Mark3 (NM_021516) Mouse Tagged ORF Clone

CAT#: MR227363

Mark3 (Myc-DDK-tagged) - Mouse MAP/microtubule affinity-regulating kinase 3 (Mark3), transcript variant 1

Product Images

Circular map for MR227363

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mark3
• Synonyms: 1600015G02Rik; A430080F22Rik; C-TAK1; Emk2; ETK-1; ETK1; MPK10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227363 representing NM_021516
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACTAGGACCCCTTTGCCAACGGTGAATGAACGAGACACTGAAAACCACATTTCACATGGAGACG
  GGAGGCAAGAAGTCACCTCCCGCACCGGGCGCTCTGGAGCTCGGTGTAGAAACTCAATAGCTTCCTGTGC
  AGATGAACAGCCTCACATCGGAAACTACAGACTGTTGAAAACAATCGGCAAGGGGAACTTTGCAAAAGTG
  AAATTGGCGAGACACATCCTCACAGGCCGAGAGGTTGCAATAAAAATAATTGACAAAACTCAGTTGAATC
  CAACAAGTCTACAAAAGCTCTTCAGAGAAGTAAGAATAATGAAGATTTTAAATCACCCAAACATAGTGAA
  GTTGTTCGAAGTCATTGAAACGGAAAAAACTCTCTACTTAATCATGGAATATGCAAGTGGAGGTGAAGTA
  TTTGACTATTTGGTTGCACATGGAAGAATGAAGGAAAAAGAAGCAAGAGCTAAATTTAGACAGATTGTTT
  CCGCAGTACAGTATTGCCACCAAAAACGGATTGTTCACAGAGACCTCAAGGCTGAAAATCTGTTATTAGA
  TGCGGATATGAACATTAAAATAGCAGATTTTGGTTTTAGCAATGAATTTACTGTTGGCAGTAAACTAGAC
  ACGTTTTGTGGTAGTCCTCCATATGCAGCCCCTGAGCTCTTCCAGGGCAAGAAGTATGACGGGCCAGAAG
  TCGACGTGTGGAGCCTCGGTGTCATTCTCTACACCCTAGTCAGTGGGTCGCTTCCCTTTGATGGTCAAAA
  CCTGAAGGAACTGAGAGAGAGAGTACTCCGCGGGAAGTACAGAATCCCTTTCTACATGTCCACAGACTGC
  GAGAACCTTCTCAAGCGCTTCCTGGTGCTGAACCCAGTTAAACGGGGCACTCTCGAGCAAATCATGAAGG
  ACAGGTGGATCAATGCAGGCCATGAGGAGGATGAGCTTAAGCCATTTGTTGAACCAGAACTAGACATCTC
  AGACCAGAAGAGAATAGATATTATGGTGGGAATGGGATATTCCCAAGAAGAAATTCAAGAATCTCTTAGC
  AAAATGAAATACGATGAAATCACAGCTACTTACTTGCTACTGGGGAGGAAATCTGCAGAGCTGGACGCTA
  GTGACTCCAGTTCTAGCAGCAACCTTTCACTTGCTAAGGTGCGGCCAAACAGTGACCTCAGCAATAGCAC
  TGGCCAGTCTCCTCATCACAAAGGGCAGAGAAGTGTTTCCTCAAGTCAGAAGCAGAGACGGTACAGTGAC
  CACGCTGGACCAGCTATTCCTTCAGTTGTGGCATATCCAAAGAGGAGTCAGACCAGCACTGCAGATAGTG
  ACCTCAAAGAAGATGGAATTCCCTCCCGAAAATCAAGCAGCAGTGCTGTAGGAGGGAAGGGGATTGCTCC
  TGCCAGTCCCATGCTTGGGAATGCAGGCAATCCCAATAAAGCCGATATCCCTGAGCGCAAGAAAAGCCCT
  GCTGTGCCCAGTAGTAACACAGCATCTGGTGGGATGACACGGCGGAACACTTACGTTTGCAGCGAGAGAT
  GTGCAGCAGACAGACATTCTGTGATTCAGAACGGCAAAGAAAACAGCGCTATTCCAGATGAGAGAACTCC
  AGTTGCTTCAACCCACAGTATTAGCAGTGCGACCACCCCAGATCGCATCCGCTTTCCAAGGGGCACAGCC
  AGCCGTAGCACTTTCCATGGCCAACCTCGGGAGCGGCGAACCGCAACATATAATGGCCCACCTGCCTCAC
  CCAGCTTGTCCCATGAAGCCACACCGCTGTCTCAGACTCGAAGCCGAGGTTCCACTAATCTTTTTAGTAA
  ATTAACTTCCAAACTCACAAGAAGACTTCCAACTGAATATGAGAGGAACGGGAGATATGAGGGCTCAAGT
  CGCAATGTGTCTTCAGAGCAGAAGGATGAGAACAGAGAGGCCAAGCCCCGCTCCCTGCGCTTCACCTGGA
  GCATGAAGACCACGAGCTCCATGGACCCCAGTGACATGATGCGGGAGATCCGCAAGGTGCTGGACGCCAA
  TAACTGCGACTACGAGCAGAGGGAGCGCTTCCTGCTCTTCTGTGTCCATGGCGACGGGCACGCAGAGAAC
  CTGGTACAGTGGGAAATGGAAGTCTGTAAGCTGCCAAGACTGTCTCTGAATGGAGTCCGGTTTAAGCGGA
  TATCAGGGACATCCATAGCTTTCAAAAATATTGCTTCCAAAATTGCCAATGAGCTAAAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227363 representing NM_021516
  Red=Cloning site Green=Tags(s)
  
  MSTRTPLPTVNERDTENHISHGDGRQEVTSRTGRSGARCRNSIASCADEQPHIGNYRLLKTIGKGNFAKV
  KLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLIMEYASGGEV
  FDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKRIVHRDLKAENLLLDADMNIKIADFGFSNEFTVGSKLD
  TFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDC
  ENLLKRFLVLNPVKRGTLEQIMKDRWINAGHEEDELKPFVEPELDISDQKRIDIMVGMGYSQEEIQESLS
  KMKYDEITATYLLLGRKSAELDASDSSSSSNLSLAKVRPNSDLSNSTGQSPHHKGQRSVSSSQKQRRYSD
  HAGPAIPSVVAYPKRSQTSTADSDLKEDGIPSRKSSSSAVGGKGIAPASPMLGNAGNPNKADIPERKKSP
  AVPSSNTASGGMTRRNTYVCSERCAADRHSVIQNGKENSAIPDERTPVASTHSISSATTPDRIRFPRGTA
  SRSTFHGQPRERRTATYNGPPASPSLSHEATPLSQTRSRGSTNLFSKLTSKLTRRLPTEYERNGRYEGSS
  RNVSSEQKDENREAKPRSLRFTWSMKTTSSMDPSDMMREIRKVLDANNCDYEQRERFLLFCVHGDGHAEN
  LVQWEMEVCKLPRLSLNGVRFKRISGTSIAFKNIASKIANELKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021516
• ORF Size: 2232 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021516.4, NP_067491.2
• RefSeq Size: 3388 bp
• RefSeq ORF: 2235 bp
• Locus ID: 17169
• UniProt ID: Q03141
• Cytogenetics: 12 F1
• MW: 83.7 kDa
• Gene Summary: Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAPT/TAU, MAP2 and MAP4. Phosphorylates CDC25C. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1.[UniProtKB/Swiss-Prot Function]