Cd34 (NM_001111059) Mouse Tagged ORF Clone

CAT#: MR227078

Cd34 (Myc-DDK-tagged) - Mouse CD34 antigen (Cd34), transcript variant 1

Product Images

Circular map for MR227078

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cd34
• Synonyms: AU040960
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR227078 representing NM_001111059
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGTCCACAGGGACACGCGCGCGGGGCTCCTGCTGCCATGGCGCTGGGTAGCTCTCTGCCTGATGA
  GTCTGCTGCATCTAAATAACTTGACTTCTGCTACCACGGAGACTTCTACACAAGGAATATCCCCATCAGT
  TCCTACCAATGAGTCTGTTGAGGAAAATATCACATCTAGCATCCCTGGAAGTACCAGCCACTACTTGATC
  TATCAGGACAGCAGTAAGACCACACCAGCCATCTCAGAGACTATGGTCAACTTTACAGTTACCTCTGGGA
  TCCCTTCAGGCTCTGGAACTCCACACACTTTTTCACAACCACAGACTTCCCCAACTGGCATACTGCCTAC
  TACTTCAGACAGTATTTCCACTTCAGAGATGACCTGGAAGTCCAGCCTGCCATCTATAAATGTTTCTGAT
  TATTCGCCTAATAATAGCAGCTTTGAGATGACATCACCCACCGAGCCATATGCTTACACATCATCTTCTG
  CTCCGAGTGCCATTAAGGGAGAAATCAAATGCTCTGGAATCCGAGAAGTGAGGTTGGCCCAGGGTATCTG
  CCTGGAACTAAGTGAAGCATCTAGTTGTGAGGAGTTTAAGAAGGAAAAGGGAGAAGATCTAATTCAAATA
  CTGTGTGAAAAGGAGGAGGCTGAGGCTGATGCTGGTGCTAGTGTCTGCTCCCTGCTTCTAGCCCAGTCTG
  AGGTTAGGCCTGAGTGTTTGCTGATGGTCTTGGCCAATAGCACAGAACTTCCCAGCAAACTCCAGCTTAT
  GGAAAAGCACCAATCTGACTTGAGAAAGCTGGGGATCCAAAGCTTCAATAAACAAGATATCGGGAGCCAC
  CAGAGCTATTCCCGAAAGACTCTTATTGCATTGGTCACCTCTGGAGTTCTGCTGGCCATCTTGGGCACCA
  CTGGTTATTTCCTGATGAACCGTCGCAGTTGGAGCCCTACAGGAGAAAGGCTGGAGCTGGAACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR227078 representing NM_001111059
  Red=Cloning site Green=Tags(s)
  
  MQVHRDTRAGLLLPWRWVALCLMSLLHLNNLTSATTETSTQGISPSVPTNESVEENITSSIPGSTSHYLI
  YQDSSKTTPAISETMVNFTVTSGIPSGSGTPHTFSQPQTSPTGILPTTSDSISTSEMTWKSSLPSINVSD
  YSPNNSSFEMTSPTEPYAYTSSSAPSAIKGEIKCSGIREVRLAQGICLELSEASSCEEFKKEKGEDLIQI
  LCEKEEAEADAGASVCSLLLAQSEVRPECLLMVLANSTELPSKLQLMEKHQSDLRKLGIQSFNKQDIGSH
  QSYSRKTLIALVTSGVLLAILGTTGYFLMNRRSWSPTGERLELEP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001111059
• ORF Size: 975 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001111059.1, NP_001104529.1
• RefSeq Size: 2621 bp
• RefSeq ORF: 978 bp
• Locus ID: 12490
• Cytogenetics: 1 98.38 cM
• MW: 35.7 kDa
• Gene Summary: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins (By similarity).[UniProtKB/Swiss-Prot Function]