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Polr2a (NM_009089) Mouse Tagged ORF Clone
CAT#: MR225735
- TrueORF®
Polr2a (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide A (Polr2a).
ORF Plasmid: tGFP
"NM_009089" in other vectors (2)
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Polr2a |
| Synonyms | 220kDa; Rpb1; Rpo2-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225735 representing NM_009089.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCACGGGGGTGGCCCCCCCTCCGGGGACAGCGCATGCCCGCTGCGCACCATCAAGAGAGTGCAGTTC GGAGTCCTGAGTCCGGATGAATTGAAGCGGATGTCTGTGACAGAGGGTGGTATCAAATACCCAGAAACA ACGGAGGGAGGTCGCCCCAAACTTGGGGGACTAATGGATCCACGGCAGGGGGTGATTGAGCGGACTGGC CGCTGCCAAACATGTGCAGGAAACATGACCGAGTGTCCTGGCCACTTTGGCCACATCGAATTGGCCAAA CCTGTGTTTCATGTGGGCTTCCTGGTGAAGACAATGAAGGTTTTGCGCTGTGTCTGCTTCTTCTGCTCC AAACTGCTTGTAGATTCTAATAACCCGAAGATTAAGGACATCCTGGCCAAATCTAAGGGGCAGCCAAAG AAACGGCTTACGCATGTCTATGACCTGTGCAAGGGCAAAAACATCTGTGAAGGTGGAGAGGAGATGGAC AACAAGTTTGGTGTGGAGCAGCCGGAGGGTGATGAGGATCTGACCAAAGAGAAGGGCCATGGCGGCTGT GGGCGGTACCAACCCCGAATCCGGCGCTCTGGCCTAGAGTTGTATGCTGAATGGAAGCATGTTAATGAG GACTCTCAGGAGAAAAAGATCTTGCTGAGTCCAGAACGAGTGCATGAAATCTTCAAACGCATCTCAGAT GAGGAGTGTTTTGTGTTGGGCATGGAGCCTCGCTATGCCCGGCCTGAGTGGATGATTGTCACAGTGTTG CCTGTGCCTCCTCTCTCAGTGAGACCTGCTGTGGTGATGCAGGGTTCTGCTCGAAACCAGGATGATCTG ACTCACAAACTGGCTGACATTGTGAAGATCAACAATCAGCTGCGGCGGAACGAGCAGAATGGTGCAGCT GCCCATGTCATAGCAGAAGATGTAAAACTTCTCCAGTTCCATGTGGCCACCATGGTGGACAATGAACTG CCCGGCTTACCCCGTGCCATGCAGAAGTCTGGCCGTCCCCTCAAGTCCCTGAAGCAACGGTTGAAGGGC AAAGAAGGACGGGTTCGAGGGAATCTAATGGGCAAGCGAGTGGACTTCTCAGCCCGCACTGTCATCACC CCTGACCCTAACCTATCCATTGACCAAGTGGGTGTGCCCCGCTCCATTGCTGCCAACATGACCTTTGCA GAGATTGTCACACCCTTCAATATTGACAGACTTCAGGAATTAGTACGCAGGGGAAACAGCCAGTATCCA GGAGCCAAGTACATCATCCGCGATAATGGCGATCGAATTGACTTGCGTTTCCATCCTAAGCCCAGTGAC CTTCATCTGCAGACTGGCTATAAGGTGGAACGGCACATGTGTGATGGGGACATTGTTATCTTCAATCGT CAGCCAACTTTGCACAAAATGTCCATGATGGGGCATCGGGTCCGAATCCTCCCCTGGTCTACTTTTCGG TTGAATCTTAGTGTGACAACTCCATACAATGCAGACTTTGATGGAGATGAGATGAATTTGCATCTGCCA CAGTCTCTGGAAACACGGGCAGAGATCCAAGAGTTGGCTATGGTGCCTCGAATGATTGTCACCCCCCAG AGCAATCGACCTGTCATGGGTATTGTGCAAGACACACTCACGGCAGTACGCAAATTCACTAAGAGAGAT GTCTTCCTGGAGCGGGGTGAAGTCATGAACCTCTTAATGTTTCTGTCCACGTGGGATGGCAAGGTCCCA CAACCAGCTATCCTCAAGCCCCGGCCCCTGTGGACAGGCAAGCAGATCTTCTCCCTCATCATACCTGGA CACATCAATTGTATCCGTACCCACAGCACCCATCCTGATGATGAAGATAGTGGCCCTTACAAGCACATC TCTCCTGGGGACACCAAAGTTGTAGTGGAGAATGGGGAGCTTATCATGGGCATCCTTTGTAAGAAGTCC CTGGGCACCTCAGCAGGCTCGCTGGTCCATATCTCCTACCTGGAGATGGGGCATGACATCACCCGCCTC TTCTACTCCAACATTCAGACTGTCATTAACAACTGGCTTCTCATCGAGGGTCATACCATTGGCATTGGG GACTCCATTGCAGATTCAAAGACTTACCAGGACATTCAGAACACTATTAAGAAAGCTAAACAAGATGTA ATAGAGGTCATTGAGAAGGCTCATAACAATGAGCTAGAACCCACTCCAGGAAACACATTGCGTCAGACA TTTGAGAATCAAGTGAATCGTATTCTCAATGATGCTCGAGACAAAACTGGCTCCTCTGCACAGAAATCC CTCTCTGAATATAACAACTTCAAGTCTATGGTGGTGTCTGGAGCCAAGGGTTCCAAGATCAACATCTCC CAGGTCATTGCTGTTGTCGGGCAGCAGAATGTAGAGGGCAAGCGGATCCCATTTGGATTCAAGCATCGG ACTCTTCCTCACTTTATCAAGGATGATTATGGTCCTGAGAGCCGAGGCTTTGTAGAAAATTCATACTTG GCTGGTCTCACACCCACTGAGTTCTTCTTCCATGCCATGGGAGGACGAGAAGGTCTCATTGACACGGCT GTTAAGACTGCTGAGACTGGGTATATTCAGCGAAGGCTGATAAAGTCCATGGAGTCGGTGATGGTGAAA TATGATGCAACTGTGAGGAACTCCATCAACCAGGTGGTACAGCTGCGCTATGGAGAGGACGGCCTGGCA GGGGAGAGTGTTGAGTTCCAGAATCTGGCTACACTTAAACCTTCTAATAAAGCTTTTGAGAAGAAGTTC CGTTTTGATTATACCAATGAGAGGGCCCTGCGGCGCACCCTTCAGGAGGACCTGGTAAAGGATGTGCTG AGCAATGCCCATATACAGAATGAGCTGGAACGGGAATTTGAACGGATGCGTGAAGATCGGGAAGTGCTC AGGGTCATCTTCCCAACTGGTGACAGCAAGGTGGTCCTCCCTTGTAACCTTCTGCGGATGATCTGGAAT GCTCAGAAAATCTTCCACATCAACCCTCGCCTTCCCTCTGACCTGCATCCCATCAAAGTGGTAGAGGGA GTCAAAGAATTGAGCAAGAAGTTGGTAATTGTGAATGGAGATGACCCACTAAGTCGGCAGGCACAGGAG AATGCCACATTGCTCTTCAACATCCACCTAAGATCCACACTCTGCTCTCGCCGCATGGCTGAGGAGTTT CGGCTGAGTGGAGAGGCCTTTGACTGGCTGCTTGGGGAGATAGAGTCCAAGTTCAACCAAGCCATTGCC CATCCTGGAGAAATGGTGGGAGCTCTGGCTGCTCAGTCTCTTGGAGAACCTGCCACTCAGATGACCCTG AACACTTTCCACTATGCTGGTGTATCTGCCAAGAATGTGACGCTGGGTGTGCCTCGACTTAAGGAGCTT ATCAACATTTCCAAGAAGCCAAAGACTCCTTCACTCACTGTCTTCCTGTTAGGTCAATCTGCACGAGAT GCTGAAAGAGCCAAGGACATTCTTTGCCGCTTGGAACATACAACATTGAGGAAGGTGACTGCCAATACA GCCATCTACTATGACCCTAACCCCCAGAGCACAGTGGTGGCAGAAGATCAGGAGTGGGTGAATGTGTAC TATGAGATGCCCGACTTTGACGTGGCCCGGATATCTCCCTGGCTGCTGCGTGTGGAGCTCGACCGAAAG CACATGACTGACCGGAAGCTTACCATGGAACAGATTGCTGAGAAAATCAATGCAGGTTTTGGCGATGAT TTGAATTGTATCTTCAACGATGATAATGCAGAGAAGCTGGTCCTTCGAATCCGCATCATGAACAGTGAT GAAAACAAGATGCAAGAGGAGGAAGAGGTGGTGGATAAAATGGATGATGATGTCTTCCTGCGATGCATT GAGTCCAACATGCTGACAGATATGACCCTGCAGGGTATCGAGCAGATCAGCAAGGTGTACATGCACTTA CCTCAGACAGACAACAAGAAGAAGATCATCATCACAGAGGATGGGGAGTTCAAGGCCCTGCAGGAATGG ATCTTAGAGACGGATGGTGTGAGCCTGATGCGGGTGCTGAGTGAGAAGGATGTAGACCCTGTGCGCACC ACGTCCAATGATATTGTGGAGATCTTCACGGTACTGGGCATTGAGGCTGTGCGGAAGGCTCTGGAGCGA GAACTGTACCATGTCATCTCCTTTGATGGTTCTTATGTCAATTATCGGCATTTGGCGCTCCTGTGTGAC ACTATGACATGCCGTGGCCACTTGATGGCCATCACTCGTCATGGGGTCAACCGCCAGGACACTGGACCG CTCATGAAATGCTCCTTTGAGGAAACGGTGGATGTCCTTATGGAAGCAGCAGCACATGGGGAGAGTGAC CCGATGAAGGGAGTCTCTGAGAATATTATGCTGGGCCAGCTCGCTCCAGCTGGTACTGGCTGTTTTGAC CTCCTGCTTGATGCTGAGAAGTGCAAATACGGCATGGAAATCCCCACCAATATCCCTGGCCTGGGGGCT GCTGGACGGAGCGGGATGACCCCAGGAGCGGCCGGCTTCTCTCCCAGTGCTGCATCTGATGCCAGTGGC TTCAGCCCAGGTTACTCCCCTGCATGGTCTCCCACACCAGGCTCTCCGGGCTCCCCTGGACCCTCAAGC CCATACATCCCCTCACCAGGTGGTGCTATGTCTCCCAGCTACTCACCGACATCACCAGCCTATGAGCCA CGCTCCCCTGGGGGCTATACACCCCAGAGCCCCTCCTACTCCCCTACTTCACCTTCCTACTCCCCAACC TCTCCATCTTACTCTCCAACCAGTCCCAACTACAGCCCTACCTCTCCTAGCTACTCGCCCACCTCCCCT AGCTACTCGCCAACCTCTCCTTCCTACTCCCCCACCTCTCCAAGCTATTCCCCAACCTCTCCTAGCTAC TCCCCAACCTCTCCAAGCTATTCTCCAACATCACCTAGCTATTCTCCAACTTCTCCCAGCTACTCACCG ACATCTCCTAGCTACTCCCCAACTTCTCCCAGCTACTCACCAACTTCACCAAGCTATTCTCCCACCTCC CCCAGCTACTCACCGACATCTCCCAGCTACTCACCAACTTCTCCAAGCTACTCACCAACTTCTCCAAGC TACTCACCCACCAGCCCTAACTATTCTCCAACTAGTCCCAACTATACCCCGACATCACCCAGCTACAGC CCAACCTCACCCAGTTACTCACCTACAAGTCCCAACTATACACCCACCAGCCCTAATTACAGCCCAACC TCTCCAAGCTATTCCCCAACCTCACCCAGTTATTCCCCCACCTCACCAAGCTACTCCCCCTCCAGCCCA CGATATACACCACAGTCTCCAACCTACACACCAAGCTCACCAAGCTACAGTCCCAGCTCACCAAGCTAC AGCCCCACTTCACCCAAGTACACCCCAACTAGTCCTTCCTACAGTCCCAGCTCACCAGAGTACACCCCA GCTTCTCCCAAATACTCACCTACAAGCCCTAAATATTCACCCACTTCTCCCAAGTATTCTCCTACCAGC CCCACTTACTCACCTACCACCCCAAAATATTCTCCAACCTCCCCGACATACTCACCAACCTCTCCAGTC TATACCCCGACCTCTCCCAAGTACTCCCCAACCAGCCCTACCTACTCGCCCACTTCTCCCAAGTACTCG CCCACCAGTCCCACCTACTCACCCACCTCTCCCAAGGGCTCCACCTACTCTCCCACTTCTCCTGGCTAC TCACCCACTAGCCCCACCTACAGCCTCACCAGCCCAGCCATCAGCCCAGATGACAGCGATGAGGAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by MR225735
Blue=ORF Red=Cloning site Green=Tag(s) MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTG RCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPK KRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNE DSQEKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDL THKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKG KEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYP GAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFR LNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRD VFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHI SPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIG DSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKS LSEYNNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYL AGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLA GESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVL RVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQE NATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTL NTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANT AIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDD LNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHL PQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALER ELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESD PMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGRSGMTPGAAGFSPSAASDASG FSPGYSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPT SPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSP TSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPNYTPTSPSYS PTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSY SPTSPKYTPTSPSYSPSSPEYTPASPKYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPV YTPTSPKYSPTSPTYSPTSPKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPDDSDEEN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009089 |
| ORF Size | 5796 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009089.2, NP_033115.1 |
| RefSeq Size | 5799 bp |
| RefSeq ORF | 5799 bp |
| Locus ID | 20020 |
| Cytogenetics | 11 42.86 cM |
| MW | 213.5 kDa |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates (By similarity). Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing (By similarity). Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (PubMed:26687004). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression (PubMed:26687004).[UniProtKB/Swiss-Prot Function] |
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