGGTGCTGCTGGCCTTGGAG
  GTGTCCTAGGAGCCAGGCCATTCCCAGGTGGAGGAGTTGCAGCAAGACCTGGCTTTGGACTTTCTCCCAT
  TTATCCAGGTGGTGGTGCTGGGGGCCTGGGAGTTGGTGGAAAACCCCCGAAGCCCTATGGAGGAGCCCTT
  GGAGCCCTGGGATACCAAGGTGGGGGCTGCTTTGGGAAATCCTGTGGCCGGAAGAGAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227515 representing NM_007925
  Red=Cloning site Green=Tags(s)
  
  MAGLTAVVPQPGVLLILLLNLLHPAQPGGVPGAVPGGLPGGVPGGVYYPGAGIGGLGGGGGALGPGGKPP
  KPGAGLLGTFGAGPGGLGGAGPGAGLGAFPAGTFPGAGALVPGGAAGAAAAYKAAAKAGAGLGGVGGVPG
  GVGVGGVPGGVGVGGVPGGVGVGGVPGGVGGIGGIGGLGVSTGAVVPQVGAGIGAGGKPGKVPGVGLPGV
  YPGGVLPGTGARFPGVGVLPGVPTGTGVKAKAPGGGGAFAGIPGVGPFGGQQPGVPLGYPIKAPKLPGGY
  GLPYTNGKLPYGVAGAGGKAGYPTGTGVGSQAAAAAAKAAKYGAGGAGVLPGVGGGGIPGGAGAIPGIGG
  IAGAGTPAAAAAAKAAAKAAKYGAAGGLVPGGPGVRLPGAGIPGVGGIPGVGGIPGVGGPGIGGPGIVGG
  PGAVSPAAAAKAAAKAAKYGARGGVGIPTYGVGAGGFPGYGVGAGAGLGGASPAAAAAAAKAAKYGAGGA
  GALGGLVPGAVPGALPGAVPAVPGAGGVPGAGTPAAAAAAAAAKAAAKAGLGPGVGGVPGGVGVGGIPGG
  VGVGGVPGGVGPGGVTGIGAGPGGLGGAGSPAAAKSAAKAAAKAQYRAAAGLGAGVPGFGAGAGVPGFGA
  GAGVPGFGAGAGVPGFGAGAGVPGFGAGAVPGSLAASKAAKYGAAGGLGGPGGLGGPGGLGGPGGLGGAG
  VPGRVAGAAPPAAAAAAAKAAAKAAQYGLGGAGGLGAGGLGAGGLGAGGLGAGGLGAGGLGAGGLGAGGL
  GAGGGVSPAAAAKAAKYGAAGLGGVLGARPFPGGGVAARPGFGLSPIYPGGGAGGLGVGGKPPKPYGGAL
  GALGYQGGGCFGKSCGRKRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007925
• ORF Size: 2580 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007925.1, NM_007925.2, NM_007925.3, NM_007925.4, NP_031951.2
• RefSeq Size: 3781 bp
• RefSeq ORF: 2583 bp
• Locus ID: 13717
• UniProt ID: P54320
• Cytogenetics: 5 74.76 cM
• MW: 72.4 kDa
• Gene Summary: This gene encodes elastin, the extracellular matrix protein that forms a major structural component of several tissues including lungs and arterial walls. Cleavage of the signal peptide from the encoded precursor generates soluble tropoelastin which undergoes lysine-derived crosslinking to form elastin polymers. Mice lacking the encoded protein exhibit defective lung development, and die of an obstructive arterial disease resulting from subendothelial cell proliferation and reorganization of smooth muscle. [provided by RefSeq, Aug 2015]