Cdk5rap2 (NM_145990) Mouse Tagged ORF Clone

CAT#: MR226032

Cdk5rap2 (Myc-DDK-tagged) - Mouse CDK5 regulatory subunit associated protein 2 (Cdk5rap2)

Product Images

Circular map for MR226032

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdk5rap2
• Synonyms: 2900018K03Rik; an; mKIAA1633
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226032 representing NM_145990
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCGGGGATGGAAGAGGAAGGCGCCCTGCCTGGGACCCTCAGCGGTTGCAGTGGCCTTCACCCTG
  TTCTGCCAAGTGACCTGGATGTCATCAGTGACACTTCTGGCTTGGGAAATGGAGTGCTCCCAAGCATGTC
  AGAAGAAAAGGTTTCTCCCACCAGAGCCCGGAACATGAAGGACTTTGAAAACCAAATCACTGAGCTGAAG
  AAGGAGAACTTCAATCTAAAGCTCCGGATCTACTTCCTGGAGGAGAGAATCCAGCAGGAGTTTGCTGGCC
  CCACCGAGCACATCTACAAGACGAACATTGAACTCAAGGTGGAAGTGGAAAGTCTGAAGCGCGAGCTCCA
  GGAGAAAGATCAGTTGCTCGTCAAAGCCTCCAAAGCGGTGGAGAGCCTAGCAGAACGGGGTGGTTCTGAA
  GTCCAGCGGGTGAAAGAAGATGCAAGGAAGAAGGTGCAGCAGGTGGAAGACCTCCTAACGAAAAGGATAC
  ATCTTCTAGAAGAAGATGTGAAAGCCGCCCAGGCAGAACTGGAGAAGGCCTTTGCGGGGACAGAAACAGA
  GAAGGCTCTTCGGCTCAGCTTAGAAAGCAAGCTCTCGGCGATGAAGAAGATGCAGGAGGGGGACCTGGAG
  ATGACTCTGGCTCTGGAAGAGAAGGACAGACTGATTGAGGAGTTGAAGCTGTCTTTGAAGAGCAAAGAAG
  CTTTAATTCAGTGCCTTAAAGAGGAAAAATCTCAGATGGCATCTCCTGACGAGAACGTGTCATCTGGAGA
  ACTCCGAGGACTTTCTGCCACTCTGAGGGAAGAAAAAGAGAGAGACGCTGAGGAATGGCAGAAGGAGAGA
  AACCACTTTGAAGAGAGGATCCAGGCACTTCAGGAGGACCTGAGAGAGAAGGAAAGAGAAATTGCTACAG
  AGAAGAAAAATAACTTAAAGAGTTATAAAGCCATTCAGGGTCTAACCATGGCATTAAAATCAAAGGAAAG
  AGAGGTTGAAGAACTTGATTCCAAAATCAAAGAGGTCACTACGGACTCCACCAAAGGCAGAGAGGATCCC
  TTAAAAACCCAGATCCCCAGATTCCAGCTAAGAGAGGGGTCTGAGGACTGTGAAGCTGCGCTGGTAGAGA
  AGGAAGCCCTATTGGCCAAGCTACACTCAGAAAATGTCACAAAGAGCACTGAGAACCATAGGTTGCTCAG
  GAATGTTAAAAAGGTCACCCAGGAGTTGAATGACTTGAAGAAGGAGAAGTTGAGACTAGAGCAGGCCCTG
  GAGGAAGCCCACCAAGAGGGGAACAGAGGAGCCCGAACCATTCACGACCTTAGAAATGAAGTTGAGAAAC
  TACGCAAGGAAGTGAGTGAGAGAGAGAAGGCTGTGGAAAAACATTACAAGAGTCTTCCAGGTGAAAGCAA
  GACAAAGTTTCATACTCAAGAACAAGTGGTCAGAAGTCTGACAGGCAGTGGCAGTCAGGAGGACCTGCTG
  CTTCAGAAATCCAATGAAAAAGATTTGGAAGCAATACAGCAGAACTGCTATTTAATGACTGCAGAGGAGC
  TCAAGTTTGGGAGCGATGGCTTAATAACAGAAAAGTGTTCTCAGCAGTCACCAGACAGCAAACTCATCTT
  CTCCAAGGAAAAGCAACAATCAGAGTATGAAGGGCTGACTGGGGACTTAAAAGCAGAACAGAACATCTAT
  GCCCATCTGGCAAAGACTCAGGACACAGACAGTGTCAGCAACCTGCAGGCTGAGCTGAAAGAGGTCCTTG
  CCCTTCGGAAGCAGCTGGAGCAGGATGTTCTTGCCTACCGGAACCTGCAGAAGGCCCTGCAGGAGCAGCT
  CAGTGAGATTCGGAGTCGAGAAGAAGAGCCGTTTTCATTTTATAGCGATCAAACATCGTATCTGAGTATT
  TGCCTTGAAGAGCACAATCAGTTCCAATTGGAGCATTTTTCTCAAGAAGAACTCAAGAAAAAGGTCAGTG
  ACCTCATACAGCTTGTGAAGGACCTGCACACAGACAACCAGCACCTGAAGAAAACCATTTTTGATCTCTC
  TTCTGTGGGTTTCCAGGGAAGTGACAGACTTGAGTTAACAAAACAAGAAGAGCTTGTGGCTAGCAAGGAG
  GATGAGGACACACTCAAATTTGAAGCGGACGTTGAGACTCCTTTCCAGAGTGACCAGCATTTGGAGCAAA
  GTCGTGAGATCATGGAGGACTATGCTGAGGGTGGCTGCAAAAGTGGATATGGAAGGCACATGGATTCCAA
  TATTTTAGGTCATGATGGGGCCCAAACTCCTGGTGCCTCTGAAGAGCACACCCTAGAAGATGAGCTCCTG
  GGCCTGCTTGCCACTCTCTTCAGTAAGAAGGCCACACCGTTGCTGGAATCCAGGCCAGATCTTCTGAAAG
  CACTCGGTGCCCTACTTTTGGAACGCATATGCTTGGCAGAGCAGGGTCGTCCTGGAGATCACCTTGATAG
  TAAAACTGAGAAGGCACTTCAGCAAGTAGCTGTTCAGTTGAGAGATGAACTTGGCCATTCCTTCCCTGCC
  AACTCGTTCTCCAAGTCATACAATGAAGTCAAGTCAATGTGGGGAAATTGGCTGGTAAAGACAGGAGACG
  AAGACACGGTGGAGCTGAAGAGTGTTTCAGTGCAGACCATGGCCATAGAGGACACCCCGCATGGATTCAA
  ACCTCAAAGCAAGAGAGATGCTTGGGCAGAGAAACAAGAAGAGGCCATTTTCAGCACTGAGCTTGAGTCA
  GAGGCGTTGGGTGAGATGCCAGGGCAACAGGCCACTCACCTCTCATTCCCCAGTGCCATCAACCCGGATG
  CCGAGAAGACGGGCTTGTTGATCCAATTAAAGACACCTGAGTTATTGGAAAATTTATATAATCTTCCTGC
  TTCGCCAGAGGTGGTGGTGGCTCAACTCCAAGGCCAGGTTCTAGAGCTGCAGAGGGAGCTGAAAGAATTC
  AAAACCCGCAACAAGCAACTCCATGAGAAGCTAATTCTGGCAGAGGCAATGATGGAGGGGCTGCCGGTGC
  CCAACTCTGCGCTGGTGAATGTGCCTGCAGCCCAGGCTGTTGTGAGAACAGCCTTCCAGGACAACCCAGG
  CGAGCAAGAAGGACCTGAAACCACACAGTCTGCTGGGAGAGACAAGGACATGGACAGTGACCAATATACA
  AGCTTTGAGATCGATTCTGAAATCTGTCCACCTGATGACCTTGCCTTATTACCAGCATGCAAAGAGAATC
  TTGAAGACCTCCTAGGCCCATCCTCCATAGCCACTTACCTGGATTCCAAGAGTCAGCTCTCTGTTAAAGT
  CAGCGTGAATGGAACTGACCAGTCTGAGAATATCAATATCCCTGATGACACAGAAGACTTAAAACAGAAA
  ATCCACGACTTGCAAACCGAGCTGGAAGGCTACCGGAACATCATTGTCCAGCTACTGAAGCACTCTCAGT
  GCAGTGAGGCTATTATCACAGTTTTGTGTGGGACAGAAGGGGCCCAAGATGGCTTGAACAAGCCTAAGGG
  CCATATTGATGAGGAAGAGATGACATTTTCAAGTTTGCACCAAGTACGATATGTGAAGCATATGAAAATC
  CTCCGTCCACTAACCCCGGAGATGATTGATGGCAAGATGCTAGAGAGCCTCAAGCAGCAGCTGGTGGACC
  AGGAGCAGGAGCTACAGAAGGAGCAGGACTTGAACTTGGAACTTTTCGGTGAAATCCATGACCTGCAGAA
  CAAGTTCCAAGACCTCTCGCCATCAAGATACGATTCATTGGTTCAGTCCCAAGCGAGGGAGCTCTCCCTT
  CAGCGACAGCAAATTAAGGATAGCCATGGCATCTGTGTCATTTACCGTCAACACATGAGCACCATGATCA
  AGGCGTTTGAGGAGCTTCTGCAGGCCAGTGATGTGGACAGTTGCGTGGCCGAGGGCTTCCGGGAGCAGCT
  GACACAGTGTGCTGGACTGCTAGAGCAACTGGAGAGGCTGTTTCTCCATGGAAAATCAGCCAGAGTGGAA
  CCACATCCCCAGAATGAACTGCTCAAGGGGTTAAGGACTGTGGAAGGCAACTTACCCTACCACCATCTCC
  TGCCTGAATCTCCTGAGCCTTCTGCCTCCCATGCTCTGTCAGATGATGAAATGTCTGAGAAGTCCTTCCT
  GTCTAGAGACCCGAAGCCAGACAGCGATACTGAGAAGTACCCAGCAATGGCGAGCCATTTTCCTCAAGAC
  TTACTAATGGAACACATACAGGAAATTCGAACTTTGAGAAAGCATTTGGAAGAATCTATTAAAACAAATG
  AGAAGCTCCGGAAACAGCTGGAACGGCAGGGGTCTGAGACTGACCAAGGCTCCAGAAATGTTTCTGCTTG
  TGGCTTAGCACTGCACAGCTCTCTGACGTCAGAGATACATTTCCTGAGGAAGCAGAACGAGGCCCTCAGT
  ATGATGCTCGAGAAGGGGTCCAAAGATAAACAGAAAGAGAGTGAAAAATTACGTGAGTCCCTGGCTAGGA
  AAGCTGAGAGTCTTGAGCAACTTCAGCTAGAGTACACCAGTGTAAGGGAAGAAAATGAAAGGTTACAGAG
  AGACATCATTGAGAAGGAGAGACACAACCAGGAGCTGACTGAGGAGGTCTGCAGCAGCCGCCAGGAACTG
  AGCAGGGTGCAGGAAGAGGCGAAGTCAAGACAGCAGCTGCTCTCACAGAAGGACAAGCTGCTTCAGTCCC
  TCCAAATGGAGCTCAAGGTGTATGAGAAACTGGCCGAGGAACATCCGAGGCTACAGCAAGATGGCAGCAA
  ATGTCCTGAGGCCAGCGACAATTCATTTGATCTATTTGAATCCACCCAGGCAATGGCACCTAAATCAGCT
  TCTGAGACTCCTCTGCTCTCAGGAACTGATGTTGACTCGCTCTCCTGTGACAGTACCAGTTCTGCCACCA
  GCCCTACTAGCATGCCCTGCCTGGTTGCTGGCCACCACATGTGGGCCAGCAAGAGTGGCCACCATATGCT
  GGGCCTGATTGAGGACTATGATGCCCTCTATAAGCAGATCAGCTGGGGCCAGACACTGCTTGCCAAGATG
  GATGTTCAAACCCAAGAGGCTCTGAGCCCCACAAGTCACAAGCTGGGACCAAAGGGTTCTTCATCCGTGC
  CTCTGAGCAAGTTTCTTTCCAGTATGAACACAGCCAAGCTGGTCCTGGAAAAAGCATCCAGGTTGCTGAA
  GCTCTTCTGGAGGGTCTCAGTCCCCACCAACGGCCAGTGTTCCCTTCACTGTGAGCAGATTGGAGAAATG
  AAGGCAGAGAACACGAAACTACACAAAAAATTGTTTGAACAAGAAAAGAAGCTGCAGAACACAGCAAAAC
  TATTGCAGCAGAGCAAGCACCAGGAGAAAGTCATCTTTGATCAGTTGGTCATCACCCACCAAGTCCTTCG
  GAAGGCCAGGGGAAACCTGGAGCTCAGGCCTGGGGCCACCCGTCCAGGAGCATCCAGTCCCAGCAGACCG
  GGCTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226032 representing NM_145990
  Red=Cloning site Green=Tags(s)
  
  MDSGMEEEGALPGTLSGCSGLHPVLPSDLDVISDTSGLGNGVLPSMSEEKVSPTRARNMKDFENQITELK
  KENFNLKLRIYFLEERIQQEFAGPTEHIYKTNIELKVEVESLKRELQEKDQLLVKASKAVESLAERGGSE
  VQRVKEDARKKVQQVEDLLTKRIHLLEEDVKAAQAELEKAFAGTETEKALRLSLESKLSAMKKMQEGDLE
  MTLALEEKDRLIEELKLSLKSKEALIQCLKEEKSQMASPDENVSSGELRGLSATLREEKERDAEEWQKER
  NHFEERIQALQEDLREKEREIATEKKNNLKSYKAIQGLTMALKSKEREVEELDSKIKEVTTDSTKGREDP
  LKTQIPRFQLREGSEDCEAALVEKEALLAKLHSENVTKSTENHRLLRNVKKVTQELNDLKKEKLRLEQAL
  EEAHQEGNRGARTIHDLRNEVEKLRKEVSEREKAVEKHYKSLPGESKTKFHTQEQVVRSLTGSGSQEDLL
  LQKSNEKDLEAIQQNCYLMTAEELKFGSDGLITEKCSQQSPDSKLIFSKEKQQSEYEGLTGDLKAEQNIY
  AHLAKTQDTDSVSNLQAELKEVLALRKQLEQDVLAYRNLQKALQEQLSEIRSREEEPFSFYSDQTSYLSI
  CLEEHNQFQLEHFSQEELKKKVSDLIQLVKDLHTDNQHLKKTIFDLSSVGFQGSDRLELTKQEELVASKE
  DEDTLKFEADVETPFQSDQHLEQSREIMEDYAEGGCKSGYGRHMDSNILGHDGAQTPGASEEHTLEDELL
  GLLATLFSKKATPLLESRPDLLKALGALLLERICLAEQGRPGDHLDSKTEKALQQVAVQLRDELGHSFPA
  NSFSKSYNEVKSMWGNWLVKTGDEDTVELKSVSVQTMAIEDTPHGFKPQSKRDAWAEKQEEAIFSTELES
  EALGEMPGQQATHLSFPSAINPDAEKTGLLIQLKTPELLENLYNLPASPEVVVAQLQGQVLELQRELKEF
  KTRNKQLHEKLILAEAMMEGLPVPNSALVNVPAAQAVVRTAFQDNPGEQEGPETTQSAGRDKDMDSDQYT
  SFEIDSEICPPDDLALLPACKENLEDLLGPSSIATYLDSKSQLSVKVSVNGTDQSENINIPDDTEDLKQK
  IHDLQTELEGYRNIIVQLLKHSQCSEAIITVLCGTEGAQDGLNKPKGHIDEEEMTFSSLHQVRYVKHMKI
  LRPLTPEMIDGKMLESLKQQLVDQEQELQKEQDLNLELFGEIHDLQNKFQDLSPSRYDSLVQSQARELSL
  QRQQIKDSHGICVIYRQHMSTMIKAFEELLQASDVDSCVAEGFREQLTQCAGLLEQLERLFLHGKSARVE
  PHPQNELLKGLRTVEGNLPYHHLLPESPEPSASHALSDDEMSEKSFLSRDPKPDSDTEKYPAMASHFPQD
  LLMEHIQEIRTLRKHLEESIKTNEKLRKQLERQGSETDQGSRNVSACGLALHSSLTSEIHFLRKQNEALS
  MMLEKGSKDKQKESEKLRESLARKAESLEQLQLEYTSVREENERLQRDIIEKERHNQELTEEVCSSRQEL
  SRVQEEAKSRQQLLSQKDKLLQSLQMELKVYEKLAEEHPRLQQDGSKCPEASDNSFDLFESTQAMAPKSA
  SETPLLSGTDVDSLSCDSTSSATSPTSMPCLVAGHHMWASKSGHHMLGLIEDYDALYKQISWGQTLLAKM
  DVQTQEALSPTSHKLGPKGSSSVPLSKFLSSMNTAKLVLEKASRLLKLFWRVSVPTNGQCSLHCEQIGEM
  KAENTKLHKKLFEQEKKLQNTAKLLQQSKHQEKVIFDQLVITHQVLRKARGNLELRPGATRPGASSPSRP
  GS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145990
• ORF Size: 5466 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145990.4, NP_666102.2
• RefSeq Size: 11840 bp
• RefSeq ORF: 5469 bp
• Locus ID: 214444
• UniProt ID: Q8K389
• Cytogenetics: 4 C2
• MW: 205.9 kDa
• Gene Summary: Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion (PubMed:20627074). Involved in regulation of mitotic spindle orientation (PubMed:20460369). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Required for the recruitment of AKAP9 to centrosomes (By similarity). Plays a role in neurogenesis (PubMed:20471352). Contrary to higher mammalian orthologs, including human, chimpanzee, bovine and dog, does not interact with EB1/MAPRE1, therefore its function in the regulation of microtubule dynamics is unclear (PubMed:19553473).[UniProtKB/Swiss-Prot Function]