Usp32 (NM_001029934) Mouse Tagged ORF Clone

CAT#: MR226778

Usp32 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 32 (Usp32)

Product Images

Circular map for MR226778

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Usp32
• Synonyms: 2900074J03Rik; 6430526O11Rik; AW045245
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226778 representing NM_001029934
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCCAAGGAGTCACGGATCGGATTCCTCAGCTACGAGGAGGCGCTGAGGAGAGTTACAGATGTTG
  AGCTGAAACGACTCAAAGATGCCTTCAAGAGGACCTGCGGCCTCTCCTATTACATGAGTCAGCACTGCTT
  CATCAGGGAAGTGCTTGGGGATGGAGTACCTCCTAAAGTTGCTGAGGTGATTTACTGTTCTTTTGGTGGA
  ACATCCAAAGGGCTGCATTTCAATAACTTAATAGTTGGACTCGTCCTCCTTACAAGAGGCAAAGATGAAG
  AGAAAGCAAAATATATTTTTAGTCTTTTTTCAAGTGAATCCGGGAGCTATGTTGTACGGGAAGAAATGGA
  GAGAATGCTCCATGTGGTGGATGGTAAAGTCCCAGATACACTCAGGAAGTGTTTCTCAGAGGGTGAAAAG
  GTAAACTATGAAAAATTCAGAAATTGGCTTCTTCTAAACAAAGATGCTTTTACCTTCTCTCGCTGGCTGT
  TGTCTGGGGGTGTATATGTGACCCTCACTGATGACAGTGACACTCCTACTTTCTACCAAACTCTGGCTGG
  AGTCACACATTTGGAAGAGTCAGACATCATCGATCTGGAGAAACGGTATTGGCTACTGAAGGCCCAATCC
  CGAACTGGACGTTTTGACTTAGAGACCTTTGGTCCATTGGTTTCACCACCTATTCGCCCTTCTCTGAGTG
  AAGGATTATTTAATGCTTTTGATGAAAATCGTGACAATCACATAGACTTCAAGGAGATATCCTGTGGGTT
  ATCAGCCTGCTGCAGGGGCCCCCTGGCTGAAAGACAAAAATTTTGCTTCAAGGTGTTTGATGTTGACCGT
  GATGGAGTTCTCTCCAGGGTTGAACTGAAAGACATGGTAGTTGCACTTTTGGAGGTCTGGAAAGATAACC
  GCACTGATGATATTCCTGAATTGCATATGGATTTGTCAGACATTGTAGAAAGGATATTGAATGCACATGA
  TACCACAAAGGTGGGCCATCTTACTCTTGAAGACTATCAGATATGGAGTGTGAAAAATGTTCTTGCCAAT
  GAGTTTTTGAATCTCCTCTTCCAGGTATGCCACATAGTTCTGGGGTTAAGACCAGCTACTCCAGAAGAAG
  AAGGACAAATTATTAGAGGATGGTTGGAACGAGAAAGCAGGTATGGTCTGCAGCCAGGACACAATTGGTT
  TATCATCTCCATGCAGTGGTGGCAACAGTGGAAAGAATATGTCAAATATGATGCCAGCCCTGTTGTAATT
  GAGCCATCATCTGTTCTAAATGGAGGAAAATTTTCATTTGGAACAGCAGCACATCCTATAGAGCATGGAG
  AAGATAGAATCAGTAATAACCTTGGTTATATGAATACCACAGAAGAGAAATACTCAGACAACATTTCTTC
  TGCATCTGAAGCCTCAGAGTCTACTGGCAGTGGCTTTCTGTATTCTGGCACACCAGGGGCAGATATGTGC
  TTTGCAAGGCAGCATAACACTTCTGACAATAACAACCAGTGTTTACTAGGAGCCAATGGGAATATCCTTT
  TGCACCTTAATCCTCAGAAGCCAGGAGCAATTGATAACCAGCCACTAGTAACTCAGGAACCAGTAAAGGC
  TACATCATTAACACTAGAAGGAGGGCGATTAAAACGAACTCCACAGCTAATCCATGGAAGAGATTATGAA
  ATGGTCCCAGAACCCGTGTGGAGAGCACTTTATCATTGGTATGGATCAAACCTGGCCCTTCCTAGACCAG
  TAATCAAGAATAGCAAAACGGACATCCCAGAACTAGAATTATTTCCTCGCTATCTTCTCTTCCTTCGACA
  GCAGCCTGCCACACGGACACAGCAATCTAACATCTGGGTGAATATGGGAAATGTACCCTCTCCAAATGCG
  CCTCTAAAGCGAGTGTTAGCCTATACAGGCTGTTTTAGTAGAATGCAAACCATCAAGGAAATTCATGAAT
  ATCTGTCTCAAAGGCTTCGCATTAAAGAGGAAGATATGCGGCTGTGGCTGTACAATAGTGAGAACTACCT
  GACTCTTCTGGATGATGAGGATCACAGATTGGAATATTTGAAAATCCAGGATGAGCAGCACCTGGTAATT
  GAAGTTCGAAACAAAGATATGAGTTGGCCTGAAGAAATGTCTTTTATAGCAAACAGTAGCAAAATAGATA
  GACATAAAGTTCCCACAGAAAAGGGCGCCACAGGTCTGAGTAACCTGGGAAACACATGCTTCATGAACTC
  AAGCATCCAGTGTGTCAGCAATACACAACCACTGACACAGTACTTTATCTCAGGGAGGCATCTTTATGAA
  CTCAACAGGACAAATCCCATTGGTATGAAAGGCCATATGGCTAAATGTTATGGCGATTTGGTGCAAGAAC
  TTTGGAGTGGAACTCAGAAGAATGTTGCCCCCTTAAAGCTCCGGTGGACCATAGCAAAATATGCTCCCAG
  GTTTAATGGATTCCAGCAACAAGACTCCCAAGAACTTCTGGCTTTCCTCTTGGATGGTCTTCATGAAGAT
  CTCAACCGAGTCCACGAGAAACCATATGTAGAACTAAAAGACAGTGACGGCCGACCCGACTGGGAAGTAG
  CTGCAGAGGCCTGGGACAACCATCTAAGGAGAAATAGATCAATTGTTGTGGACTTGTTCCATGGTCAGCT
  AAGATCCCAAGTCAAATGCAAGACATGTGGGCATATAAGTGTCCGATTCGACCCTTTTAATTTTTTGTCT
  TTACCACTACCAATGGACAGTTACATGCATTTAGAAATAACAGTAATTAAATTAGATGGTACTACCCCCA
  TACGATATGGACTAAGACTGAATATGGATGAAAAGTATACAGGTTTGAAAAAGCAGCTGAGTGATCTCTG
  TGGACTTAAATCAGAACAAATCCTTTTTGCAGAAGTGCATAGTTCAAACATAAAGAACTTCCCTCAGGAC
  AACCAAAAAGTTCGACTCTCAGTGAGTGGATTTTTGTGTGCATTTGAAATTCCTATTCCTGCATCTCCAG
  TTTCAGCTTGCAGTCCAATTCAAACAGATTGCTCTTCTTCTCCATCTACAAATGGACTGTTTACCTTGAC
  TACCAATGGGGATCTGCCTCGACCTATATTCATCCCCAATGGAATGCCAAATACAGTTGTACCATGTGGA
  ACTGAGAAGAATGTCACAAATGGAATAGTGAATGGTCATATGCCGCCTCTTCCTGATGACCCCTTTACAG
  GCTACATCATTGCAGTCCACCGAAAAATGATGAGGACAGAACTGTATTTCCTGTCATCTCAGAAGAATCG
  CCCCAGTCTCTTTGGGATGCCACTGATTGTTCCATGTACTGTACATACCCGGAAAAAAGATCTCTATGAT
  GCAGTTTGGATTCAAGTATCTCGATTAGCCAGTCCGCTCCCACCTCAAGAAGCTAGCAACCATGCACAGG
  ATTGTGATGACAGCATGGGCTATCAATATCCATTCACTCTCCGAGTTGTGCAGAAAGATGGAAACTCCTG
  TGCCTGGTGTCCATGGTATAGATTTTGCAGAGGCTGTAAAATCGATTGTGGAGAAGACAGAGCTTTCATT
  GGAAATGCCTGTATTGCTGTGGATTGGGACCCTACAGCCCTTCACCTCCGCTATCAAACATCACAGGAAA
  GAGTTGTAGAGGAGCATGAAAGTGTGGAACAGAGTCGGCGAGCACAAGCTGAGCCCATCAACCTGGACAG
  CTGTCTCCGTGCCTTCACCAGTGAGGAGGAACTGGGGGAAAATGAGATGTACTACTGCTCCAAATGTAAA
  ACACACTGTTTGGCAACCAAGAAACTGGACCTCTGGAGGCTTCCTCCAATCCTGATTATTCACCTTAAAC
  GATTTCAGTTTGTAAATGGACGGTGGATAAAATCACAGAAAATTGTTAAATTTCCTCGTGAAAGTTTTGA
  CCCTAGTGCTTTTTTAGTACCAAGAGACCCAACTCTTTGCCAGCATAAACCACTCACACCCCAGGGGGAT
  GACTTCTCTGAGCTAAGGATCCCAGCAGGAGATGTGAAGAAAGTGGATATCCAGAGCTCAGCAGGGGAGG
  AGGATGTGCTCCTGAGCAAGAGCCCATCTTCACTCAGTGCAAATGTCACCAGTAGCCCAAAAGGTTCACC
  TTCTTCATCAAGAAAAAGTGGAGCCAGCTGTCCCTCCAGCAAAAATAGCAGCCCTAACAGCAGCCCACGG
  ACCTTGGGGAGGAACAAAGGGAGGCTCCGGCTGCCTCAAATTGGCAGCAAAAATAAATTGTCCAATAGTA
  AGGAGAATTTGGATACCAGCAAAGAGAATGGGGCTGGGCAGATCTGTGAACTGGCTGACACCTTGAACCG
  AAGACATGTATTGGGAGGCAGCCAGCCTGAGCTGGTCACTCCTCTGGATCATGAGATCACTTTGGCTAAT
  GGATTTCTTTATGAGCATGAGGCATGTGGCAATGGCTATAGCAATGGTCAGCTTGGAAACCATAGTGAAG
  AAGACAGCACTGATGACCAAAGAGAAGAAACACACAGTAAACCTATTTATAATCTATATGCAATTTCGTG
  CCATTCAGGAATTCTGGGTGGGGGCCATTACGTCACTTATGCCAAAAACCCAAACTGCAAGTGGTACTGT
  TACAATGACAGCAGCTGTAAGGAGCTTCACCCTGATGAGATAGACACCGACTCTGCCTACATTCTTTTCT
  ATGAGCAGCAGGGGATAGACTGTGCACAGTTTCTGCCAAAGACAGACGGCAAGAAGATGGCGGACACGAG
  CAGCATGGACGAAGACTTTGAGTCTGACTACAAGAAGTACTGTGTGCTACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226778 representing NM_001029934
  Red=Cloning site Green=Tags(s)
  
  MGAKESRIGFLSYEEALRRVTDVELKRLKDAFKRTCGLSYYMSQHCFIREVLGDGVPPKVAEVIYCSFGG
  TSKGLHFNNLIVGLVLLTRGKDEEKAKYIFSLFSSESGSYVVREEMERMLHVVDGKVPDTLRKCFSEGEK
  VNYEKFRNWLLLNKDAFTFSRWLLSGGVYVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQS
  RTGRFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDR
  DGVLSRVELKDMVVALLEVWKDNRTDDIPELHMDLSDIVERILNAHDTTKVGHLTLEDYQIWSVKNVLAN
  EFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQPGHNWFIISMQWWQQWKEYVKYDASPVVI
  EPSSVLNGGKFSFGTAAHPIEHGEDRISNNLGYMNTTEEKYSDNISSASEASESTGSGFLYSGTPGADMC
  FARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYE
  MVPEPVWRALYHWYGSNLALPRPVIKNSKTDIPELELFPRYLLFLRQQPATRTQQSNIWVNMGNVPSPNA
  PLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHRLEYLKIQDEQHLVI
  EVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYE
  LNRTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHED
  LNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLS
  LPLPMDSYMHLEITVIKLDGTTPIRYGLRLNMDEKYTGLKKQLSDLCGLKSEQILFAEVHSSNIKNFPQD
  NQKVRLSVSGFLCAFEIPIPASPVSACSPIQTDCSSSPSTNGLFTLTTNGDLPRPIFIPNGMPNTVVPCG
  TEKNVTNGIVNGHMPPLPDDPFTGYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTRKKDLYD
  AVWIQVSRLASPLPPQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDRAFI
  GNACIAVDWDPTALHLRYQTSQERVVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCK
  THCLATKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLVPRDPTLCQHKPLTPQGD
  DFSELRIPAGDVKKVDIQSSAGEEDVLLSKSPSSLSANVTSSPKGSPSSSRKSGASCPSSKNSSPNSSPR
  TLGRNKGRLRLPQIGSKNKLSNSKENLDTSKENGAGQICELADTLNRRHVLGGSQPELVTPLDHEITLAN
  GFLYEHEACGNGYSNGQLGNHSEEDSTDDQREETHSKPIYNLYAISCHSGILGGGHYVTYAKNPNCKWYC
  YNDSSCKELHPDEIDTDSAYILFYEQQGIDCAQFLPKTDGKKMADTSSMDEDFESDYKKYCVLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001029934
• ORF Size: 4812 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001029934.1, NP_001025105.1
• RefSeq Size: 6978 bp
• RefSeq ORF: 4815 bp
• Locus ID: 237898
• Cytogenetics: 11 C
• MW: 181.7 kDa