Sept4 (NM_011129) Mouse Tagged ORF Clone

CAT#: MR227466

41521 (Myc-DDK-tagged) - Mouse septin 4 (Sept4), nuclear gene encoding mitochondrial protein

Product Images

Circular map for MR227466

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sept4
• Synonyms: ARTS; Bh5; OTTMUSG00000001265; Pnutl2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227466 representing NM_011129
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCATTCACTGGGATGGCAAGGGAACTCTGTCCCCGAGGACGGGACTGAAGCTGGGATCAAGCACT
  TCCTGGAGGACAGCAGTGATGATGCTGAGCTGAGCAAGTTCGTGAAGGATTTCCCAGGAAGCGAACCCTA
  CCACTCAGCGGAGTCCAAGACAAGGGTGGCCAGGCCCCAGATCTTGGAGCCAAGGCCCCAGAGCCCAGAC
  CTCTGTGATGATGACGTGGAGTTTAGAGGCTCCTTGTGGCCCCAGCCCTCTGACAGTCAGCAGTACTTCA
  GTGCCCCAGCCCCTCTCAGCCCTTCCTCCAGGCCCCGCAGTCCATGGGGCAAGCTTGATCCTTATGATTC
  CTCTGAGGATGACAAGGAGTATGTGGGCTTTGCAACCCTCCCCAATCAAGTCCACAGGAAGTCTGTGAAG
  AAAGGCTTTGACTTTACACTCATGGTGGCAGGAGAATCTGGTCTGGGTAAATCCACTCTTGTCAACAGCC
  TCTTCCTCACTGACTTGTACCGGGATCGGAAACTGCTGGGCGCCGAAGAGCGGATCATGCAAACCGTGGA
  GATTACTAAGCACGCAGTGGATATAGAAGAGAAGGGAGTGAGGCTGCGGCTCACCATTGTGGACACTCCA
  GGATTTGGGGATGCAGTCAACAACACAGAGTGCTGGAAGCCTGTGGCCGAATACATCGACCAGCAGTTTG
  AGCAGTACTTCCGAGACGAGAGTGGCCTGAACCGCAAGAACATCCAGGACAACCGGGTGCACTGCTGCCT
  GTACTTCATCTCCCCGTTTGGCCACGGGCTCCGGCCATTGGATGTTGAATTCATGAAGGCCCTGCATCAG
  CGGGTCAACATTGTGCCTATCTTGGCTAAGGCGGACACACTGACGCCTCCTGAAGTGGACCGAAAGAAAT
  GCAAAATCCGGGAGGAGATCGAGCACTTTGGAATCAAGATCTATCAGTTCCCAGACTGTGATTCCGATGA
  GGACGAGGACTTCAAATTACAGGACCAAGCCCTAAAGGAAAGCATCCCATTTGCGGTGATTGGCAGCAAC
  ACTGTGGTAGAAGCCAGGGGGCGGAGAGTTCGAGGCCGCCTCTACCCTTGGGGCATCGTGGAAGTGGAAA
  ACCCAGGTCACTGCGACTTTGTCAAGTTGAGGACGATGCTGGTGCGTACCCACATGCAGGACCTAAAGGA
  TGTGACCCGAGAGACACACTACGAGAACTACAGGGCACAGTGTATCCAGAGCATGACCCGGCTAGTAGTG
  AAGGAACGGAATCGCAACAAACTGACAAGAGAGAGTGGTACTGACTTCCCTATCCCTGCTGTCCCACCAG
  GGACAGATCCAGAAACTGAGAAGCTAATCCGGGAGAAAGACGAAGAGCTGCGGCGGATGCAGGAGATGTT
  ACACAAAATCCAAAGACAGATGAAGGAGACTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227466 representing NM_011129
  Red=Cloning site Green=Tags(s)
  
  MDHSLGWQGNSVPEDGTEAGIKHFLEDSSDDAELSKFVKDFPGSEPYHSAESKTRVARPQILEPRPQSPD
  LCDDDVEFRGSLWPQPSDSQQYFSAPAPLSPSSRPRSPWGKLDPYDSSEDDKEYVGFATLPNQVHRKSVK
  KGFDFTLMVAGESGLGKSTLVNSLFLTDLYRDRKLLGAEERIMQTVEITKHAVDIEEKGVRLRLTIVDTP
  GFGDAVNNTECWKPVAEYIDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPLDVEFMKALHQ
  RVNIVPILAKADTLTPPEVDRKKCKIREEIEHFGIKIYQFPDCDSDEDEDFKLQDQALKESIPFAVIGSN
  TVVEARGRRVRGRLYPWGIVEVENPGHCDFVKLRTMLVRTHMQDLKDVTRETHYENYRAQCIQSMTRLVV
  KERNRNKLTRESGTDFPIPAVPPGTDPETEKLIREKDEELRRMQEMLHKIQRQMKETH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011129
• ORF Size: 1434 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011129.2, NP_035259.1
• RefSeq Size: 1649 bp
• RefSeq ORF: 1437 bp
• Locus ID: 18952
• UniProt ID: P28661
• Cytogenetics: 11 C
• MW: 55.4 kDa
• Gene Summary: Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion (By similarity).[UniProtKB/Swiss-Prot Function]