Col1a1 (NM_007742) Mouse Tagged ORF Clone

CAT#: MR226667

Col1a1 (Myc-DDK-tagged) - Mouse collagen, type I, alpha 1 (Col1a1)

Product Images

Circular map for MR226667

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Col1a1
• Synonyms: Co; Col; Col1; Col1a-1; Cola-1; Cola1; Mov-; Mov-13; Mov13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226667 representing NM_007742
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAGCTTTGTGGACCTCCGGCTCCTGCTCCTCTTAGGGGCCACTGCCCTCCTGACGCATGGCCAAG
  AAGACATCCCTGAAGTCAGCTGCATACACAATGGCCTAAGGGTCCCCAATGGTGAGACGTGGAAACCCGA
  GGTATGCTTGATCTGTATCTGCCACAATGGCACGGCTGTGTGCGATGACGTGCAATGCAATGAAGAACTG
  GACTGTCCCAACCCCCAAAGACGGGAGGGCGAGTGCTGTGCTTTCTGCCCGGAAGAATACGTATCACCAA
  ACTCAGAAGATGTAGGAGTCGAGGGACCCAAGGGAGACCCTGGCCCCCAAGGCCCAAGGGGACCCGTTGG
  CCCCCCTGGACGAGATGGCATCCCTGGACAGCCTGGACTTCCTGGTCCTCCTGGTCCCCCTGGGCCCCCC
  GGACCCCCTGGCCTTGGAGGAAACTTTGCTTCCCAGATGTCCTATGGCTATGATGAAAAATCAGCTGGAG
  TTTCCGTGCCTGGCCCCATGGGTCCTTCTGGTCCTCGTGGTCTCCCTGGCCCCCCTGGTGCACCTGGTCC
  ACAAGGTTTCCAAGGCCCCCCTGGTGAACCTGGCGAGCCTGGCGGTTCAGGTCCAATGGGTCCCCGAGGT
  CCCCCTGGCCCTCCTGGCAAGAATGGAGATGATGGGGAAGCTGGCAAGCCCGGCCGTCCTGGTGAGCGTG
  GACCTCCTGGACCTCAGGGTGCTCGTGGATTGCCTGGAACAGCTGGCCTCCCTGGAATGAAGGGACACCG
  AGGCTTCAGTGGTTTGGATGGTGCCAAAGGAGATGCTGGTCCTGCTGGTCCTAAGGGAGAGCCCGGCAGT
  CCTGGTGAAAACGGAGCTCCTGGCCAGATGGGTCCCCGAGGTCTGCCCGGTGAGAGAGGTCGCCCTGGAC
  CTCCTGGCACTGCTGGTGCTCGCGGTAACGATGGTGCTGTTGGTGCTGCTGGACCCCCTGGTCCCACCGG
  CCCCACTGGCCCTCCTGGCTTCCCTGGTGCAGTTGGTGCTAAGGGTGAAGCTGGTCCCCAAGGAGCTAGA
  GGCTCTGAAGGTCCCCAGGGTGTGCGTGGTGAGCCCGGACCCCCTGGCCCTGCTGGTGCTGCCGGCCCTG
  CTGGAAACCCTGGTGCTGATGGACAACCTGGCGCTAAAGGTGCCAATGGTGCTCCTGGTATTGCTGGTGC
  TCCTGGCTTCCCTGGTGCCCGAGGCCCCTCTGGACCCCAGGGCCCCAGCGGCCCTCCAGGTCCCAAGGGT
  AACAGTGGTGAACCTGGTGCTCCTGGCAACAAAGGAGACACTGGTGCCAAAGGAGAACCCGGTGCTACTG
  GAGTTCAAGGTCCCCCAGGCCCTGCCGGAGAAGAAGGAAAACGAGGAGCCCGTGGTGAGCCTGGACCTTC
  CGGACTGCCTGGACCTCCTGGCGAGCGTGGTGGACCTGGTAGCCGTGGTTTCCCTGGTGCTGATGGTGTT
  GCTGGCCCCAAGGGTCCTTCCGGTGAACGTGGTGCTCCCGGACCTGCTGGTCCCAAAGGTTCTCCTGGTG
  AAGCTGGTCGCCCCGGTGAAGCTGGTCTCCCTGGTGCCAAGGGTCTCACTGGCAGTCCTGGCAGCCCTGG
  TCCTGATGGCAAAACCGGCCCCCCTGGTCCCGCTGGTCAAGATGGTCGCCCTGGACCCGCAGGTCCTCCT
  GGAGCCCGTGGCCAGGCTGGTGTGATGGGATTCCCTGGACCTAAGGGTACCGCTGGAGAACCTGGAAAGG
  CTGGAGAGCGAGGCCTTCCCGGACCCCCTGGCGCTGTTGGTCCTGCTGGCAAAGATGGAGAAGCTGGAGC
  TCAGGGAGCCCCTGGCCCTGCTGGTCCTGCTGGTGAGAGAGGTGAACAAGGTCCCGCTGGCTCCCCTGGA
  TTCCAGGGTCTTCCTGGTCCTGCCGGTCCTCCTGGTGAAGCAGGCAAGCCTGGTGAACAGGGTGTTCCTG
  GAGACCTTGGTGCCCCTGGACCCTCTGGCGCAAGAGGCGAGAGAGGTTTCCCTGGTGAACGTGGTGTACA
  AGGTCCCCCAGGTCCTGCTGGTCCCCGAGGAAACAATGGTGCCCCCGGCAACGATGGTGCCAAGGGTGAT
  ACTGGTGCCCCCGGAGCTCCCGGTAGCCAGGGTGCCCCCGGTCTTCAGGGAATGCCTGGTGAACGTGGTG
  CAGCTGGTCTTCCAGGTCCTAAGGGTGACAGAGGTGATGCTGGTCCCAAAGGTGCTGATGGTTCTCCTGG
  TAAAGATGGTGCCCGTGGTCTGACTGGTCCCATTGGTCCTCCTGGCCCTGCTGGTGCCCCTGGTGACAAG
  GGTGAAGCTGGTCCCAGTGGTCCTCCCGGTCCCACCGGAGCCCGTGGTGCTCCCGGAGACCGTGGTGAGG
  CTGGTCCCCCTGGTCCTGCTGGCTTTGCCGGCCCCCCTGGTGCTGATGGCCAACCTGGTGCGAAAGGTGA
  ACCTGGTGATACTGGTGTTAAAGGTGATGCTGGTCCTCCTGGCCCTGCTGGTCCTGCTGGACCCCCCGGC
  CCCATTGGTAACGTTGGTGCTCCTGGACCCAAAGGTCCTCGTGGTGCTGCTGGTCCCCCTGGTGCTACTG
  GCTTCCCTGGTGCTGCTGGCCGTGTCGGTCCCCCTGGTCCCTCTGGAAATGCTGGACCCCCTGGCCCTCC
  CGGTCCCGTTGGCAAAGAAGGGGGCAAAGGTCCCCGTGGTGAGACTGGCCCTGCTGGACGTCCTGGTGAA
  GTTGGTCCCCCAGGTCCCCCCGGTCCTGCTGGTGAGAAAGGATCTCCTGGTGCTGATGGACCTGCTGGCT
  CTCCTGGTACCCCTGGACCTCAGGGTATTGCTGGACAACGTGGTGTGGTCGGTCTTCCCGGTCAGAGAGG
  AGAAAGAGGCTTCCCTGGTCTTCCTGGCCCCTCTGGTGAACCTGGCAAACAAGGTCCTTCTGGATCAAGT
  GGTGAACGCGGTCCCCCTGGCCCCATGGGGCCCCCTGGATTGGCTGGTCCCCCTGGTGAATCTGGACGTG
  AGGGATCCCCTGGTGCTGAAGGCTCCCCTGGAAGGGATGGTGCTCCCGGGGCCAAGGGTGACCGTGGTGA
  GACTGGCCCCGCTGGCCCCCCTGGTGCCCCTGGTGCTCCCGGTGCTCCCGGCCCTGTTGGTCCCGCTGGC
  AAGAATGGCGATCGTGGTGAGACTGGTCCTGCTGGTCCTGCTGGTCCCATTGGCCCTGCTGGTGCCCGTG
  GCCCTGCTGGACCCCAAGGCCCCCGTGGTGACAAGGGTGAGACAGGCGAACAAGGTGACAGAGGCATAAA
  GGGTCATCGTGGCTTCTCTGGTCTCCAGGGTCCTCCTGGTTCTCCTGGTTCTCCTGGTGAACAAGGCCCC
  TCTGGAGCTTCAGGTCCTGCAGGCCCCCGGGGTCCCCCTGGCTCTGCTGGTTCTCCTGGCAAAGACGGAC
  TCAACGGTCTCCCTGGCCCCATTGGTCCCCCTGGTCCTCGAGGTCGCACTGGTGACAGCGGCCCTGCTGG
  TCCCCCCGGCCCTCCTGGACCCCCTGGCCCTCCTGGACCTCCCAGTGGCGGTTATGACTTCAGCTTCCTG
  CCTCAGCCACCTCAAGAGAAGTCTCAAGATGGTGGCCGCTACTACCGGGCCGATGATGCTAACGTGGTTC
  GTGACCGTGACCTTGAGGTGGACACCACCCTCAAGAGCCTGAGTCAGCAGATTGAGAACATCCGCAGCCC
  CGAAGGCAGCCGCAAGAACCCTGCCCGCACATGCCGCGACCTCAAGATGTGCCACTCTGACTGGAAGAGC
  GGAGAGTACTGGATCGACCCTAACCAAGGCTGCAACCTGGACGCCATCAAGGTCTACTGCAACATGGAGA
  CAGGTCAGACCTGTGTGTTCCCTACTCAGCCGTCTGTGCCTCAGAAGAACTGGTACATCAGCCCGAACCC
  CAAGGAAAAGAAGCACGTCTGGTTTGGAGAGAGCATGACCGATGGATTCCCGTTCGAGTACGGAAGCGAG
  GGCTCCGACCCCGCCGATGTCGCTATCCAGCTGACCTTCCTGCGCCTAATGTCCACCGAGGCCTCCCAGA
  ACATCACCTATCACTGCAAGAACAGCGTAGCCTACATGGACCAGCAGACTGGCAACCTCAAGAAGGCCCT
  GCTCCTCCAGGGATCCAACGAGATCGAGCTCAGAGGCGAAGGCAACAGTCGCTTCACCTACAGCACCCTT
  GTGGACGGCTGCACGAGTCACACCGGAACTTGGGGCAAGACAGTCATCGAATACAAAACCACCAAGACCT
  CCCGCCTGCCCATCATCGATGTGGCTCCCTTGGACATTGGTGCCCCAGACCAGGAATTCGGACTAGACAT
  TGGCCCTGCCTGCTTCGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226667 representing NM_007742
  Red=Cloning site Green=Tags(s)
  
  MFSFVDLRLLLLLGATALLTHGQEDIPEVSCIHNGLRVPNGETWKPEVCLICICHNGTAVCDDVQCNEEL
  DCPNPQRREGECCAFCPEEYVSPNSEDVGVEGPKGDPGPQGPRGPVGPPGRDGIPGQPGLPGPPGPPGPP
  GPPGLGGNFASQMSYGYDEKSAGVSVPGPMGPSGPRGLPGPPGAPGPQGFQGPPGEPGEPGGSGPMGPRG
  PPGPPGKNGDDGEAGKPGRPGERGPPGPQGARGLPGTAGLPGMKGHRGFSGLDGAKGDAGPAGPKGEPGS
  PGENGAPGQMGPRGLPGERGRPGPPGTAGARGNDGAVGAAGPPGPTGPTGPPGFPGAVGAKGEAGPQGAR
  GSEGPQGVRGEPGPPGPAGAAGPAGNPGADGQPGAKGANGAPGIAGAPGFPGARGPSGPQGPSGPPGPKG
  NSGEPGAPGNKGDTGAKGEPGATGVQGPPGPAGEEGKRGARGEPGPSGLPGPPGERGGPGSRGFPGADGV
  AGPKGPSGERGAPGPAGPKGSPGEAGRPGEAGLPGAKGLTGSPGSPGPDGKTGPPGPAGQDGRPGPAGPP
  GARGQAGVMGFPGPKGTAGEPGKAGERGLPGPPGAVGPAGKDGEAGAQGAPGPAGPAGERGEQGPAGSPG
  FQGLPGPAGPPGEAGKPGEQGVPGDLGAPGPSGARGERGFPGERGVQGPPGPAGPRGNNGAPGNDGAKGD
  TGAPGAPGSQGAPGLQGMPGERGAAGLPGPKGDRGDAGPKGADGSPGKDGARGLTGPIGPPGPAGAPGDK
  GEAGPSGPPGPTGARGAPGDRGEAGPPGPAGFAGPPGADGQPGAKGEPGDTGVKGDAGPPGPAGPAGPPG
  PIGNVGAPGPKGPRGAAGPPGATGFPGAAGRVGPPGPSGNAGPPGPPGPVGKEGGKGPRGETGPAGRPGE
  VGPPGPPGPAGEKGSPGADGPAGSPGTPGPQGIAGQRGVVGLPGQRGERGFPGLPGPSGEPGKQGPSGSS
  GERGPPGPMGPPGLAGPPGESGREGSPGAEGSPGRDGAPGAKGDRGETGPAGPPGAPGAPGAPGPVGPAG
  KNGDRGETGPAGPAGPIGPAGARGPAGPQGPRGDKGETGEQGDRGIKGHRGFSGLQGPPGSPGSPGEQGP
  SGASGPAGPRGPPGSAGSPGKDGLNGLPGPIGPPGPRGRTGDSGPAGPPGPPGPPGPPGPPSGGYDFSFL
  PQPPQEKSQDGGRYYRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMCHSDWKS
  GEYWIDPNQGCNLDAIKVYCNMETGQTCVFPTQPSVPQKNWYISPNPKEKKHVWFGESMTDGFPFEYGSE
  GSDPADVAIQLTFLRLMSTEASQNITYHCKNSVAYMDQQTGNLKKALLLQGSNEIELRGEGNSRFTYSTL
  VDGCTSHTGTWGKTVIEYKTTKTSRLPIIDVAPLDIGAPDQEFGLDIGPACFV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007742
• ORF Size: 4359 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007742.4, NP_031768.2
• RefSeq Size: 4709 bp
• RefSeq ORF: 4362 bp
• Locus ID: 12842
• UniProt ID: P11087
• Cytogenetics: 11 D
• MW: 138.5 kDa
• Gene Summary: This gene encodes the alpha-1 subunit of the fibril-forming type I collagen, the most abundant protein of bone, skin and tendon extracellular matrices. The encoded protein, in association with alpha-2 subunit, forms heterotrimeric type I procollagen that undergoes proteolytic processing during fibril formation. Mice lacking the encoded protein die in utero caused by the rupture of a major blood vessel. Transgenic mice expressing significantly lower levels of this gene exhibit morphological and functional defects in mineralized and non-mineralized connective tissue and, progressive loss of hearing. [provided by RefSeq, Nov 2015]