Ctnnd1 (NM_007615) Mouse Tagged ORF Clone

CAT#: MR226707

Ctnnd1 (Myc-DDK-tagged) - Mouse catenin (cadherin associated protein), delta 1 (Ctnnd1), transcript variant 1

Product Images

Circular map for MR226707

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ctnnd1
• Synonyms: AA409437; AU019353; Catns; Ctnnd; mKIAA0384; P120
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226707 representing NM_007615
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACTCAGAGGTGGAGTCGACCGCCAGCATCTTGGCCTCTGTGAAAGAACAAGAGGCCCAGTTTG
  AGAAGCTGACCCGGGCGCTGGAAGAGGAGCGGCGCCATGTCTCGGCGCAACTGGAACGCGTCCGGGTCTC
  ACCACAAGATGCCAACTCACTCATGGCCAATGGCACCCTCACTCGTCGGCATCAGAACGGCCGGTTTGTG
  GGCGATGCTGACCTTGAGAGACAGAAATTTTCAGATCTGAAACTCAACGGACCCCAGGATCACAACCACC
  TTCTGTACAGCACTATCCCCAGGATGCAGGAGCCAGGACAGATTGTGGAAACCTACACCGAGGAGGACCC
  TGAAGGAGCCATGTCTGTTGTTTCTGTGGAGACCACAGATGATGGGACCACTAGACGCACGGAGACCACA
  GTCAAAAAAGTTGTGAAGACAATGACAACTCGGACAGTACAGCCTGTCCCCATGGGGCCAGACGGGCTGC
  CCGTGGACGCCTCAGCAGTCTCCAATAACTATATCCAGACCTTGGGCCGTGATTTTCGCAAGAATGGCAA
  TGGGGGCCCTGGTCCCTATGTGGGGCAAGCGGGCACTGCCACTCTTCCTAGGAACTTCCACTATCCTCCA
  GATGGGTATGGCCGACACTATGAAGATGGTTATCCAGGTGGCAGTGACAACTACGGCAGTCTGTCCCGGG
  TGACCCGAATTGAGGAGCGGTATAGGCCCAGCATGGAAGGCTACCGGGCACCCAGTAGACAAGATGTCTA
  TGGGCCCCAGCCCCAGGTTCGAGTAGGTGGAAGCAGTGTGGACCTGCATCGTTTTCATCCTGAGCCTTAC
  GGGCTAGAGGATGACCAGCGCAGCATGGGCTATGATGACCTCGATTACGGCATGATGTCTGATTATGGTA
  CTGCCCGTCGGACAGGAACACCCTCTGACCCTCGACGACGCCTCAGGAGCTATGAAGACATGATTGGTGA
  AGAGGTGCCGCCTGATCAGTACTATTGGGCTCCTTTAGCTCAGCATGAGCGGGGCAGTTTAGCAAGCTTG
  GATAGTTTGCGAAAGGGAATGCCCCCACCTTCAAACTGGAGACAGCCTGAGCTGCCAGAGGTGATTGCCA
  TGTTAGGCTTCCGCTTGGATGCTGTTAAGTCCAATGCAGCTGCATACCTGCAGCACTTATGCTACCGCAA
  TGACAAGGTGAAGACTGACGTGCGGAAGCTCAAGGGTATCCCGATATTGGTAGGATTGTTAGACCATCCC
  AAAAAGGAAGTGCACCTCGGAGCCTGTGGAGCTCTCAAGAATATCTCTTTTGGACGTGACCAAGATAACA
  AGATCGCCATAAAAAACTGTGATGGTGTTCCTGCTCTGGTCCGATTGCTCCGAAAGGCTCGTGATATGGA
  CCTGACTGAAGTGATTACTGGAACCCTGTGGAATCTCTCATCCCATGATTCAATCAAAATGGAGATTGTG
  GACCATGCGCTACATGCCTTGACAGATGAAGTTATCATTCCACATTCTGGTTGGGAGAGAGAACCTAATG
  AAGACTGTAAGCCACGGCATATTGAATGGGAGTCAGTGCTTACCAACACTGCTGGCTGCCTTCGGAACGT
  AAGCTCAGAGAGGAGTGAAGCCCGGAGGAAACTTCGGGAATGTGACGGCTTAGTTGATGCCCTCATTTTC
  ATCGTTCAGGCAGAAATTGGGCAGAAGGATTCAGACAGTAAGCTTGTGGAGAATTGTGTTTGCCTCCTCC
  GGAACTTATCATACCAAGTTCATCGTGAAATCCCACAGGCAGAGCGTTACCAGGAGGCCCTTCCTACTGT
  GGCTAACAGTACTGGGCCACACGCTGCCAGTTGCTTTGGAGCCAAGAAGGGCAAAGATGAGTGGTTCTCC
  AGAGGGAAAAAGCCTACAGAGGATCCAGCAAATGATACAGTGGATTTCCCTAAAAGAACTAGTCCTGCTC
  GAGGCTATGAACTCTTATTTCAGCCAGAAGTGGTGCGAATATACATTTCACTCCTTAAGGAGAGCAAGAC
  GCCTGCCATCTTAGAAGCCTCGGCCGGAGCTATCCAGAACTTGTGTGCTGGGCGTTGGACATATGGCAGA
  TACATCCGCTCTGCTCTTCGTCAGGAGAAGGCTCTCTCTGCCATAGCTGAGCTCCTTACGAGTGAGCATG
  AGCGAGTAGTAAAAGCTGCTTCTGGGGCACTGAGAAATCTGGCTGTGGATGCTCGGAACAAAGAGTTAAT
  TGGCAAGCATGCTATTCCTAACTTGGTAAAGAATCTGCCAGGAGGTCAACAGAACTCCTCCTGGAATTTT
  TCTGAAGATACTGTGGTCTCCATATTGAACACCATCAACGAAGTTATCGCTGAGAACTTAGAAGCTGCCA
  AAAAGCTTCGAGAGACGCAGGGTATTGAGAAGCTGGTGTTGATCAACAAATCAGGGAATCGTTCAGAAAA
  AGAAGTCCGGGCAGCAGCTCTTGTCCTGCAGACAATCTGGGGCTATAAGGAGCTTCGGAAGCCACTGGAA
  AAAGAAGGATGGAAGAAATCAGACTTCCAGGTGAATCTAAACAATGCATCTAGAAGCCAGAGCAGCCATT
  CATATGATGATAGCACTCTCCCCCTCATTGACCGGAATCAAAAATCAGATAAGAAACCTGACCGTGAAGA
  AATTCCAATGAGCAATATGGGGTCAAACACAAAATCATTAGATAACAACTATTCCACACTGAATGAGAGA
  GGAGACCACAACAGAACACTGGACCGATCTGGGGATCTGGGTGATATGGAACCATTGAAGGGAGCACCCT
  TGATGCAGAAGATT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226707 representing NM_007615
  Red=Cloning site Green=Tags(s)
  
  MDDSEVESTASILASVKEQEAQFEKLTRALEEERRHVSAQLERVRVSPQDANSLMANGTLTRRHQNGRFV
  GDADLERQKFSDLKLNGPQDHNHLLYSTIPRMQEPGQIVETYTEEDPEGAMSVVSVETTDDGTTRRTETT
  VKKVVKTMTTRTVQPVPMGPDGLPVDASAVSNNYIQTLGRDFRKNGNGGPGPYVGQAGTATLPRNFHYPP
  DGYGRHYEDGYPGGSDNYGSLSRVTRIEERYRPSMEGYRAPSRQDVYGPQPQVRVGGSSVDLHRFHPEPY
  GLEDDQRSMGYDDLDYGMMSDYGTARRTGTPSDPRRRLRSYEDMIGEEVPPDQYYWAPLAQHERGSLASL
  DSLRKGMPPPSNWRQPELPEVIAMLGFRLDAVKSNAAAYLQHLCYRNDKVKTDVRKLKGIPILVGLLDHP
  KKEVHLGACGALKNISFGRDQDNKIAIKNCDGVPALVRLLRKARDMDLTEVITGTLWNLSSHDSIKMEIV
  DHALHALTDEVIIPHSGWEREPNEDCKPRHIEWESVLTNTAGCLRNVSSERSEARRKLRECDGLVDALIF
  IVQAEIGQKDSDSKLVENCVCLLRNLSYQVHREIPQAERYQEALPTVANSTGPHAASCFGAKKGKDEWFS
  RGKKPTEDPANDTVDFPKRTSPARGYELLFQPEVVRIYISLLKESKTPAILEASAGAIQNLCAGRWTYGR
  YIRSALRQEKALSAIAELLTSEHERVVKAASGALRNLAVDARNKELIGKHAIPNLVKNLPGGQQNSSWNF
  SEDTVVSILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQTIWGYKELRKPLE
  KEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIPMSNMGSNTKSLDNNYSTLNER
  GDHNRTLDRSGDLGDMEPLKGAPLMQKI
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007615
• ORF Size: 2814 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007615.2
• RefSeq Size: 5475 bp
• RefSeq ORF: 2817 bp
• Locus ID: 12388
• UniProt ID: P30999
• Cytogenetics: 2 49.45 cM
• MW: 105.4 kDa
• Gene Summary: Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). Associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.[UniProtKB/Swiss-Prot Function]