Ret (NM_009050) Mouse Tagged ORF Clone

USD 1,170.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Ret (NM_009050) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Ret
Synonyms c-Ret; PTC; RET9; RET51
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR226724 representing NM_009050
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGAAAGCGACGTCCGGCGCCGCAGGGCTGGGGCTGAAGCTGATTTTGCTCCTGCCGCTGCTAGGAG
AAGCCCCACTGGGCCTCTATTTCTCAAGGGATGCTTACTGGGAGAGGCTGTATGTAGACCAGCCAGCTGG
CACACCTCTGCTCTATGTCCATGCCCTACGGGATGCCCCTGGAGAAGTGCCGAGCTTCCGCCTGGGCCAG
CATCTCTATGGCGTCTACCGTACACGGCTGCATGAGAATGACTGGATCCGCATCAATGAGACTACTGGCC
TTCTCTACCTCAATCAGAGCCTGGACCACAGTTCCTGGGAACAGCTCAGCATCCGCAATGGTGGTTTCCC
CCTGCTCACCATCTTCCTCCAGGTCTTTCTGGGGTCCACAGCCCAGAGAGAGGGAGAATGCCATTGGCCA
GGCTGTACCCGTGTGTACTTCTCCTTCATCAACGACACCTTCCCAAATTGTAGCTCCTTCAAAGCCCAGG
ATCTCTGCATCCCAGAGACAGCCGTGTCCTTCCGAGTCAGGGAGAACAGGCCTCCTGGCACCTTCTACCA
CTTCCACATGTTACCCGTGCAGTTCCTTTGTCCCAACATCAGTGTGAAGTACAGTCTCTTAGGAGGGGAT
AGTCTGCCCTTCCGTTGTGACCCAGACTGCCTGGAGGTGAGCACTCGCTGGGCCCTGGATCGAGAGCTCC
GGGAGAAGTATGTGCTGGAGGCTTTGTGCATAGTGGCAGGCCCTGGTGCCAACAAAGAGACGGTGACTCT
GTCCTTCCCAGTGACAGTGTATGATGAGGACGACTCGGCGCCCACCTTCTCTGGAGGTGTGGGCACTGCC
AGCGCGGTGGTGGAGTTTAAGCGGAAGGAGGGCACTGTGGTGGCCACCCTGCAGGTGTTCGATGCAGATG
TGGTGCCAGCGTCTGGGGAGCTGGTGAGACGGTACACAAACACACTCCTCTCAGGGGACTCCTGGGCCCA
GCAGACCTTCCGGGTGGAGCATTCGCCCATCGAGACCTTGGTCCAGGTCAACAACAACTCCGTTCGGGCA
ACCATGCACAATTACAAGCTGATTCTCAACAGGAGCCTGTCTATCTCAGAGAGCCGAGTCCTGCAGCTCG
CGGTCCTGGTCAACGACTCAGACTTCCAGGGGCCTGGGGCAGGTGGTATCCTCGTCCTCCATTTCAACGT
GTCTGTACTGCCCGTCACCCTGAACCTACCCAGGGCCTACTCCTTCCCAGTGAATAAGAGGGCCCGCCGC
TATGCCCAGATCGGGAAAGTCTGTGTGGAAAACTGCCAGGAGTTCAGCGGTGTCTCCATCCAGTACAAGC
TGCAGCCTTCCAGCATCAACTGCACTGCCCTAGGTGTGGTCACCTCACCCGAGGACACCTCGGGGACCCT
ATTTGTAAATGACACAGAGGCCCTGCGGCGACCTGAGTGCACCAAGCTTCAGTACACGGTGGTAGCCACT
GACCGGCAGACCCGCAGACAGACCCAGGCTTCGCTAGTGGTCACTGTGGAGGGGACATCCATTACTGAAG
AAGTAGGCTGCCCCAAGTCCTGTGCAGTAAACAAGAGGCGCCCCGAGTGTGAGGAATGTGGTGGCCTGGG
TTCTCCAACTGGCAGGTGCGAGTGGCGCCAGGGAGATGGTAAAGGGATCACCAGGAACTTCTCCACCTGC
TCCCCCAGTACCAGGACCTGCCCCGACGGCCACTGTGATGCTGTGGAGAGCCGGGATGCCAACATTTGCC
CCCAGGACTGTCTCCGTGCCGACATTGTTGGAGGACACGAGCGAGGGGAGCGCCAGGGCATTAAAGCAGG
CTACGGCATCTGCAACTGTTTCCCTGATGAGAAGAAATGCTTCTGCGAGCCAGAGGACAGCCAGGGCCCA
CTGTGTGATGCGCTGTGCCGCACGATCATCACAGCTGCCCTCTTCTCCCTTATCATCTCCATCCTGCTGT
CCATCTTCTGTGTCTGCCACCACCACAAGCATGGGCACAAGCCGCCCATTGCATCAGCGGAAATGACCTT
CTGCCGGCCGGCCCAGGGCTTCCCAATCAGTTATTCCTCCTCAGGCACCCGCCGGCCCTCACTGGATTCC
ACGGAGAACCAGGTTCCTGTGGACTCTTTCAAGATCCCGGAGGATCCAAAGTGGGAATTTCCTCGGAAGA
ACTTAGTTCTTGGGAAAACTCTGGGAGAAGGCGAGTTTGGAAAAGTTGTCAAGGCCACAGCCTTCCGTCT
GAAAGGCCGGGCAGGATACACCACAGTGGCTGTGAAAATGCTGAAAGAAAACGCCTCCCAGAGTGAGTTA
CGAGACCTGCTGTCTGAGTTCAACCTTCTGAAACAAGTCAACCATCCACATGTCATCAAGTTGTATGGGG
CCTGCAGCCAGGATGGGCCACTTCTTCTCATTGTGGAGTATGCCAAGTATGGCTCCCTGCGGGGATTCCT
CCGTGACAGCCGCAAGATTGGGCCTGCCTATGTGAGCGGTGGAGGCAGCCGCAACTCCAGCTCGCTGGAC
CACCCAGATGAAAGGGTACTGACCATGGGTGACCTCATCTCCTTCGCCTGGCAGATCTCGAGGGGTATGC
AGTACTTGGCAGAAATGAAGCTTGTACATCGGGACTTAGCTGCCAGGAACATCTTGGTGGCTGAGGGACG
GAAGATGAAGATTTCCGACTTTGGGCTGTCCCGAGATGTTTATGAGGAAGATTCCTATGTGAAGAAAAGC
AAGGGCCGGATTCCCGTCAAGTGGATGGCAATTGAGTCCCTTTTCGATCACATCTATACTACTCAAAGTG
ATGTGTGGTCCTTTGGAGTGCTGCTCTGGGAGATTGTGACCCTGGGAGGCAACCCCTACCCTGGAATTCC
TCCTGAACGACTCTTCAACCTTCTGAAGACAGGCCACAGGATGGAGAGGCCAGACAACTGCAGCGAGGAA
ATGTACCGTCTGATGCTGCAGTGCTGGAAGCAGGAGCCAGACAAGAGGCCAGTGTTTGCTGACATCAGCA
AGGATCTGGAGAAGATGATGGTCAAGAGCAGAGACTACTTGGACCTGGCTGCATCCACACCTTCGGACTC
ACTGCTGTATGACGATGGGCTCTCAGAAGAGGAGACACCCCTGGTGGACTGTAACAATGCTCCCCTCCCG
CGCTCCCTCCCTTCCACATGGATTGAAAACAAACTCTATGGCATGTCAGACCCGAACTGGCCTGGAGAGA
GTCCTGTACCACTCACGAGAGCCGATGGCACTAGCACTGGGTTCCCAAGATATGCAAATGATAGTGTATA
TGCTAACTGGATGGTTTCACCCTCAGCGGCAAAATTAATGGACACATTTGATAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR226724 representing NM_009050
Red=Cloning site Green=Tags(s)

MAKATSGAAGLGLKLILLLPLLGEAPLGLYFSRDAYWERLYVDQPAGTPLLYVHALRDAPGEVPSFRLGQ
HLYGVYRTRLHENDWIRINETTGLLYLNQSLDHSSWEQLSIRNGGFPLLTIFLQVFLGSTAQREGECHWP
GCTRVYFSFINDTFPNCSSFKAQDLCIPETAVSFRVRENRPPGTFYHFHMLPVQFLCPNISVKYSLLGGD
SLPFRCDPDCLEVSTRWALDRELREKYVLEALCIVAGPGANKETVTLSFPVTVYDEDDSAPTFSGGVGTA
SAVVEFKRKEGTVVATLQVFDADVVPASGELVRRYTNTLLSGDSWAQQTFRVEHSPIETLVQVNNNSVRA
TMHNYKLILNRSLSISESRVLQLAVLVNDSDFQGPGAGGILVLHFNVSVLPVTLNLPRAYSFPVNKRARR
YAQIGKVCVENCQEFSGVSIQYKLQPSSINCTALGVVTSPEDTSGTLFVNDTEALRRPECTKLQYTVVAT
DRQTRRQTQASLVVTVEGTSITEEVGCPKSCAVNKRRPECEECGGLGSPTGRCEWRQGDGKGITRNFSTC
SPSTRTCPDGHCDAVESRDANICPQDCLRADIVGGHERGERQGIKAGYGICNCFPDEKKCFCEPEDSQGP
LCDALCRTIITAALFSLIISILLSIFCVCHHHKHGHKPPIASAEMTFCRPAQGFPISYSSSGTRRPSLDS
TENQVPVDSFKIPEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFRLKGRAGYTTVAVKMLKENASQSEL
RDLLSEFNLLKQVNHPHVIKLYGACSQDGPLLLIVEYAKYGSLRGFLRDSRKIGPAYVSGGGSRNSSSLD
HPDERVLTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNILVAEGRKMKISDFGLSRDVYEEDSYVKKS
KGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIPPERLFNLLKTGHRMERPDNCSEE
MYRLMLQCWKQEPDKRPVFADISKDLEKMMVKSRDYLDLAASTPSDSLLYDDGLSEEETPLVDCNNAPLP
RSLPSTWIENKLYGMSDPNWPGESPVPLTRADGTSTGFPRYANDSVYANWMVSPSAAKLMDTFDS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_009050
ORF Size 3345 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_009050.2, NP_033076.2
RefSeq Size 6064 bp
RefSeq ORF 3348 bp
Locus ID 19713
Cytogenetics 6 55.86 cM
MW 124.3 kDa

Citations (1)

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68 Mouse Clones
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