Ezh2 (NM_001146689) Mouse Tagged ORF Clone

CAT#: MR227124

Ezh2 (Myc-DDK-tagged) - Mouse enhancer of zeste homolog 2 (Drosophila) (Ezh2), transcript variant 2

Product Images

Circular map for MR227124

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ezh2
• Synonyms: Enx-1; Enx1h; KMT6; mKIAA4065
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227124 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCAGACTGGGAAGAAATCTGAGAAGGGACCGGTTTGTTGGCGGAAGCGTGTAAAATCAGAGTACA
  TGAGACTGAGACAGCTCAAGAGGTTCAGAAGAGCTGATGAAGTAAAGACTATGTTTAGTTCCAATCGTCA
  GAAAATTTTGGAAAGAACTGAAACCTTAAACCAAGAGTGGAAGCAGCGGAGGATACAGCCTGTGCACATC
  ATGACTTCTTGTTCAGTCACCAGTGACTTGGATTTTCCAGCACAAGTCATCCCGTTAAAGACCCTGAATG
  CAGTCGCCTCGGTGCCTATAATGTACTCTTGGTCGCCCTTACAACAGAATTTTATGGTGGAAGACGAAAC
  TGTTTTACATAACATTCCTTATATGGGGGATGAAGTTCTGGATCAGGATGGCACTTTCATTGAAGAACTA
  ATAAAAAATTATGATGGAAAAGTGCATGGTGACAGAGAATGTGGATTTATAAATGATGAAATTTTTGTGG
  AGTTGGTAAATGCTCTTGGTCAATATAATGATGATGATGATGACGATGATGGAGATGATCCAGATGAAAG
  AGAAGAAAAACAGAAAGATCTAGAGGATAATCGAGATGATAAAGAAACTTGCCCACCTCGGAAATTTCCT
  GCTGATAAAATATTTGAAGCCATTTCCTCAATGTTTCCAGATAAGGGCACCGCAGAAGAACTGAAAGAAA
  AATATAAAGAACTCACGGAGCAGCAGCTCCCAGGTGCTCTGCCTCCTGAATGTACTCCAAACATCGATGG
  ACCAAATGCCAAATCTGTTCAGAGGGAGCAAAGCTTGCATTCATTTCATACGCTCTTCTGTCGACGATGT
  TTTAAGTATGACTGCTTCCTACATCGTAAGTGCAGTTATTCCTTCCATGCAACACCCAACACATATAAGA
  GGAAGAACACAGAAACAGCTTTGGACAACAAGCCTTGTGGACCACAGTGTTACCAGCATCTGGAGGGAGC
  TAAGGAGTTTGCTGCTGCTCTTACTGCTGAGCGTATAAAGACACCACCTAAACGCCCAGGGGGCCGCAGA
  AGAGGAAGACTTCCGAATAACAGTAGCAGACCCAGCACCCCCACCATCAGTGTGCTGGAGTCAAAGGATA
  CAGACAGTGACAGAGAAGCAGGGACTGAAACTGGGGGAGAGAACAATGATAAAGAAGAAGAAGAGAAAAA
  AGATGAGACGTCCAGCTCCTCTGAAGCAAATTCTCGGTGTCAAACACCAATAAAGATGAAGCCAAATATT
  GAACCTCCTGAGAATGTGGAGTGGAGTGGTGCTGAAGCCTCCATGTTTAGAGTCCTCATTGGTACTTACT
  ACGATAACTTTTGTGCCATTGCTAGGCTAATTGGGACCAAAACATGTAGACAGGTGTATGAGTTTAGAGT
  CAAGGAGTCCAGTATCATAGCACCTGTTCCCACTGAGGATGTAGACACTCCTCCAAGAAAGAAGAAAAGG
  AAACATCGGTTGTGGGCTGCACACTGCAGAAAGATACAACTGAAAAAGGACGGCTCCTCTAACCATGTTT
  ACAACTATCAACCCTGTGACCATCCACGGCAGCCTTGTGACAGTTCGTGCCCTTGTGTGATAGCACAAAA
  TTTTTGTGAAAAGTTTTGTCAATGTAGTTCAGAGTGTCAAAACCGCTTTCCTGGATGTCGGTGCAAAGCA
  CAATGCAACACCAAACAGTGTCCATGCTACCTGGCTGTCCGAGAGTGTGACCCTGACCTCTGTCTCACGT
  GTGGAGCTGCTGACCATTGGGACAGTAAAAATGTATCCTGTAAGAACTGTAGCATTCAGCGGGGCTCTAA
  AAAGCACTTACTGCTGGCACCGTCTGATGTGGCAGGCTGGGGCATCTTTATCAAAGATCCTGTACAGAAA
  AATGAATTCATCTCAGAATACTGTGGGGAGATTATTTCTCAGGATGAAGCAGACAGAAGAGGAAAAGTGT
  ATGACAAATACATGTGCAGCTTTCTGTTCAACTTGAACAATGATTTTGTGGTGGATGCAACCCGAAAGGG
  CAACAAAATTCGTTTTGCTAATCATTCAGTAAATCCAAACTGCTATGCAAAAGTTATGATGGTTAATGGT
  GACCACAGGATAGGCATCTTTGCTAAGAGGGCTATCCAGACTGGTGAAGAGTTGTTTTTTGATTACAGAT
  ACAGCCAGGCTGATGCCCTGAAGTATGTGGGCATCGAACGAGAAATGGAAATCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227124 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKTMFSSNRQKILERTETLNQEWKQRRIQPVHI
  MTSCSVTSDLDFPAQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEEL
  IKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPDEREEKQKDLEDNRDDKETCPPRKFP
  ADKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRC
  FKYDCFLHRKCSYSFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRR
  RGRLPNNSSRPSTPTISVLESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNI
  EPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPVPTEDVDTPPRKKKR
  KHRLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKA
  QCNTKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQK
  NEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNG
  DHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146689
• ORF Size: 2226 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146689.1, NP_001140161.1
• RefSeq Size: 2653 bp
• RefSeq ORF: 2229 bp
• Locus ID: 14056
• Cytogenetics: 6 22.92 cM
• MW: 84.9 kDa
• Gene Summary: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.[UniProtKB/Swiss-Prot Function]