Iqgap1 (NM_016721) Mouse Tagged ORF Clone

CAT#: MR227478

Iqgap1 (Myc-DDK-tagged) - Mouse IQ motif containing GTPase activating protein 1 (Iqgap1)

Product Images

Circular map for MR227478

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Iqgap1
• Synonyms: AA682088; D7Ertd237e; D7Ertd257e; mKIAA0051
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227478 representing NM_016721
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCCGCGGAGGAGGTTGATGGCCTGGGTGTGGTCCGGCCGCACTATGGCTCCGTCCTGGATAATG
  AGAGGCTCACTGCAGAGGAGATGGATGAGCGGAGACGGCAGAACGTGGCTTATGAATACCTTTGTCATCT
  GGAAGAAGCAAAGAGGTGGATGGAAGCATGCCTAGGTGAGGACCTGCCGCCCACCACAGAGCTAGAGGAG
  GGCCTTAGAAACGGAGTCTACCTTGCCAAGCTAGGGAACTTCTTCTCTCCCAAAGTGGTGTCCCTGAAGA
  AAATCTATGATCGAGAACAGACCAGATACAAGGCTACCGGCCTCCACTTCAGACACACGGATAATGTGAT
  TCAGTGGCTGAATGCCATGGATGAGATTGGGTTGCCTAAGATTTTTTACCCAGAAACCACAGATATCTAT
  GACCGGAAGAACATGCCAAGATGCATCTACTGTATCCACGCCCTCAGTTTGTACCTGTTCAAACTGGGCC
  TGGCTCCTCAGATTCAAGACCTGTATGGAAAGGTTGATTTCACAGAAGAAGAAATCAACAACATGAAGAT
  CGAGCTGGAGAAGTACGGGATCCAGATGCCTGCCTTCAGCAAGATCGGGGGCATCCTGGCTAATGAGCTC
  TCAGTGGATGAAGCTGCGCTACATGCTGCTGTTATTGCTATTAATGAAGCGATTGATCGCAGAGTTGCAG
  CTGACACTTTTACGGCTCTAAAAAACCCCAATGCCATGCTCGTCAATCTTGAAGAAGGCCTGGCTCCCAC
  GTACCAAGACGTGCTTTACCAGGCCAAGCAGGACAAGATGACAAACGCTAAAAACAGGACGGAAAACTCT
  GACAGAGAAAGGGACGTTTATGAGGAGCTGCTCACACAAGCTGAAATCCAAGGGAATGTAAACAAAGTCA
  ACACATCTTCTGCCCTGGCCAACATCAGCCTGGCTTTAGAGCAGGGCTGTGCAGTGACCCTGCTCAAGGC
  TCTGCAGTCACTGGCTCTGGGCCTCCGAGGGCTGCAGACCCAGAACAGCGACTGGTACATGAAGCAGCTA
  CAGAGTGATCTGCAGCAAAAGAGACAGAGTGGCCAGACTGACCCCCTGCAGAAGGAGGAGGTACAGGCCG
  GAGTGGATGCTGCCAACAGTGCTGCCCAGCAGTACCAACGACGGTTGGCAGCAGTGGCAGCAATCAACGC
  TGCCATCCAGAAGGGCATCGCTGAGAAGACCGTGTTGGAGCTAATGAATCCTGAAGCCCAGCTGCCCCAG
  GTGTATCCATTTGCAGCTGATCTCTATCAGAAGGAGTTGGCCACCCTGCAGCAGCAGAGCCCTGAGCATA
  GCCTCACCCATCCTGAGCTCACTGTTGCTGTGGAGATGCTGTCATCCGTGGCCCTCATCAACAGGGCGCT
  GGAGTCAGGAGACATGACCACTGTGTGGAAGCAGCTGAGCAGCTCAGTTACGGGCCTTACCAACATCGAG
  GAAGAAAACTGTCAAAGGTATCTCGATGAGCTGATGAAGCTGAAGGCTCAGGCACATGCCGAGAATAATG
  CATTTATTACATGGAATGACATCCAGGCGTGTGTGGACCATGTGAACCTGGTGGTCCATGAGGAGCATGA
  GCGGATTTTGGCCATCGGCTTGATTAATGAAGCCCTGGATGAAGGGGACGCTCAGAAGACTCTGCAGGCC
  CTGCAGATCCCTGCAGCCAAGCTCGAGGGCGTCCTTGCAGAAGTGGCACAGCACTATCAAGACACGCTGA
  TCAGAGCAAAGAGAGAAAAGGCCCAGGAAACACAGGATGAGTCAGCTGTGTTATGGTTGGATGAAATTCA
  AGGTGGAATCTGGCAGTCCAACAAAGACACCCAAGAGGCCCAGAGGTTTGCCTTAGGAATCTCTGCCATC
  AATGAAGCAGTAGACAGCGGTGATGTTGGCAGAACCCTGAGTGCCCTACGTTCTCCCGACGTTGGCTTAT
  ATGGAGTGATCCCCGAATGTGGGGAAACGTACCAGAGTGACCTTGCTGAAGCCAAGAAGAAGAGACTGGC
  AGCAGGAGATAATAACAGCAAGTGGGTGAAGCACTGGGTGAAAGGCGGGTACCATTACTACCACAACCTG
  GAGACGCAAGCAGGAGGATGGGCTGAGCCCCCAGACTTTGTGCAGAATTCTGTGCAGCTTTCTCGAGAGG
  AGATCCAGAGCTCCATCTCTGGAGTAACCGCTGCATATAACCGAGAGCAGCTTTGGCTGGCCAACGAAGG
  CTTGATCACCAAGCTGCAAGCCTGCTGCCGTGGGTACCTCGTTCGACAGGAATTCCGATCCCGGATGAAT
  TTTCTGAAGAAACAGATCCCTGCCATCACCTGCATTCAGTCACAGTGGAGAGGATACAAACAGAAGAAGG
  CATATCAAGATCGGCTGGCTTACCTGCACTCCCATAAAGACGAAGTTGTGAAGATTCAGTCCCTTGCCAG
  GATGCATCAAGCTCGAAAGCGCTATAGAGATCGCCTACAGTATTTCCGAGACCATATAAATGACATTATC
  AAAATCCAGGCTTTCATTCGGGCCAACAAAGCTCGTGATGACTACAAGACTCTCATCAATGCTGAGGACC
  CGCCTATGATTGTGGTCCGAAAGTTTGTCCACCTCCTGGACCAAAGTGATCAGGACTTCCAGGAGGAACT
  TGATCTCATGAAGATGCGCGAGGAGGTCATCACCCTCATCCGTTCCAACCAGCAGCTGGAGAACGACCTC
  AACCTCATGGATATCAAAATCGGACTGCTGGTGAAGAACAAGATCACGCTGCAGGATGTGGTTTCCCATA
  GTAAAAAACTTACCAAAAAAAATAAGGAACAGCTGTCCGACATGATGATGATAAACAAGCAGAAGGGCGG
  GCTCAAGGCTTTGAGCAAAGAGAAGAGGGAGAAGCTGGAGGCCTATCAGCATCTCTTTTATCTCCTGCAG
  ACCAACCCTACCTATCTGGCCAAGCTGATCTTTCAGATGCCACAAAACAAGTCCACCAAATTCATGGACT
  CTGTGATCTTCACGCTGTACAACTATGCATCTAACCAGCGGGAGGAGTACCTGCTGCTGCGGCTCTTCCA
  GACAGCTCTGCAGGAGGAGATCAAGTCAAAGGTGGATCAGATTCAAGAAATCGTGACAGGAAACCCTACG
  GTTATTAAGATGGTTGTAAGTTTCAACCGTGGTGCCCGGGGCCAGAATGCCCTCCGGCAGATCTTGGCCC
  CTGTCGTGAAGGAAATTATGGATGACAAGTCTCTCAACATCAAAACCGACCCTGTGGATATTTACAAGTC
  TTGGGTTAATCAGATGGAGTCGCAGACAGGAGAGGCGAGCAAACTGCCCTATGATGTGACTCCTGAACAA
  GCCTTGTCTCATGAAGAAGTGAAGACGAGGTTAGACAACTCCATCAGGAACATGAGGGCCGTGACAGACA
  AGTTCCTCTCAGCCATCGTCAGCTCTGTGGACAAAATCCCTTATGGGATGCGCTTCATTGCCAAAGTCCT
  GAAGGATTCCCTGCACGAGAAGTTCCCTGACGCTGGTGAGGACGAGCTGCTGAAGATTATCGGTAACCTG
  CTTTACTACCGATACATGAACCCAGCCATCGTCGCTCCCGATGCCTTCGACATCATTGACCTGTCAGCAG
  GGGGCCAGCTCACCACAGACCAGCGCAGAAACCTGGGCTCCATTGCCAAGATGCTCCAGCACGCGGCGTC
  CAACAAGATGTTTCTGGGCGATAATGCCCACTTAAGCATCATTAATGAGTATCTCTCGCAGTCCTACCAG
  AAATTCAGACGGTTTTTCCAAGTGGCTTGTGACGTCCCAGAGCTGCAGGATAAATTTAACGTGGATGAGT
  ACTCTGACCTAGTCACCCTCACTAAGCCAGTTATCTACATCTCCATTGGCGAAATCATCAACACCCACAC
  TCTCCTGTTGGACCATCAGGATGCCATTGCTCCAGAGCATAACGACCCCATCCACGAACTTCTGGACGAC
  CTTGGGGAGGTGCCCACCATTGAGTCCCTTATAGGAGAAAGCTGTGGCAATTCAAACGACCCCAACAAGG
  AGGCTCTGGCTAAGACGGAAGTGTCTCTCACGTTGACCAACAAGTTTGACGTGCCTGGTGACGAGAACGC
  AGAGATGGACGCTCGGACCATCTTACTGAATACAAAACGTTTAATTGTGGATGTCATCCGGTTCCAGCCA
  GGAGAGACCTTGACTGAAATTCTAGAAACCCCAGCCACCAATGAACAGGAAGCTGAACATCAGAGGGCCA
  TGCAGAGACGGGCTATCCGCGATGCCAAAACCCCTGACAAGATGAAAAAATCAAAGCCCATGAAGGAGGA
  TAACAACCTCAGCCTCCAGGAGAAGAAAGAGAAGATCCAGACTGGCCTAAAGAAGCTAACGGAGCTTGGG
  ACGGTGGACCCAAAGAACAGATACCAGGAACTCATCAACGACATTGCCAAGGATATCCGGAATCAGCGGA
  GATACAGGCAGAGGAGGAAAGCTGAATTGGTAAAACTGCAGCAGACGTACTCGGCGCTGAACTCTAAGGC
  CACCTTTTACGGCGAGCAGGTGGACTACTACAAGAGCTACATCAAAACCTGCTTGGATAACTTGGCCAGC
  AAGGGCAAGGTCTCCAAAAAGCCTAGGGAAATGAAAGGCAAGAAAAGCAAAAAGATTTCTCTGAAGTACA
  CAGCAGCGAGGCTGCATGAGAAGGGCGTCCTTCTGGAGATTGAAGACCTTCAGGCAAACCAATTTAAAAA
  TGTTATCTTCGAAATTGGTCCAACAGAAGAAGTTGGAGACTTTGAAGTAAAAGCCAAGTTCATGGGAGTT
  CAGATGGAGACTTTCATGTTGCATTATCAGGACTTGCTGCAGCTACAGTATGAAGGAGTTGCAGTTATGA
  AATTATTTGATAGAGCTAAAGTGAATGTCAACCTCCTGATCTTCCTTCTCAACAAAAAGTTCTATGGGAA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227478 representing NM_016721
  Red=Cloning site Green=Tags(s)
  
  MSAAEEVDGLGVVRPHYGSVLDNERLTAEEMDERRRQNVAYEYLCHLEEAKRWMEACLGEDLPPTTELEE
  GLRNGVYLAKLGNFFSPKVVSLKKIYDREQTRYKATGLHFRHTDNVIQWLNAMDEIGLPKIFYPETTDIY
  DRKNMPRCIYCIHALSLYLFKLGLAPQIQDLYGKVDFTEEEINNMKIELEKYGIQMPAFSKIGGILANEL
  SVDEAALHAAVIAINEAIDRRVAADTFTALKNPNAMLVNLEEGLAPTYQDVLYQAKQDKMTNAKNRTENS
  DRERDVYEELLTQAEIQGNVNKVNTSSALANISLALEQGCAVTLLKALQSLALGLRGLQTQNSDWYMKQL
  QSDLQQKRQSGQTDPLQKEEVQAGVDAANSAAQQYQRRLAAVAAINAAIQKGIAEKTVLELMNPEAQLPQ
  VYPFAADLYQKELATLQQQSPEHSLTHPELTVAVEMLSSVALINRALESGDMTTVWKQLSSSVTGLTNIE
  EENCQRYLDELMKLKAQAHAENNAFITWNDIQACVDHVNLVVHEEHERILAIGLINEALDEGDAQKTLQA
  LQIPAAKLEGVLAEVAQHYQDTLIRAKREKAQETQDESAVLWLDEIQGGIWQSNKDTQEAQRFALGISAI
  NEAVDSGDVGRTLSALRSPDVGLYGVIPECGETYQSDLAEAKKKRLAAGDNNSKWVKHWVKGGYHYYHNL
  ETQAGGWAEPPDFVQNSVQLSREEIQSSISGVTAAYNREQLWLANEGLITKLQACCRGYLVRQEFRSRMN
  FLKKQIPAITCIQSQWRGYKQKKAYQDRLAYLHSHKDEVVKIQSLARMHQARKRYRDRLQYFRDHINDII
  KIQAFIRANKARDDYKTLINAEDPPMIVVRKFVHLLDQSDQDFQEELDLMKMREEVITLIRSNQQLENDL
  NLMDIKIGLLVKNKITLQDVVSHSKKLTKKNKEQLSDMMMINKQKGGLKALSKEKREKLEAYQHLFYLLQ
  TNPTYLAKLIFQMPQNKSTKFMDSVIFTLYNYASNQREEYLLLRLFQTALQEEIKSKVDQIQEIVTGNPT
  VIKMVVSFNRGARGQNALRQILAPVVKEIMDDKSLNIKTDPVDIYKSWVNQMESQTGEASKLPYDVTPEQ
  ALSHEEVKTRLDNSIRNMRAVTDKFLSAIVSSVDKIPYGMRFIAKVLKDSLHEKFPDAGEDELLKIIGNL
  LYYRYMNPAIVAPDAFDIIDLSAGGQLTTDQRRNLGSIAKMLQHAASNKMFLGDNAHLSIINEYLSQSYQ
  KFRRFFQVACDVPELQDKFNVDEYSDLVTLTKPVIYISIGEIINTHTLLLDHQDAIAPEHNDPIHELLDD
  LGEVPTIESLIGESCGNSNDPNKEALAKTEVSLTLTNKFDVPGDENAEMDARTILLNTKRLIVDVIRFQP
  GETLTEILETPATNEQEAEHQRAMQRRAIRDAKTPDKMKKSKPMKEDNNLSLQEKKEKIQTGLKKLTELG
  TVDPKNRYQELINDIAKDIRNQRRYRQRRKAELVKLQQTYSALNSKATFYGEQVDYYKSYIKTCLDNLAS
  KGKVSKKPREMKGKKSKKISLKYTAARLHEKGVLLEIEDLQANQFKNVIFEIGPTEEVGDFEVKAKFMGV
  QMETFMLHYQDLLQLQYEGVAVMKLFDRAKVNVNLLIFLLNKKFYGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016721
• ORF Size: 4971 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016721.2, NP_057930.2
• RefSeq Size: 7377 bp
• RefSeq ORF: 4974 bp
• Locus ID: 29875
• UniProt ID: Q9JKF1
• Cytogenetics: 7 45.68 cM
• MW: 189.2 kDa
• Gene Summary: Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Binds to activated CDC42 but does not stimulate its GTPase activity (PubMed:16968698). It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. May play a possible role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest.[UniProtKB/Swiss-Prot Function]