Mark3 (NM_021516) Mouse Untagged Clone

CAT#: MC221224

Mark3 (untagged) - Mouse MAP/microtubule affinity-regulating kinase 3 (Mark3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mark3
• Synonyms: 1600015G02Rik; A430080F22Rik; C-TAK1; Emk2; ETK-1; ETK1; MPK10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221224 representing NM_021516
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACTAGGACCCCTTTGCCAACGGTGAATGAACGAGACACTGAAAACCACATTTCACATGGAGACG
  GGAGGCAAGAAGTCACCTCCCGCACCGGGCGCTCTGGAGCTCGGTGTAGAAACTCAATAGCTTCCTGTGC
  AGATGAACAGCCTCACATCGGAAACTACAGACTGTTGAAAACAATCGGCAAGGGGAACTTTGCAAAAGTG
  AAATTGGCGAGACACATCCTCACAGGCCGAGAGGTTGCAATAAAAATAATTGACAAAACTCAGTTGAATC
  CAACAAGTCTACAAAAGCTCTTCAGAGAAGTAAGAATAATGAAGATTTTAAATCACCCAAACATAGTGAA
  GTTGTTCGAAGTCATTGAAACGGAAAAAACTCTCTACTTAATCATGGAATATGCAAGTGGAGGTGAAGTA
  TTTGACTATTTGGTTGCACATGGAAGAATGAAGGAAAAAGAAGCAAGAGCTAAATTTAGACAGATTGTTT
  CCGCAGTACAGTATTGCCACCAAAAACGGATTGTTCACAGAGACCTCAAGGCTGAAAATCTGTTATTAGA
  TGCGGATATGAACATTAAAATAGCAGATTTTGGTTTTAGCAATGAATTTACTGTTGGCAGTAAACTAGAC
  ACGTTTTGTGGTAGTCCTCCATATGCAGCCCCTGAGCTCTTCCAGGGCAAGAAGTATGACGGGCCAGAAG
  TCGACGTGTGGAGCCTCGGTGTCATTCTCTACACCCTAGTCAGTGGGTCGCTTCCCTTTGATGGTCAAAA
  CCTGAAGGAACTGAGAGAGAGAGTACTCCGCGGGAAGTACAGAATCCCTTTCTACATGTCCACAGACTGC
  GAGAACCTTCTCAAGCGCTTCCTGGTGCTGAACCCAGTTAAACGGGGCACTCTCGAGCAAATCATGAAGG
  ACAGGTGGATCAATGCAGGCCATGAGGAGGATGAGCTTAAGCCATTTGTTGAACCAGAACTAGACATCTC
  AGACCAGAAGAGAATAGATATTATGGTGGGAATGGGATATTCCCAAGAAGAAATTCAAGAATCTCTTAGC
  AAAATGAAATACGATGAAATCACAGCTACTTACTTGCTACTGGGGAGGAAATCTGCAGAGCTGGACGCTA
  GTGACTCCAGTTCTAGCAGCAACCTTTCACTTGCTAAGGTGCGGCCAAACAGTGACCTCAGCAATAGCAC
  TGGCCAGTCTCCTCATCACAAAGGGCAGAGAAGTGTTTCCTCAAGTCAGAAGCAGAGACGGTACAGTGAC
  CACGCTGGACCAGCTATTCCTTCAGTTGTGGCATATCCAAAGAGGAGTCAGACCAGCACTGCAGATAGTG
  ACCTCAAAGAAGATGGAATTCCCTCCCGAAAATCAAGCAGCAGTGCTGTAGGAGGGAAGGGGATTGCTCC
  TGCCAGTCCCATGCTTGGGAATGCAGGCAATCCCAATAAAGCCGATATCCCTGAGCGCAAGAAAAGCCCT
  GCTGTGCCCAGTAGTAACACAGCATCTGGTGGGATGACACGGCGGAACACTTACGTTTGCAGCGAGAGAT
  GTGCAGCAGACAGACATTCTGTGATTCAGAACGGCAAAGAAAACAGCGCTATTCCAGATGAGAGAACTCC
  AGTTGCTTCAACCCACAGTATTAGCAGTGCGACCACCCCAGATCGCATCCGCTTTCCAAGGGGCACAGCC
  AGCCGTAGCACTTTCCATGGCCAACCTCGGGAGCGGCGAACCGCAACATATAATGGCCCACCTGCCTCAC
  CCAGCTTGTCCCATGAAGCCACACCGCTGTCTCAGACTCGAAGCCGAGGTTCCACTAATCTTTTTAGTAA
  ATTAACTTCCAAACTCACAAGAAGACTTCCAACTGAATATGAGAGGAACGGGAGATATGAGGGCTCAAGT
  CGCAATGTGTCTTCAGAGCAGAAGGATGAGAACAGAGAGGCCAAGCCCCGCTCCCTGCGCTTCACCTGGA
  GCATGAAGACCACGAGCTCCATGGACCCCAGTGACATGATGCGGGAGATCCGCAAGGTGCTGGACGCCAA
  TAACTGCGACTACGAGCAGAGGGAGCGCTTCCTGCTCTTCTGTGTCCATGGCGACGGGCACGCAGAGAAC
  CTGGTACAGTGGGAAATGGAAGTCTGTAAGCTGCCAAGACTGTCTCTGAATGGAGTCCGGTTTAAGCGGA
  TATCAGGGACATCCATAGCTTTCAAAAATATTGCTTCCAAAATTGCCAATGAGCTAAAGCTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021516
• Insert Size: 2235 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021516.4, NP_067491.2
• RefSeq Size: 3388 bp
• RefSeq ORF: 2235 bp
• Locus ID: 17169
• UniProt ID: Q03141
• Cytogenetics: 12 F1
• Gene Summary: Serine/threonine-protein kinase. Involved in the specific phosphorylation of microtubule-associated proteins for MAPT/TAU, MAP2 and MAP4. Phosphorylates CDC25C. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).