Alox12 (NM_007440) Mouse Tagged ORF Clone

CAT#: MR227302

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  • TrueORF®

Alox12 (Myc-DDK-tagged) - Mouse arachidonate 12-lipoxygenase (Alox12)



  "NM_007440" in other vectors (4)

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USD 610.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Alox12 (NM_007440) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Alox12
Synonyms 9930022G08Rik; Alox12p; P-12LO
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR227302 representing NM_007440
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCGCTACCGCGTCCGTGTGGTCACTGGGGCCTGGCTCTTCTCCGGATCCCTCAACCTAGTGCGTT
TGTGGCTGGTTGGGGAGCACCGGGAGGCGAAACTGGAGCTGCAACTGCGGCCAGCGCGGGGCAAGGAGGA
GGAGTTTGACTTCGACGTTCCCGAGGACTTGGGGCCACTGCAGTTTGTGAAACTGCACAAACAGCACACA
GTTGTGGATGACGCCTGGTTCTGCAACCTCATCACAGTTCAGGGGCCGGGGACAAGTGCAGAGGCCGTGT
TTCCCTGCTACCGCTGGGTGCAGGGAGAGGGAATCCTGAGCCTCCCGGAGGGGACAGCCCGCCTGGCAGG
AGACAATGCCTTAGATGTCTTCCAGAAGTATCGAGAAAAGGAACTGAAGGAAAGACAACAGACCTACTGC
TGGGCCACCTGGAAAGAAGGCTTACCTCAGACAATAGCAGCGGACTGTAAGGATGACCTTCCTCCAAATA
TGAGATTCCATGAGGAGAAGAGACTGGACTTTGAATGGACGTTGAAGGCGGGGGTTCTGGAAATGGGCCT
CAAACGTGTTTATACCCTCCTGAGAAGCTGGAACCATCTGGAAGACTTTGATCAGATCTTCTGGGGCCAG
AAGAGTGCCTTGGCCGAGAAGGTTCACCAGTGTTGGCAGGAAGATGAACTCTTTGGCTACCAGTTCCTCA
ATGGCGCCAACCCCATGCTTTTGAGACGCTCCACCTCTCTGCCCTCCAGACTGGTACTGCCCTCTGGGAT
GGAGGAGCTTCAAGCTCAGTTGGAGAAAGAACTCAAGAATGGATCCCTGTTTGAAGCTGACTTTATCCTG
CTGGATGGAATTCCAGCTAATGTGATCCGAGGAGAACCACAGTACCTGGCTGCCCCTCTTGTCATGCTGA
GGATGGACCCCGGTGGGAAGCTGCTACCCATGGCTATCCAGATTCAGCCCCCTAACCCCAGCTCCCCAGC
TCCAACACTGTTCCTGCCCTCGGATCCTCCACTTGCCTGGCTCTTGGCTAAGATCTGGGTCCGAAATTCA
GATTTCCAACTGCAGGAGCTCCAATTCCATTTGCTGAACACCCATCTGGTGGCTGAGGTCATCGCTGTTG
CCACCATGAGATGCCTCCCAGGACTGCACCCTATCTTCAAGCTCCTCGTTCCACACATCCGTTACACTAT
GGAAATTAACACGCGAAGCCGGACCCAGCTCATCTCAGATGGAGGAATATTTGATCAGGTAGTGAGCACA
GGTGGAGGCGGCCATGTTCAGTTGCTTACTCGGGCAGTGGCTCAGCTGACTTACCACTCCCTCTGTCCTC
CTGATGATCTGGCTAACCGAGGCCTGCTGAGAATCCCAAGTGCTCTCTATGCTCGGGATGCTTTACAGCT
CTGGGAAGTCACTGCCAGGTATGTGAAGGGGATGGTCCATCTCTTCTACCAGAGTGATGATATTGTGAGG
GGAGACCCAGAGCTGCAGGCCTGGTGTCGGGAGATCACTGAAGTGGGGCTGTGCCATGCCCAGGACAGAG
GATTCCCTGTCTCCTTCCAGTCCCGGGCTCAGCTCTGCCATTTCCTCACCATGTGTGTTTTCACATGCAC
TGCCCAGCATGCTGCCATCAACCAGGGCCAGCTGGACTGGTATGGTTGGGTACCTAATGCTCCATGCACA
ATGAGGATGCCTCCGCCCACCAGCAAGGACGACGTGACGATGGAGACCGTGATGGGGTCGCTACCTGATG
TGCAGAAGGCCTGTCTTCAGATGACTATCACATGGCACCTGGGTCGCCTGCAGCCAGACATGGTACCTCT
AGGACACCACACAGAAAAATATTTCTCAGACCCAAGGACCAAAGCTGTGCTCAGCCAATTTCAAGCAGAT
TTGGACAATTTGGAGAAAGAGATTACAGCCCGGAATGAGCAACTTGACCTGCCTTATGAATACCTCAAGC
CCAGCCGCATAGAGAACAGTATCACCATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR227302 representing NM_007440
Red=Cloning site Green=Tags(s)

MGRYRVRVVTGAWLFSGSLNLVRLWLVGEHREAKLELQLRPARGKEEEFDFDVPEDLGPLQFVKLHKQHT
VVDDAWFCNLITVQGPGTSAEAVFPCYRWVQGEGILSLPEGTARLAGDNALDVFQKYREKELKERQQTYC
WATWKEGLPQTIAADCKDDLPPNMRFHEEKRLDFEWTLKAGVLEMGLKRVYTLLRSWNHLEDFDQIFWGQ
KSALAEKVHQCWQEDELFGYQFLNGANPMLLRRSTSLPSRLVLPSGMEELQAQLEKELKNGSLFEADFIL
LDGIPANVIRGEPQYLAAPLVMLRMDPGGKLLPMAIQIQPPNPSSPAPTLFLPSDPPLAWLLAKIWVRNS
DFQLQELQFHLLNTHLVAEVIAVATMRCLPGLHPIFKLLVPHIRYTMEINTRSRTQLISDGGIFDQVVST
GGGGHVQLLTRAVAQLTYHSLCPPDDLANRGLLRIPSALYARDALQLWEVTARYVKGMVHLFYQSDDIVR
GDPELQAWCREITEVGLCHAQDRGFPVSFQSRAQLCHFLTMCVFTCTAQHAAINQGQLDWYGWVPNAPCT
MRMPPPTSKDDVTMETVMGSLPDVQKACLQMTITWHLGRLQPDMVPLGHHTEKYFSDPRTKAVLSQFQAD
LDNLEKEITARNEQLDLPYEYLKPSRIENSITI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_007440
ORF Size 1989 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007440.2, NM_007440.3, NM_007440.4, NM_007440.5, NP_031466.2
RefSeq Size 2991 bp
RefSeq ORF 1992 bp
Locus ID 11684
Cytogenetics 11 42.99 cM
MW 75.8 kDa

Citations (1)

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