Fan1 (NM_177893) Mouse Tagged Lenti ORF Clone
CAT#: MR226479L4
- LentiORF®
Lenti ORF clone of Fan1 (mGFP-tagged) - Mouse FANCD2/FANCI-associated nuclease 1 (Fan1)
"NM_177893" in other vectors (4)
Interest in protein/lysate? Submit request here!
USD 365.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Symbol | Fan1 |
Synonyms | 6030441H18Rik; mFAN1; Mtmr15 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR226479).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_177893 |
ORF Size | 3060 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_177893.3 |
RefSeq Size | 3475 bp |
RefSeq ORF | 3063 bp |
Locus ID | 330554 |
UniProt ID | Q69ZT1 |
Cytogenetics | 7 C |
Gene Summary | Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates (By similarity). Not involved in DNA double-strand breaks resection. Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions (PubMed:24981866). Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL. Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap (By similarity). Also has endonuclease activity toward 5'-flaps (PubMed:24981866).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223261 | Fan1 (untagged) - Mouse FANCD2/FANCI-associated nuclease 1 (Fan1), (10ug) |
USD 978.00 |
|
MG226479 | Fan1 (tGFP-tagged) - Mouse FANCD2/FANCI-associated nuclease 1 (Fan1), (10ug) |
USD 1,088.00 |
|
MR226479 | Fan1 (Myc-DDK-tagged) - Mouse FANCD2/FANCI-associated nuclease 1 (Fan1) |
USD 888.00 |
|
MR226479L3 | Lenti ORF clone of Fan1 (Myc-DDK-tagged) - Mouse FANCD2/FANCI-associated nuclease 1 (Fan1) |
USD 1,188.00 |
{0} Product Review(s)
Be the first one to submit a review