Carns1 (NM_134148) Mouse Tagged ORF Clone

CAT#: MR226464

Carns1 (Myc-DDK-tagged) - Mouse carnosine synthase 1 (Carns1)

Product Images

Circular map for MR226464

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Carns1
• Synonyms: AI790298; Atpgd1; mKIAA1394
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226464 representing NM_134148
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTGCCTGGATCCACTGGGTACGGAGTGGGACTCCAAGGACCTGGATGGAAAGGAAGAACCATGGA
  AAAGCGGTGCTGGCCTGCCACCCACAGGCTGCTTCCCTGGGCCCTGGCGCCAGGATATCAGCCTGGATTG
  CAAGGGCTCCCCGGAAGAGACTGAGGCTCGGGCCTGGACTGTGTACTACTACGGTCTCCTTCAGAGTTGT
  CTGCAGCAAGCTGGCCTTCCTGAGACCCAGGATCGCAGCCAGGCACCTCGTACAGGCTGCCCTGGGGCAG
  AGGTGACCTTGTGCATTCTGGGTTCTCCCAGCACCTTCCTATCTCTGTTGCTGGAGGGTGGGGTCCAGAG
  CCCTGGAAACATGCTCCTTTGCCTGTCCCCTGCTTGGCTGATGAAGGTGGCAACTCCAGGCCAAGAGGGT
  GAGGCAGTCCTGCTGGTCTCTAAGGCTGTGAGCTTCTATCCAGGGGGCCTGACATTCCTGGATGACTTTG
  TTCCCCCTCGGCATGCTACTTACTTCCTGGCAGGCCTGGGGCCAGAATCTGGCCGTGGCAAGGAGGCAGC
  AGAGCTGGCTCGGAATCTCACTTGCCCCACAGGAACCTCATCGGAGCTGTCCCAGCTGCTGGAGAACCGG
  CTGCTGATGAGGTGGTTGCTGTCCCAGCAGAGTGGTGTGGCCGTGCCAGCTACCCTGGCTTTCACTTATA
  GGCCTCCGGGGCTGCTTCGGGGAGGGGATGCCAGCCCTGGACTCCGGCTGGTGGAACTCAGTGGGAAGGA
  GGGCCAGGAAACACTGGTGAAAGAGGAAGTAGAGGCCTTTGTGCACTCCGAGGCCTTGGGTGATGCTTCA
  CAGGTGGCTGTGAGGCTCAGTGGCTGTCGCTGGCGTGGACAGCAGGCATTGCCTCTGCACCTGCGGGTAG
  AGCCATCCACAGTGGTGAACACGGTGCTGGGATTGCTGGAGAAGCTGGAGGAGGAGGAGAGTGTCCTTGT
  GGAGGCCATGTGCCCACCTGTCCGGCTGCCCTTACCAGGCGGTCCTGCACCTGGCCCTGAGCTGGCTGTG
  CGTATCTGTGCAGTGGTGTGTCGGATTCAGGGTGATAGGCCCCTACTGAGTAAGGTGGTGTGTGGCGTGG
  GCCGTGGGGATCGACCCGTGCGGCACCACTATACCCTGCCAAGGACACTGAGGGTGGCGCTGGCTCAGTG
  TGGCCTGGAAGAGGAGGCGCAGGTGGCGCTTCTGGAGCAGGGCATCAAAGAAGCAGCCGAAGGCGCGTTG
  GCCGCTGTGCTGGCCCTGGAGGCTGGACTGAGTGTGGAGCAGCGTGGTGGACGCCAAGTGCACACCGACT
  TCCTCGGCGTGGACTTAGTTCTCACGGTGATCGGACGCACGCTGACGCCCGTGGTCCTTAAGCTGAACAG
  TGGCCTGTGCCTAGAGGCCTGCGGCGCGCTCGAGGGGCTGTGGGCCGTGCCACGCCTAAGACGCTCAGCT
  GAGGAGGCAGCGGCAGCGCCGCTGGTGGAGACTATGCTGCGGCGCTCAGGGCGCCACCTCATGGACGGCA
  AGCAGCTGCTGGTGATCGGCGCCGGCGGCGTCAGCAAGAAGTTCGTGTGGGAGGCGGCGCGGGACTACGG
  ACTGACGCTGCACCTGGTGGAATCAGACCCCAACCACTTTGCATCCCAACTGGTACAGACCTTTATCCAC
  TTTGATGTGACTGAGCACCGGAGGGATGAGGAGAATGCGCTGCTGCTGGCTGAGCTGGTACGGGCCCGGA
  ACCTGAAGCTAGATGGCTGCTTCTCCTTCTGGGATGACTGCTTGGTGCTTACTGCTCTCCTCTGCCGTGA
  GCTGGGTCTGCCCTGCAGCCCCCCAGCTGCCATGTGCTTGGCAAAGCAGAAGAGCCGCACACAGCTACAT
  CTGTTGCGGTGCCAGGGCCCACCCTGGCCTTCGACCTCTCTCCATGCTGTGGCCTGTTGCCCATTGGAGA
  ATGAGGCTGATGTGGAGAGAGCCATTTACCAGGTACCCCTGCCAGGTGTGATGAAACTGGAATTTGGGTC
  AGGAGCAGTAGGCGTGCAGCTGGTGAAGGATGGGCCCCAGTGCCGTGAGCACTTTTCCCGGATCCTCCAT
  GACCTGCAGGGTGAGGCTGACCACCCTGGCATCGGGCTGGGCTGGGGCAATGCTATGCTGCTGATGGAGT
  TTGTTGAGGGCACAGAGCATGACGTGGACCTGGTGGTGTTCGGTGGACGGTTGCTGGCTGCTTTCGTCTC
  CGATAATGGCCCCACAAGGCTGCCTGGCTTCACAGAGACTGCAGCCTGCATGCCCACTGGGCTAGCACCA
  GAGCAGGAGGCCCAGGTGGTGCAGGCAGCCTTTCGCTGCTGCCTGGGCTGTGGGCTGCTGGATGGGGTTT
  TCAATGTGGAGCTCAAGATGACTGGGGCTGGGCCAAGGCTCATCGAGATTAACCCTCGAATGGGAGGCTT
  CTACCTTCGAGACTGGATCCTAGAGCTCTATGGTGTGGATTTGCTTCTGGCTTCTACAATGGTGGCCTGT
  GGCCTGCAGCCTGCCTTGCCTGCTCACCCTCGTGCCCGTGGCTACCTAGTGGGCATCATGTGCCTGGTGT
  CCCAGCACCTACAGCTGCTGAGTTCCCCGAGCAGTAGAGAGACTCTGCAAACCCTCCATGACCAGGGGCA
  ATTACGACTCAACCTGCTGGAGGAGGCCCTGATACCTGGCCAGTATGAGGAGCCCTACTGCAATGTGGCC
  TGTGCTGGGCCCAGCCCTGCTGAGGCCTGTCACCGCCTGCTAGGCATCTGTCAGGGCTTGGGCATTGACA
  GGCCCAACTACCCTGTTGCCCATTTCCTGTCTCATTTCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226464 representing NM_134148
  Red=Cloning site Green=Tags(s)
  
  MLCLDPLGTEWDSKDLDGKEEPWKSGAGLPPTGCFPGPWRQDISLDCKGSPEETEARAWTVYYYGLLQSC
  LQQAGLPETQDRSQAPRTGCPGAEVTLCILGSPSTFLSLLLEGGVQSPGNMLLCLSPAWLMKVATPGQEG
  EAVLLVSKAVSFYPGGLTFLDDFVPPRHATYFLAGLGPESGRGKEAAELARNLTCPTGTSSELSQLLENR
  LLMRWLLSQQSGVAVPATLAFTYRPPGLLRGGDASPGLRLVELSGKEGQETLVKEEVEAFVHSEALGDAS
  QVAVRLSGCRWRGQQALPLHLRVEPSTVVNTVLGLLEKLEEEESVLVEAMCPPVRLPLPGGPAPGPELAV
  RICAVVCRIQGDRPLLSKVVCGVGRGDRPVRHHYTLPRTLRVALAQCGLEEEAQVALLEQGIKEAAEGAL
  AAVLALEAGLSVEQRGGRQVHTDFLGVDLVLTVIGRTLTPVVLKLNSGLCLEACGALEGLWAVPRLRRSA
  EEAAAAPLVETMLRRSGRHLMDGKQLLVIGAGGVSKKFVWEAARDYGLTLHLVESDPNHFASQLVQTFIH
  FDVTEHRRDEENALLLAELVRARNLKLDGCFSFWDDCLVLTALLCRELGLPCSPPAAMCLAKQKSRTQLH
  LLRCQGPPWPSTSLHAVACCPLENEADVERAIYQVPLPGVMKLEFGSGAVGVQLVKDGPQCREHFSRILH
  DLQGEADHPGIGLGWGNAMLLMEFVEGTEHDVDLVVFGGRLLAAFVSDNGPTRLPGFTETAACMPTGLAP
  EQEAQVVQAAFRCCLGCGLLDGVFNVELKMTGAGPRLIEINPRMGGFYLRDWILELYGVDLLLASTMVAC
  GLQPALPAHPRARGYLVGIMCLVSQHLQLLSSPSSRETLQTLHDQGQLRLNLLEEALIPGQYEEPYCNVA
  CAGPSPAEACHRLLGICQGLGIDRPNYPVAHFLSHFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134148
• ORF Size: 2841 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134148.2, NP_598909.2
• RefSeq Size: 3968 bp
• RefSeq ORF: 2844 bp
• Locus ID: 107239
• Cytogenetics: 19 A
• MW: 102.6 kDa
• Gene Summary: Catalyzes the synthesis of carnosine and homocarnosine. Carnosine is synthesized more efficiently than homocarnosine.[UniProtKB/Swiss-Prot Function]