Ar (NM_013476) Mouse Tagged ORF Clone

CAT#: MR226737

Ar (Myc-DDK-tagged) - Mouse androgen receptor (Ar)

Product Images

Circular map for MR226737

• 

  Western blot validation of overexpressed Ar protein using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MR226737 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ar
• Synonyms: AW320017; Tfm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226737 representing NM_013476
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGCAGTTAGGGCTGGGAAGGGTCTACCCACGGCCCCCATCCAAGACCTATCGAGGAGCGTTCC
  AGAATCTGTTCCAGAGCGTGCGCGAAGCGATCCAGAACCCGGGCCCCAGGCACCCTGAGGCCGCTAACAT
  AGCACCTCCCGGCGCCTGTTTACAGCAGAGGCAGGAGACTAGCCCCCGGCGGCGGCGGCGGCAGCAGCAC
  ACTGAGGATGGTTCTCCTCAAGCCCACATCAGAGGCCCCACAGGCTACCTGGCCCTGGAGGAGGAACAGC
  AGCCTTCACAGCAGCAGGCAGCCTCCGAGGGCCACCCTGAGAGCAGCTGCCTCCCCGAGCCTGGGGCGGC
  CACCGCTCCTGGCAAGGGGCTGCCGCAGCAGCCACCAGCTCCTCCAGATCAGGATGACTCAGCTGCCCCA
  TCCACGTTGTCCCTGCTGGGCCCCACTTTCCCAGGCTTAAGCAGCTGCTCCGCCGACATTAAAGACATTT
  TGAACGAGGCCGGCACCATGCAACTTCTTCAGCAGCAGCAACAACAGCAGCAGCACCAACAGCAGCACCA
  ACAGCACCAACAGCAGCAGGAGGTAATCTCCGAAGGCAGCAGCGCAAGAGCCAGGGAGGCCACGGGGGCT
  CCCTCTTCCTCCAAGGATAGTTACCTAGGGGGCAATTCAACCATATCTGACAGTGCCAAGGAGTTGTGTA
  AAGCAGTGTCTGTGTCCATGGGATTGGGTGTGGAAGCATTGGAACATCTGAGTCCAGGGGAACAGCTTCG
  GGGAGACTGCATGTACGCGTCGCTCCTGGGAGGTCCACCCGCGGTGCGTCCCACTCCTTGTGCGCCGCTG
  CCCGAATGCAAAGGTCTTCCCCTGGACGAAGGCCCAGGCAAAAGCACTGAAGAGACTGCTGAGTATTCCT
  CTTTCAAGGGAGGTTACGCCAAAGGATTGGAAGGTGAGAGCTTGGGGTGCTCTGGCAGCAGTGAAGCAGG
  TAGCTCTGGGACACTTGAGATCCCGTCCTCTCTGTCTCTGTATAAATCTGGAGCACTAGACGAGGCAGCA
  GCATACCAGAATCGCGACTACTACAACTTTCCGCTGGCTCTGTCCGGGCCGCCGCACCCCCCGCCCCCTA
  CCCATCCACACGCCCGTATCAAGCTGGAGAACCCATTGGACTACGGCAGCGCCTGGGCTGCGGCGGCAGC
  GCAATGCCGCTATGGGGACTTGGGTAGTCTACATGGAGGGAGTGTAGCCGGGCCCAGCACTGGATCGCCC
  CCAGCCACCACCTCTTCTTCCTGGCATACTCTCTTCACAGCTGAAGAAGGCCAATTATATGGGCCAGGAG
  GCGGGGGCGGCAGCAGCAGCCCAAGCGATGCCGGGCCTGTAGCCCCCTATGGCTACACTCGGCCCCCTCA
  GGGGCTGACAAGCCAGGAGAGTGACTACTCTGCCTCCGAAGTGTGGTATCCTGGTGGAGTTGTGAACAGA
  GTACCCTATCCCAGTCCCAATTGTGTCAAAAGTGAAATGGGACCTTGGATGGAGAACTACTCCGGACCTT
  ATGGGGACATGCGTTTGGACAGTACCAGGGACCATGTTTTACCCATCGACTATTACTTTCCACCCCAGAA
  GACCTGCCTGATCTGTGGAGATGAAGCTTCTGGCTGTCACTACGGAGCTCTCACTTGTGGCAGCTGCAAG
  GTCTTCTTCAAAAGAGCCGCTGAAGGGAAACAGAAGTATCTATGTGCCAGCAGAAACGATTGTACCATTG
  ATAAATTTCGGAGGAAAAATTGCCCATCTTGTCGTCTCCGGAAATGTTATGAAGCAGGGATGACTCTGGG
  AGCTCGTAAGCTGAAGAAACTTGGAAATCTAAAACTACAGGAGGAAGGAGAAAACTCCAATGCTGGCAGC
  CCCACTGAGGACCCATCCCAGAAGATGACTGTATCACACATTGAAGGCTATGAATGTCAGCCTATCTTTC
  TTAACGTCCTGGAAGCCATTGAGCCAGGAGTGGTGTGTGCCGGACATGACAACAACCAACCAGATTCCTT
  TGCTGCCTTGTTATCTAGCCTCAATGAGCTTGGAGAGAGGCAGCTTGTGCATGTGGTCAAGTGGGCCAAG
  GCCTTGCCTGGCTTCCGCAACTTGCATGTGGATGACCAGATGGCGGTCATTCAGTATTCCTGGATGGGAC
  TGATGGTATTTGCCATGGGTTGGCGGTCCTTCACTAATGTCAACTCCAGGATGCTCTACTTTGCACCTGA
  CTTGGTTTTCAATGAGTACCGCATGCACAAGTCTCGGATGTACAGCCAGTGTGTGAGGATGAGGCACCTG
  TCTCAAGAGTTTGGATGGCTCCAAATAACCCCCCAGGAATTCCTGTGCATGAAAGCACTGCTGCTCTTCA
  GCATTATTCCAGTGGATGGGCTGAAAAATCAAAAATTCTTTGATGAACTTCGAATGAACTACATCAAGGA
  ACTCGATCGCATCATTGCATGCAAAAGAAAGAATCCCACATCCTGCTCAAGGCGCTTCTACCAGCTCACC
  AAGCTCCTGGATTCTGTGCAGCCTATTGCAAGAGAGCTGCATCAGTTCACTTTTGACCTGCTAATCAAGT
  CCCATATGGTGAGCGTGGACTTTCCTGAAATGATGGCAGAGATCATCTCTGTGCAAGTGCCCAAGATCCT
  TTCTGGGAAAGTCAAGCCCATCTATTTCCACACACAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226737 representing NM_013476
  Red=Cloning site Green=Tags(s)
  
  MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREAIQNPGPRHPEAANIAPPGACLQQRQETSPRRRRRQQH
  TEDGSPQAHIRGPTGYLALEEEQQPSQQQAASEGHPESSCLPEPGAATAPGKGLPQQPPAPPDQDDSAAP
  STLSLLGPTFPGLSSCSADIKDILNEAGTMQLLQQQQQQQQHQQQHQQHQQQQEVISEGSSARAREATGA
  PSSSKDSYLGGNSTISDSAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYASLLGGPPAVRPTPCAPL
  PECKGLPLDEGPGKSTEETAEYSSFKGGYAKGLEGESLGCSGSSEAGSSGTLEIPSSLSLYKSGALDEAA
  AYQNRDYYNFPLALSGPPHPPPPTHPHARIKLENPLDYGSAWAAAAAQCRYGDLGSLHGGSVAGPSTGSP
  PATTSSSWHTLFTAEEGQLYGPGGGGGSSSPSDAGPVAPYGYTRPPQGLTSQESDYSASEVWYPGGVVNR
  VPYPSPNCVKSEMGPWMENYSGPYGDMRLDSTRDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCGSCK
  VFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGENSNAGS
  PTEDPSQKMTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAK
  ALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHL
  SQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLT
  KLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013476
• ORF Size: 2697 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013476.4
• RefSeq Size: 2999 bp
• RefSeq ORF: 2700 bp
• Locus ID: 11835
• UniProt ID: P19091
• Cytogenetics: X 42.82 cM
• MW: 98.6 kDa
• Gene Summary: This gene encodes a nuclear hormone receptor containing zinc finger and DNA-binding domains. The encoded protein is a key regulator of signalling by androgens, a class of steroid hormones involved in male reproductive development. The protein responds to hormone signalling by translocating to the nucleus, forming dimers, and binding to androgen response elements (AREs) in the promoters of target genes, which are subsequently transcriptionally activated. Activity of this protein is negatively regulated by nuclear receptor subfamily 0 group B member 1 (Nr0b1, also known as Dax1). Mutations in this gene result in feminized genitals and infertility in male animals. Loss of function in female animals also causes problems in reproductive development and function. [provided by RefSeq, May 2015]