Fbln5 (NM_011812) Mouse Tagged ORF Clone

CAT#: MR226107

Fbln5 (Myc-DDK-tagged) - Mouse fibulin 5 (Fbln5)

Product Images

Circular map for MR226107

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fbln5
• Synonyms: A55; DANCE; EVEC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226107 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGATTAAAAAGGATACTCACTGTTACCATCTTGGCACTCTGGCTTCCACATCCTGGGAATGCAC
  AGCAGCAGTGCACAAACGGCTTTGACCTGGACCGCCAGTCAGGACAGTGTCTAGATATTGATGAATGCCG
  GACCATCCCTGAGGCTTGTCGTGGGGACATGATGTGTGTCAACCAGAATGGCGGGTATTTGTGCATCCCT
  CGAACCAACCCAGTGTATCGAGGGCCTTACTCAAATCCCTACTCTACATCCTACTCAGGCCCATACCCAG
  CAGCGGCCCCACCAGTACCAGCTTCCAACTACCCCACGATTTCAAGGCCTCTTGTCTGCCGCTTTGGGTA
  TCAGATGGATGAAGGCAACCAGTGTGTGGATGTGGACGAGTGTGCAACAGACTCACACCAGTGCAACCCT
  ACCCAGATCTGTATCAACACTGAAGGAGGTTACACCTGCTCCTGCACCGATGGGTACTGGCTTCTGGAAG
  GGCAGTGCCTAGATATTGATGAATGTCGCTATGGTTACTGCCAGCAGCTCTGTGCAAATGTTCCAGGATC
  CTATTCCTGTACATGCAACCCTGGTTTCACCCTCAACGACGATGGAAGGTCTTGCCAAGATGTGAACGAG
  TGCGAAACTGAGAATCCCTGTGTTCAGACCTGTGTCAACACCTATGGCTCTTTCATCTGCCGCTGTGACC
  CAGGATATGAACTTGAGGAAGATGGCATTCACTGCAGTGATATGGACGAGTGCAGCTTCTCCGAGTTCCT
  CTGTCAACACGAGTGTGTGAACCAGCCGGGCTCATACTTCTGCTCGTGCCCTCCAGGCTACGTCCTGTTG
  GATGATAACCGAAGCTGCCAGGATATCAATGAATGTGAGCACCGAAACCACACGTGTACCTCACTGCAGA
  CTTGCTACAATCTACAAGGGGGCTTCAAATGTATTGATCCCATCAGCTGTGAGGAGCCTTATCTGCTGAT
  TGGTGAAAACCGCTGTATGTGTCCTGCTGAGCACACCAGCTGCAGAGACCAGCCATTCACCATCCTGTAT
  CGGGACATGGATGTGGTGTCAGGACGCTCCGTTCCTGCTGACATCTTCCAGATGCAAGCAACAACCCGAT
  ACCCTGGTGCCTATTACATTTTCCAGATCAAATCTGGCAACGAGGGTCGAGAGTTCTATATGCGGCAAAC
  AGGGCCTATCAGTGCCACCCTGGTGATGACACGCCCCATCAAAGGGCCTCGGGACATCCAGCTGGACTTG
  GAGATGATCACTGTCAACACTGTCATCAACTTCAGAGGCAGCTCCGTGATCCGACTGCGGATATATGTGT
  CGCAGTATCCGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226107 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPGLKRILTVTILALWLPHPGNAQQQCTNGFDLDRQSGQCLDIDECRTIPEACRGDMMCVNQNGGYLCIP
  RTNPVYRGPYSNPYSTSYSGPYPAAAPPVPASNYPTISRPLVCRFGYQMDEGNQCVDVDECATDSHQCNP
  TQICINTEGGYTCSCTDGYWLLEGQCLDIDECRYGYCQQLCANVPGSYSCTCNPGFTLNDDGRSCQDVNE
  CETENPCVQTCVNTYGSFICRCDPGYELEEDGIHCSDMDECSFSEFLCQHECVNQPGSYFCSCPPGYVLL
  DDNRSCQDINECEHRNHTCTSLQTCYNLQGGFKCIDPISCEEPYLLIGENRCMCPAEHTSCRDQPFTILY
  RDMDVVSGRSVPADIFQMQATTRYPGAYYIFQIKSGNEGREFYMRQTGPISATLVMTRPIKGPRDIQLDL
  EMITVNTVINFRGSSVIRLRIYVSQYPF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011812
• ORF Size: 1347 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011812.4
• RefSeq Size: 5893 bp
• RefSeq ORF: 1347 bp
• Locus ID: 23876
• UniProt ID: Q9WVH9
• Cytogenetics: 12 E
• MW: 50.2 kDa
• Gene Summary: Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN (By similarity). Stabilizes and organizes elastic fibers in the skin, lung and vasculature. Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Vascular ligand for integrin receptors which may play a role in vascular development and remodeling (PubMed:11805835). May act as an adapter that mediates the interaction between FBN1 and ELN (By similarity).[UniProtKB/Swiss-Prot Function]