Tshr (NM_001113404) Mouse Tagged ORF Clone

CAT#: MR227475

Tshr (Myc-DDK-tagged) - Mouse thyroid stimulating hormone receptor (Tshr), transcript variant 2

Product Images

Circular map for MR227475

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tshr
• Synonyms: AI481368; hypothroid; hyt; pet
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227475 representing NM_001113404
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCCAGGGTCCCTGCTGCTGCTTGTTCTGCTGCTCGCCCTGTCCAGGAGCCTGCGGGGCAAAGAGT
  GTGCGTCTCCACCCTGTGAGTGTCACCAGGAGGACGACTTCAGAGTCACCTGCAAGGAGCTCCACCGAAT
  CCCCAGCCTGCCGCCCAGCACCCAGACTCTGAAGCTCATCGAGACTCATCTGAAGACCATACCCAGTCTT
  GCATTTTCGAGTCTGCCCAATATTTCCAGGATCTATTTATCTATAGATGCAACTCTGCAGCGGCTGGAAC
  CACATTCTTTCTACAATTTGAGTAAAATGACTCACATAGAAATCCGGAACACCAGAAGCTTAACCTATAT
  AGACCCTGATGCCTTGACAGAGCTCCCCTTGCTCAAGTTTCTTGGCATTTTCAATACTGGACTTAGAATA
  TTCCCTGACTTGACCAAAATTTATTCCACGGACATATTCTTTATACTTGAAATCACAGACAACCCTTACA
  TGACTTCGGTCCCTGAAAACGCATTCCAGGGCCTATGCAATGAAACCTTGACCCTGAAACTGTACAACAA
  TGGATTTACTTCAGTCCAAGGACATGCTTTCAATGGAACAAAGCTGGATGCTGTTTACCTAAACAAGAAT
  AAATACCTGACAGCTATAGACAACGATGCCTTTGGAGGAGTATACAGTGGACCAACTTTGCTGTCATTTC
  TCAAAGAAGAATTTGATTTCCACCAGGCACAAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227475 representing NM_001113404
  Red=Cloning site Green=Tags(s)
  
  MRPGSLLLLVLLLALSRSLRGKECASPPCECHQEDDFRVTCKELHRIPSLPPSTQTLKLIETHLKTIPSL
  AFSSLPNISRIYLSIDATLQRLEPHSFYNLSKMTHIEIRNTRSLTYIDPDALTELPLLKFLGIFNTGLRI
  FPDLTKIYSTDIFFILEITDNPYMTSVPENAFQGLCNETLTLKLYNNGFTSVQGHAFNGTKLDAVYLNKN
  KYLTAIDNDAFGGVYSGPTLLSFLKEEFDFHQAQI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113404
• ORF Size: 735 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113404.1, NP_001106875.1
• RefSeq Size: 1346 bp
• RefSeq ORF: 738 bp
• Locus ID: 22095
• Cytogenetics: 12 44.51 cM
• MW: 28.1 kDa
• Gene Summary: Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Plays a central role in controlling thyroid cell metabolism.[UniProtKB/Swiss-Prot Function]