Tcf7l1 (NM_009332) Mouse Tagged ORF Clone

CAT#: MR226144

Tcf7l1 (Myc-DDK-tagged) - Mouse transcription factor 7-like 1 (T-cell specific, HMG box) (Tcf7l1), transcript variant 2

Product Images

Circular map for MR226144

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tcf7l1
• Synonyms: bHLHb21; Tcf-3; Tcf3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226144 representing NM_009332
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCAGCTCGGTGGTGGCCGCGGTGGCGGCGCCGGCGGGGGAGGCGGTGGCTCCGGAGCCGGGGCAA
  CCAGTGGAGGGGACGACCTCGGAGCGAACGACGAGCTGATCCCCTTCCAGGACGAGGGGGGCGAAGAGCA
  AGAGCCGAGCAGCGACAGCGCTTCGGCGCAGAGGGATCTGGATGAGGTCAAGTCGTCCCTGGTCAACGAA
  TCGGAGAATCAGAGCAGTAGCTCGGACTCCGAGGCGGAGAGGCGCCCGCAGCCCGCCCGGGACGCTTTCC
  AGAAGCCGCGCGACTATTTCGCCGAAGTGAGAAGGCCCCAGGATGGCGCTTTTTTTAAGGGACCTGCGTA
  TCCTGGGTACCCCTTCCTGATGATTCCAGACCTGAGCAGCCCGTACCTCTCCAACGGACCCCTGTCTCCT
  GGTGGAGCGCGCACCTACCTACAGATGAAATGGCCCCTCCTCGATGTCCCCTCCAGTGCTACAGTCAAGG
  ACACAAGGTCGCCATCTCCAGCACACTTGTCCAACAAAGTTCCTGTTGTTCAGCACCCTCATCACATGCA
  CCCGCTGACACCTCTCATCACCTACAGCAACGACCACTTCTCCCCTGCGTCCCCTCCCACGCATCTGTCC
  CCAGAGATCGACCCAAAGACAGGAATCCCCCGGCCCCCTCATCCATCTGAGCTGTCACCGTATTACCCAC
  TGTCTCCAGGAGCTGTCGGACAAATCCCCCATCCCCTCGGCTGGCTCGTCCCACAGCAAGGGCAGCCCAT
  GTACTCTCTCCCTCCGGGTGGTTTCCGGCATCCTTACCCTGCCCTCGCCATGAACGCTTCAATGTCCAGC
  CTGGTCTCAAGCCGGTTCCCACACATGGTGGCTCCTGCCCATCCTGGTCTGCCCACCTCAGGAATCCCCC
  ACCCTGCCATCGTCTCCCCCATTGTGAAGCAGGAGCCAGCAGCCCCCAGCCTGAGCCCTGCAGTGAGTGC
  GAAATCCCCAGTTACGGTGAAGAAGGAAGAGGAGAAGAAACCTCACGTGAAAAAGCCCCTGAATGCCTTC
  ATGTTGTATATGAAGGAGATGAGGGCCAAGGTGGTGGCCGAGTGTACCCTGAAGGAAAGTGCAGCCATTA
  ACCAAATCCTGGGAAGAAAGTGGCACAACCTGTCAAGAGAAGAACAGGCCAAATACTATGAGCTTGCCCG
  GAAAGAACGGCAGCTTCACGCGCAGCTCTACCCAACCTGGTCAGCCCGGGACAACTATGGGAAGAAGAAG
  AAGAGGAAGAGAGAAAAGCAGCTGTCACAGACACAGTCTCAGCAGCAAATCCAAGAGGCAGAGGGTGCTC
  TGGCCTCTAAGAGCAAGAAGCCATGCATTCAGTACCTGCCCCCTGAGAAGCCTTGTGATAGCCCTGCGTC
  TTCCCATGGCAGCACGCTGGACTCCCCTGCGACGCCCTCCGCAGCCTTGGCCTCACCAGCTGCCCCTGCG
  GCTACCCACTCCGAGCAAGCGCAGCCCCTGTCGCTCACCACCAAACCGGAGGCCCGGGCCCAGCTGGCTC
  TCCACTCCGCTGCCTTCCTGTCGGCTAAGGCGGCCGCCAGCAACTCCAGCCAGATGGGCAGCCAGCCCCC
  ACTCCTATCCAGGCCCTTGCCCTTGGGCTCTATGCCCGCCGCTCTGCTGACCTCTCCCCCTACTTTCCCA
  GCTACGCTCCATGCCCACCAGGCCCTCCCCGTGCTACAGGCCCAGCCTCTTTCCTTGGTCACCAAGTCTG
  CCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226144 representing NM_009332
  Red=Cloning site Green=Tags(s)
  
  MPQLGGGRGGGAGGGGGGSGAGATSGGDDLGANDELIPFQDEGGEEQEPSSDSASAQRDLDEVKSSLVNE
  SENQSSSSDSEAERRPQPARDAFQKPRDYFAEVRRPQDGAFFKGPAYPGYPFLMIPDLSSPYLSNGPLSP
  GGARTYLQMKWPLLDVPSSATVKDTRSPSPAHLSNKVPVVQHPHHMHPLTPLITYSNDHFSPASPPTHLS
  PEIDPKTGIPRPPHPSELSPYYPLSPGAVGQIPHPLGWLVPQQGQPMYSLPPGGFRHPYPALAMNASMSS
  LVSSRFPHMVAPAHPGLPTSGIPHPAIVSPIVKQEPAAPSLSPAVSAKSPVTVKKEEEKKPHVKKPLNAF
  MLYMKEMRAKVVAECTLKESAAINQILGRKWHNLSREEQAKYYELARKERQLHAQLYPTWSARDNYGKKK
  KRKREKQLSQTQSQQQIQEAEGALASKSKKPCIQYLPPEKPCDSPASSHGSTLDSPATPSAALASPAAPA
  ATHSEQAQPLSLTTKPEARAQLALHSAAFLSAKAAASNSSQMGSQPPLLSRPLPLGSMPAALLTSPPTFP
  ATLHAHQALPVLQAQPLSLVTKSAH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009332
• ORF Size: 1755 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009332.3
• RefSeq Size: 2742 bp
• RefSeq ORF: 1758 bp
• Locus ID: 21415
• UniProt ID: Q9Z1J1
• Cytogenetics: 6 32.27 cM
• MW: 62.9 kDa
• Gene Summary: Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis.[UniProtKB/Swiss-Prot Function]