Tcf7l1 (NM_009332) Mouse Untagged Clone

CAT#: MC219284

Tcf7l1 (untagged) - Mouse transcription factor 7-like 1 (T-cell specific, HMG box) (Tcf7l1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tcf7l1
• Synonyms: bHLHb21; Tcf-3; Tcf3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219284 representing NM_009332
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCAGCTCGGTGGTGGCCGCGGTGGCGGCGCCGGCGGGGGAGGCGGTGGCTCCGGAGCCGGGGCAA
  CCAGTGGAGGGGACGACCTCGGAGCGAACGACGAGCTGATCCCCTTCCAGGACGAGGGGGGCGAAGAGCA
  AGAGCCGAGCAGCGACAGCGCTTCGGCGCAGAGGGATCTGGATGAGGTCAAGTCGTCCCTGGTCAACGAA
  TCGGAGAATCAGAGCAGTAGCTCGGACTCCGAGGCGGAGAGGCGCCCGCAGCCCGCCCGGGACGCTTTCC
  AGAAGCCGCGCGACTATTTCGCCGAAGTGAGAAGGCCCCAGGATGGCGCTTTTTTTAAGGGACCTGCGTA
  TCCTGGGTACCCCTTCCTGATGATTCCAGACCTGAGCAGCCCGTACCTCTCCAACGGACCCCTGTCTCCT
  GGTGGAGCGCGCACCTACCTACAGATGAAATGGCCCCTCCTCGATGTCCCCTCCAGTGCTACAGTCAAGG
  ACACAAGGTCGCCATCTCCAGCACACTTGTCCAACAAAGTTCCTGTTGTTCAGCACCCTCATCACATGCA
  CCCGCTGACACCTCTCATCACCTACAGCAACGACCACTTCTCCCCTGCGTCCCCTCCCACGCATCTGTCC
  CCAGAGATCGACCCAAAGACAGGAATCCCCCGGCCCCCTCATCCATCTGAGCTGTCACCGTATTACCCAC
  TGTCTCCAGGAGCTGTCGGACAAATCCCCCATCCCCTCGGCTGGCTCGTCCCACAGCAAGGGCAGCCCAT
  GTACTCTCTCCCTCCGGGTGGTTTCCGGCATCCTTACCCTGCCCTCGCCATGAACGCTTCAATGTCCAGC
  CTGGTCTCAAGCCGGTTCCCACACATGGTGGCTCCTGCCCATCCTGGTCTGCCCACCTCAGGAATCCCCC
  ACCCTGCCATCGTCTCCCCCATTGTGAAGCAGGAGCCAGCAGCCCCCAGCCTGAGCCCTGCAGTGAGTGC
  GAAATCCCCAGTTACGGTGAAGAAGGAAGAGGAGAAGAAACCTCACGTGAAAAAGCCCCTGAATGCCTTC
  ATGTTGTATATGAAGGAGATGAGGGCCAAGGTGGTGGCCGAGTGTACCCTGAAGGAAAGTGCAGCCATTA
  ACCAAATCCTGGGAAGAAAGTGGCACAACCTGTCAAGAGAAGAACAGGCCAAATACTATGAGCTTGCCCG
  GAAAGAACGGCAGCTTCACGCGCAGCTCTACCCAACCTGGTCAGCCCGGGACAACTATGGGAAGAAGAAG
  AAGAGGAAGAGAGAAAAGCAGCTGTCACAGACACAGTCTCAGCAGCAAATCCAAGAGGCAGAGGGTGCTC
  TGGCCTCTAAGAGCAAGAAGCCATGCATTCAGTACCTGCCCCCTGAGAAGCCTTGTGATAGCCCTGCGTC
  TTCCCATGGCAGCACGCTGGACTCCCCTGCGACGCCCTCCGCAGCCTTGGCCTCACCAGCTGCCCCTGCG
  GCTACCCACTCCGAGCAAGCGCAGCCCCTGTCGCTCACCACCAAACCGGAGGCCCGGGCCCAGCTGGCTC
  TCCACTCCGCTGCCTTCCTGTCGGCTAAGGCGGCCGCCAGCAACTCCAGCCAGATGGGCAGCCAGCCCCC
  ACTCCTATCCAGGCCCTTGCCCTTGGGCTCTATGCCCGCCGCTCTGCTGACCTCTCCCCCTACTTTCCCA
  GCTACGCTCCATGCCCACCAGGCCCTCCCCGTGCTACAGGCCCAGCCTCTTTCCTTGGTCACCAAGTCTG
  CCCACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009332
• Insert Size: 1758 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009332.2, NP_033358.2
• RefSeq Size: 2742 bp
• RefSeq ORF: 1758 bp
• Locus ID: 21415
• UniProt ID: Q9Z1J1
• Cytogenetics: 6 32.27 cM
• Gene Summary: Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.