Pnpla2 (NM_001163689) Mouse Tagged ORF Clone

CAT#: MR226260

Pnpla2 (Myc-DDK-tagged) - Mouse patatin-like phospholipase domain containing 2 (Pnpla2), transcript variant 1

Product Images

Circular map for MR226260

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pnpla2
• Synonyms: 0610039C21Rik; 1110001C14Rik; Atgl; TTS-2.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226260 representing NM_001163689
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCCGAGGGAGACCAAGTGGAACATCTCATTCGCTGGCTGCGGCTTCCTCGGGGTCTACCACATTG
  GCGTGGCCTCCTGCCTCCGTGAGCACGCGCCCTTCCTGGTGGCCAACGCCACTCACATCTACGGAGCCTC
  GGCAGGGGCGCTCACCGCCACAGCGCTGGTCACTGGGGCCTGCCTGGGTGAAGCAGGTGCCAACATTATT
  GAGGTGTCCAAGGAGGCCCGGAAGCGGTTCCTGGGTCCTCTGCATCCCTCCTTCAACCTGGTGAAGACCA
  TCCGTGGCTGTCTACTAAAGACCCTGCCTGCTGATTGCCATGAGCGCGCCAATGGACGCCTGGGCATCTC
  CCTGACTCGTGTTTCAGACGGAGAGAACGTCATCATATCCCACTTTAGCTCCAAGGATGAGCTCATCCAG
  GCCAATGTCTGCAGCACATTTATCCCGGTGTACTGTGGCCTCATTCCTCCTACCCTCCAAGGGGTGCGCT
  ATGTGGATGGCGGCATTTCAGACAACTTGCCACTTTATGAGCTGAAGAATACCATCACAGTGTCCCCATT
  CTCAGGCGAGAGTGACATCTGCCCTCAGGACAGCTCCACCAACATCCACGAGCTTCGCGTCACCAACACC
  AGCATCCAGTTCAACCTTCGCAATCTCTACCGCCTCTCGAAGGCTCTCTTCCCGCCAGAGCCCATGGTCC
  TCCGAGAGATGTGCAAACAGGGCTACAGAGATGGACTTCGATTCCTTAGGAGGAATGGCCTACTGAACCA
  ACCCAACCCTTTGCTGGCACTGCCCCCAGTTGTCCCCCAGGAAGAGGATGCAGAGGAAGCTGCTGTGGTG
  GAGGAGAGGGCTGGAGAGGAGGATCAATTGCAGCCTTATAGAAAAGATCGAATTCTAGAGCACCTGCCTG
  CCAGACTCAATGAGGCCCTGCTGGAGGCCTGTGTGGAACCAAAGGACCTGATGACCACCCTTTCCAACAT
  GCTACCAGTGCGCCTGGCAACGGCCATGATGGTGCCCTATACTCTGCCGCTGGAGAGTGCAGTGTCCTTC
  ACCATCCGCTTGTTGGAGTGGCTGCCTGATGTCCCTGAAGATATCCGGTGGATGAAAGAGCAGACGGGTA
  GCATCTGCCAGTATCTGGTGATGAGGGCCAAGAGGAAATTGGGTGGCCATCTGCCTTCCAGACTGTCTGA
  GCAGGTGGAACTGCGACGTGCCCAGTCTCTGCCCTCTGTGCCACTGTCTTGCGCCACCTACAGTGAGGCC
  CTACCCAACTGGGTACGAAACAACCTCTCACTGGGGGACGCGCTGGCCAAGTGGGAAGAATGCCAGCGTC
  AGCTACTGCTGGGTCTCTTCTGCACCAATGTGGCCTTCCCGCCGGATGCCTTGCGCATGCGCGCACCTGC
  CAGCCCCACTGCCGCAGATCCTGCCACCCCACAGGATCCACCTGGCCTCCCGCCTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226260 representing NM_001163689
  Red=Cloning site Green=Tags(s)
  
  MFPRETKWNISFAGCGFLGVYHIGVASCLREHAPFLVANATHIYGASAGALTATALVTGACLGEAGANII
  EVSKEARKRFLGPLHPSFNLVKTIRGCLLKTLPADCHERANGRLGISLTRVSDGENVIISHFSSKDELIQ
  ANVCSTFIPVYCGLIPPTLQGVRYVDGGISDNLPLYELKNTITVSPFSGESDICPQDSSTNIHELRVTNT
  SIQFNLRNLYRLSKALFPPEPMVLREMCKQGYRDGLRFLRRNGLLNQPNPLLALPPVVPQEEDAEEAAVV
  EERAGEEDQLQPYRKDRILEHLPARLNEALLEACVEPKDLMTTLSNMLPVRLATAMMVPYTLPLESAVSF
  TIRLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGGHLPSRLSEQVELRRAQSLPSVPLSCATYSEA
  LPNWVRNNLSLGDALAKWEECQRQLLLGLFCTNVAFPPDALRMRAPASPTAADPATPQDPPGLPPC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163689
• ORF Size: 1458 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163689.1, NP_001157161.1
• RefSeq Size: 2635 bp
• RefSeq ORF: 1461 bp
• Locus ID: 66853
• UniProt ID: Q8BJ56
• Cytogenetics: 7 F5
• MW: 54.1 kDa
• Gene Summary: Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed:15550674). Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.[UniProtKB/Swiss-Prot Function]