Jak1 (NM_146145) Mouse Tagged ORF Clone

CAT#: MR226924

Jak1 (Myc-DDK-tagged) - Mouse Janus kinase 1 (Jak1)

Product Images

Circular map for MR226924

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Jak1
• Synonyms: AA960307; BAP004; C130039L05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226924 representing NM_146145
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTATCTAAATATAAAAGAGGACTGCAATGCCATGGCGTTCTGTGCTAAAATGAGGAGCTTCAAGA
  AGACTGAGGTGAAGCAGGTGGTCCCTGAGCCTGGAGTGGAGGTGACTTTCTATCTGTTGGACAGGGAGCC
  CCTCCGCCTGGGCAGCGGAGAGTATACAGCCGAGGAGCTGTGCATCAGGGCCGCCCAGGAGTGCAGTATC
  TCTCCTCTCTGTCACAACCTCTTCGCCCTGTACGATGAGAGCACCAAGCTCTGGTACGCTCCGAACCGAA
  TCATCACTGTGGATGACAAAACGTCTCTCCGGCTCCACTACCGCATGAGGTTCTACTTTACCAACTGGCA
  CGGAACCAATGACAACGAACAGTCTGTATGGCGACATTCTCCAAAGAAGCAGAAAAACGGCTATGAGAAG
  AAAAGGGTTCCAGAAGCAACCCCACTCCTTGATGCCAGTTCACTGGAGTATCTGTTTGCACAGGGACAGT
  ATGATTTGATCAAATGCCTGGCTCCCATTCGGGACCCCAAGACGGAGCAAGACGGACATGATATTGAAAA
  TGAGTGCCTGGGCATGGCGGTCCTGGCCATCTCCCACTATGCCATGATGAAGAAGATGCAGTTGCCGGAA
  CTTCCCAAAGACATCAGCTACAAGCGATATATTCCAGAAACATTGAATAAATCCATCAGACAGAGGAACC
  TTCTTACCAGGATGCGAATAAATAATGTTTTCAAGGATTTCTTGAAGGAATTTAACAACAAGACCATCTG
  TGACAGCAGTGTGTCTACACATGACCTGAAGGTGAAATACCTGGCTACCTTGGAAACTTTGACAAAACAT
  TATGGAGCTGAAATATTTGAGACTTCTATGCTACTGATTTCATCAGAAAATGAATTGAGTCGATGCCATT
  CGAATGACAGTGGCAATGTTCTCTATGAGGTCATGGTGACTGGAAATCTCGGGATCCAGTGGCGGCAGAA
  ACCAAATGTTGTTCCTGTTGAAAAGGAAAAAAATAAACTGAAGCGGAAAAAACTGGAATATAATAAACAC
  AAGAAGGATGATGAGAGAAACAAACTCCGGGAAGAGTGGAACAATTTTTCCTATTTCCCTGAAATCACCC
  ACATTGTAATAAAGGAGTCTGTGGTCAGCATTAACAAACAGGACAACAAAAACATGGAACTCAAGCTCTC
  TTCTCGAGAGGAAGCCTTGTCCTTTGTGTCCCTGGTGGATGGCTACTTCCGGCTCACTGCAGATGCCCAC
  CATTACCTCTGTACTGATGTGGCTCCCCCACTGATTGTCCACAATATACAGAACGGCTGCCACGGTCCAA
  TCTGCACAGAATATGCCATCAATAAGCTGCGGCAGGAAGGGAGTGAAGAGGGGATGTACGTGCTGAGGTG
  GAGCTGCACCGACTTTGACAACATTCTTATGACTGTCACCTGCTTTGAAAAGTCTGAGGTATTGGGTGGC
  CAGAAGCAGTTCAAGAACTTTCAGATTGAGGTACAGAAGGGCCGCTACAGCCTGCATGGCTCTATGGACC
  ACTTTCCCAGCCTGCGAGACCTCATGAACCACCTCAAGAAGCAGATCCTGCGCACGGACAACATAAGCTT
  TGTGCTGAAACGATGCTGTCAGCCTAAGCCTCGAGAAATCTCCAATCTGCTCGTAGCCACTAAGAAAGCC
  CAGGAGTGGCAGCCTGTCTACTCCATGAGCCAGCTGAGCTTTGATCGGATCCTTAAGAAAGATATTATAC
  AAGGTGAGCACCTTGGCAGAGGCACAAGAACACATATCTATTCTGGGACCCTGCTGGACTACAAGGATGA
  GGAAGGAATTGCTGAAGAGAAGAAGATAAAAGTGATCCTCAAAGTCCTAGACCCCAGCCACCGGGACATC
  TCTCTGGCCTTCTTTGAGGCTGCTAGCATGATGAGACAGGTTTCCCACAAACATATAGTGTACCTCTACG
  GCGTGTGTGTCCGAGATGTGGAAAATATCATGGTGGAAGAGTTTGTGGAGGGGGGGCCGTTGGATCTCTT
  CATGCACCGGAAAAGTGATGCGCTTACTACCCCCTGGAAGTTCAAGGTTGCCAAACAGCTGGCCAGTGCC
  CTGAGTTACTTGGAAGATAAAGACCTGGTTCATGGAAATGTGTGCACTAAAAACCTCCTTCTGGCCCGTG
  AGGGCATTGACAGTGACATTGGCCCGTTCATCAAGCTTAGTGACCCTGGCATCCCAGTCTCTGTGCTGAC
  CAGGCAAGAGTGCATAGAGCGAATCCCCTGGATCGCTCCTGAGTGTGTTGAAGACTCCAAGAACCTGAGT
  GTGGCTGCTGACAAGTGGAGCTTTGGAACCACGCTCTGGGAAATCTGCTACAACGGAGAGATTCCTCTCA
  AAGACAAGACCCTCATTGAGAAAGAGAGGTTTTATGAAAGCCGCTGCAGGCCTGTGACTCCATCTTGCAA
  GGAGCTAGCTGACCTCATGACTCGCTGCATGAACTATGACCCCAACCAGAGACCCTTCTTCCGAGCCATC
  ATGAGGGACATTAACAAGCTGGAGGAGCAGAATCCAGACATTGTTTCAGAAAAGCAGCCAACAACAGAGG
  TGGACCCCACTCACTTTGAAAAGCGGTTCCTGAAGAGGATTCGTGACTTGGGAGAGGGTCACTTTGGGAA
  GGTTGAGCTCTGCAGATATGATCCTGAGGGAGACAACACAGGGGAGCAGGTAGCTGTCAAGTCCCTGAAG
  CCTGAGAGTGGAGGTAACCACATAGCTGATCTGAAGAAGGAGATAGAGATCTTACGGAACCTCTACCATG
  AGAACATTGTGAAGTACAAAGGAATCTGCATGGAAGACGGAGGCAATGGTATCAAGCTCATCATGGAGTT
  TCTGCCTTCGGGAAGCCTAAAGGAGTATCTGCCAAAGAATAAGAACAAAATCAACCTCAAACAGCAGCTA
  AAATATGCCATCCAGATTTGTAAGGGGATGGACTACTTGGGTTCTCGGCAATACGTTCACCGGGACTTAG
  CAGCAAGAAATGTCCTTGTTGAGAGTGAGCATCAAGTGAAGATCGGAGACTTTGGTTTAACCAAAGCAAT
  TGAAACCGATAAGGAGTACTACACAGTCAAGGACGACCGGGACAGCCCAGTGTTCTGGTACGCTCCGGAA
  TGTTTAATCCAGTGTAAATTTTATATCGCCTCTGATGTCTGGTCTTTTGGAGTGACACTGCACGAGCTGC
  TCACTTACTGTGACTCAGATTTTAGTCCCATGGCCTTGTTCCTGAAAATGATAGGCCCAACTCATGGCCA
  GATGACAGTGACACGGCTTGTGAATACTCTGAAAGAAGGAAAGCGTCTGCCATGTCCACCCAACTGTCCT
  GATGAGGTTTATCAGCTTATGAGAAAATGCTGGGAATTCCAACCATCTAACCGGACAACTTTTCAGAACC
  TTATTGAAGGATTTGAAGCACTTTTAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226924 representing NM_146145
  Red=Cloning site Green=Tags(s)
  
  MQYLNIKEDCNAMAFCAKMRSFKKTEVKQVVPEPGVEVTFYLLDREPLRLGSGEYTAEELCIRAAQECSI
  SPLCHNLFALYDESTKLWYAPNRIITVDDKTSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEK
  KRVPEATPLLDASSLEYLFAQGQYDLIKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPE
  LPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKH
  YGAEIFETSMLLISSENELSRCHSNDSGNVLYEVMVTGNLGIQWRQKPNVVPVEKEKNKLKRKKLEYNKH
  KKDDERNKLREEWNNFSYFPEITHIVIKESVVSINKQDNKNMELKLSSREEALSFVSLVDGYFRLTADAH
  HYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEVLGG
  QKQFKNFQIEVQKGRYSLHGSMDHFPSLRDLMNHLKKQILRTDNISFVLKRCCQPKPREISNLLVATKKA
  QEWQPVYSMSQLSFDRILKKDIIQGEHLGRGTRTHIYSGTLLDYKDEEGIAEEKKIKVILKVLDPSHRDI
  SLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDALTTPWKFKVAKQLASA
  LSYLEDKDLVHGNVCTKNLLLAREGIDSDIGPFIKLSDPGIPVSVLTRQECIERIPWIAPECVEDSKNLS
  VAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAI
  MRDINKLEEQNPDIVSEKQPTTEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLK
  PESGGNHIADLKKEIEILRNLYHENIVKYKGICMEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQL
  KYAIQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPE
  CLIQCKFYIASDVWSFGVTLHELLTYCDSDFSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCP
  DEVYQLMRKCWEFQPSNRTTFQNLIEGFEALLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146145
• ORF Size: 3459 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146145.2, NP_666257.2
• RefSeq Size: 5299 bp
• RefSeq ORF: 3462 bp
• Locus ID: 16451
• Cytogenetics: 4 46.19 cM
• MW: 133.8 kDa