Polr2a (NM_009089) Mouse Tagged ORF Clone

CAT#: MG225735

Polr2a (tGFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide A (Polr2a), (10ug). Note: ORF is codon optimized

Product Images

Circular map for MG225735

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Polr2a
• Synonyms: 220kDa; Rpb1; Rpo2-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225735 representing NM_009089, codon optimized.
  Due to the complexity of NM_009089, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACGGGGGTGGCCCCCCCTCCGGGGACAGCGCATGCCCGCTGCGCACCATCAAGAGAGTGCAGTTCG
  GAGTCCTGAGTCCGGATGAATTGAAGCGGATGTCTGTGACAGAGGGTGGTATCAAATACCCAGAAACAAC
  GGAGGGAGGTCGCCCCAAACTTGGGGGACTAATGGATCCACGGCAGGGGGTGATTGAGCGGACTGGCCGC
  TGCCAAACATGTGCAGGAAACATGACCGAGTGTCCTGGCCACTTTGGCCACATCGAATTGGCCAAACCTG
  TGTTTCATGTGGGCTTCCTGGTGAAGACAATGAAGGTTTTGCGCTGTGTCTGCTTCTTCTGCTCCAAACT
  GCTTGTAGATTCTAATAACCCGAAGATTAAGGACATCCTGGCCAAATCTAAGGGGCAGCCAAAGAAACGG
  CTTACGCATGTCTATGACCTGTGCAAGGGCAAAAACATCTGTGAAGGTGGAGAGGAGATGGACAACAAGT
  TTGGTGTGGAGCAGCCGGAGGGTGATGAGGATCTGACCAAAGAGAAGGGCCATGGCGGCTGTGGGCGGTA
  CCAACCCCGAATCCGGCGCTCTGGCCTAGAGTTGTATGCTGAATGGAAGCATGTTAATGAGGACTCTCAG
  GAGAAAAAGATCTTGCTGAGTCCAGAACGAGTGCATGAAATCTTCAAACGCATCTCAGATGAGGAGTGTT
  TTGTGTTGGGCATGGAGCCTCGCTATGCCCGGCCTGAGTGGATGATTGTCACAGTGTTGCCTGTGCCTCC
  TCTCTCAGTGAGACCTGCTGTGGTGATGCAGGGTTCTGCTCGAAACCAGGATGATCTGACTCACAAACTG
  GCTGACATTGTGAAGATCAACAATCAGCTGCGGCGGAACGAGCAGAATGGTGCAGCTGCCCATGTCATAG
  CAGAAGATGTAAAACTTCTCCAGTTCCATGTGGCCACCATGGTGGACAATGAACTGCCCGGCTTACCCCG
  TGCCATGCAGAAGTCTGGCCGTCCCCTCAAGTCCCTGAAGCAACGGTTGAAGGGCAAAGAAGGACGGGTT
  CGAGGGAATCTAATGGGCAAGCGAGTGGACTTCTCAGCCCGCACTGTCATCACCCCTGACCCTAACCTAT
  CCATTGACCAAGTGGGTGTGCCCCGCTCCATTGCTGCCAACATGACCTTTGCAGAGATTGTCACACCCTT
  CAATATTGACAGACTTCAGGAATTAGTACGCAGGGGAAACAGCCAGTATCCAGGAGCCAAGTACATCATC
  CGCGATAATGGCGATCGAATTGACTTGCGTTTCCATCCTAAGCCCAGTGACCTTCATCTGCAGACTGGCT
  ATAAGGTGGAACGGCACATGTGTGATGGGGACATTGTTATCTTCAATCGTCAGCCAACTTTGCACAAAAT
  GTCCATGATGGGGCATCGGGTCCGAATCCTCCCCTGGTCTACTTTTCGGTTGAATCTTAGTGTGACAACT
  CCATACAATGCAGACTTTGATGGAGATGAGATGAATTTGCATCTGCCACAGTCTCTGGAAACACGGGCAG
  AGATCCAAGAGTTGGCTATGGTGCCTCGAATGATTGTCACCCCCCAGAGCAATCGACCTGTCATGGGTAT
  TGTGCAAGACACACTCACGGCAGTACGCAAATTCACTAAGAGAGATGTCTTCCTGGAGCGGGGTGAAGTC
  ATGAACCTCTTAATGTTTCTGTCCACGTGGGATGGCAAGGTCCCACAACCAGCTATCCTCAAGCCCCGGC
  CCCTGTGGACAGGCAAGCAGATCTTCTCCCTCATCATACCTGGACACATCAATTGTATCCGTACCCACAG
  CACCCATCCTGATGATGAAGATAGTGGCCCTTACAAGCACATCTCTCCTGGGGACACCAAAGTTGTAGTG
  GAGAATGGGGAGCTTATCATGGGCATCCTTTGTAAGAAGTCCCTGGGCACCTCAGCAGGCTCGCTGGTCC
  ATATCTCCTACCTGGAGATGGGGCATGACATCACCCGCCTCTTCTACTCCAACATTCAGACTGTCATTAA
  CAACTGGCTTCTCATCGAGGGTCATACCATTGGCATTGGGGACTCCATTGCAGATTCAAAGACTTACCAG
  GACATTCAGAACACTATTAAGAAAGCTAAACAAGATGTAATAGAGGTCATTGAGAAGGCTCATAACAATG
  AGCTAGAACCCACTCCAGGAAACACATTGCGTCAGACATTTGAGAATCAAGTGAATCGTATTCTCAATGA
  TGCTCGAGACAAAACTGGCTCCTCTGCACAGAAATCCCTCTCTGAATATAACAACTTCAAGTCTATGGTG
  GTGTCTGGAGCCAAGGGTTCCAAGATCAACATCTCCCAGGTCATTGCTGTTGTCGGGCAGCAGAATGTAG
  AGGGCAAGCGGATCCCATTTGGATTCAAGCATCGGACTCTTCCTCACTTTATCAAGGATGATTATGGTCC
  TGAGAGCCGAGGCTTTGTAGAAAATTCATACTTGGCTGGTCTCACACCCACTGAGTTCTTCTTCCATGCC
  ATGGGAGGACGAGAAGGTCTCATTGACACGGCTGTTAAGACTGCTGAGACTGGGTATATTCAGCGAAGGC
  TGATAAAGTCCATGGAGTCGGTGATGGTGAAATATGATGCAACTGTGAGGAACTCCATCAACCAGGTGGT
  ACAGCTGCGCTATGGAGAGGACGGCCTGGCAGGGGAGAGTGTTGAGTTCCAGAATCTGGCTACACTTAAA
  CCTTCTAATAAAGCTTTTGAGAAGAAGTTCCGTTTTGATTATACCAATGAGAGGGCCCTGCGGCGCACCC
  TTCAGGAGGACCTGGTAAAGGATGTGCTGAGCAATGCCCATATACAGAATGAGCTGGAACGGGAATTTGA
  ACGGATGCGTGAAGATCGGGAAGTGCTCAGGGTCATCTTCCCAACTGGTGACAGCAAGGTGGTCCTCCCT
  TGTAACCTTCTGCGGATGATCTGGAATGCTCAGAAAATCTTCCACATCAACCCTCGCCTTCCCTCTGACC
  TGCATCCCATCAAAGTGGTAGAGGGAGTCAAAGAATTGAGCAAGAAGTTGGTAATTGTGAATGGAGATGA
  CCCACTAAGTCGGCAGGCACAGGAGAATGCCACATTGCTCTTCAACATCCACCTAAGATCCACACTCTGC
  TCTCGCCGCATGGCTGAGGAGTTTCGGCTGAGTGGAGAGGCCTTTGACTGGCTGCTTGGGGAGATAGAGT
  CCAAGTTCAACCAAGCCATTGCCCATCCTGGAGAAATGGTGGGAGCTCTGGCTGCTCAGTCTCTTGGAGA
  ACCTGCCACTCAGATGACCCTGAACACTTTCCACTATGCTGGTGTATCTGCCAAGAATGTGACGCTGGGT
  GTGCCTCGACTTAAGGAGCTTATCAACATTTCCAAGAAGCCAAAGACTCCTTCACTCACTGTCTTCCTGT
  TAGGTCAATCTGCACGAGATGCTGAAAGAGCCAAGGACATTCTTTGCCGCTTGGAACATACAACATTGAG
  GAAGGTGACTGCCAATACAGCCATCTACTATGACCCTAACCCCCAGAGCACAGTGGTGGCAGAAGATCAG
  GAGTGGGTGAATGTGTACTATGAGATGCCCGACTTTGACGTGGCCCGGATATCTCCCTGGCTGCTGCGTG
  TGGAGCTCGACCGAAAGCACATGACTGACCGGAAGCTTACCATGGAACAGATTGCTGAGAAAATCAATGC
  AGGTTTTGGCGATGATTTGAATTGTATCTTCAACGATGATAATGCAGAGAAGCTGGTCCTTCGAATCCGC
  ATCATGAACAGTGATGAAAACAAGATGCAAGAGGAGGAAGAGGTGGTGGATAAAATGGATGATGATGTCT
  TCCTGCGATGCATTGAGTCCAACATGCTGACAGATATGACCCTGCAGGGTATCGAGCAGATCAGCAAGGT
  GTACATGCACTTACCTCAGACAGACAACAAGAAGAAGATCATCATCACAGAGGATGGGGAGTTCAAGGCC
  CTGCAGGAATGGATCTTAGAGACGGATGGTGTGAGCCTGATGCGGGTGCTGAGTGAGAAGGATGTAGACC
  CTGTGCGCACCACGTCCAATGATATTGTGGAGATCTTCACGGTACTGGGCATTGAGGCTGTGCGGAAGGC
  TCTGGAGCGAGAACTGTACCATGTCATCTCCTTTGATGGTTCTTATGTCAATTATCGGCATTTGGCGCTC
  CTGTGTGACACTATGACATGCCGTGGCCACTTGATGGCCATCACTCGTCATGGGGTCAACCGCCAGGACA
  CTGGACCGCTCATGAAATGCTCCTTTGAGGAAACGGTGGATGTCCTTATGGAAGCAGCAGCACATGGGGA
  GAGTGACCCGATGAAGGGAGTCTCTGAGAATATTATGCTGGGCCAGCTCGCTCCAGCTGGTACTGGCTGT
  TTTGACCTCCTGCTTGATGCTGAGAAGTGCAAATACGGCATGGAAATCCCCACCAATATCCCTGGCCTGG
  GGGCTGCTGGACGGAGCGGGATGACCCCAGGAGCGGCCGGCTTCTCTCCCAGTGCTGCATCTGATGCCAG
  TGGCTTCAGCCCAGGTTACTCCCCTGCATGGTCTCCCACACCAGGCTCTCCGGGCTCCCCTGGACCCTCA
  AGCCCATACATCCCCTCACCAGGTGGTGCTATGTCTCCCAGCTACTCACCGACATCACCAGCCTATGAGC
  CACGCTCCCCTGGGGGCTATACACCCCAGAGCCCCTCCTACTCCCCTACTTCACCTTCCTACTCCCCAAC
  CTCTCCATCTTACTCTCCAACCAGTCCCAACTACAGCCCTACCTCTCCTAGCTACTCGCCCACCTCCCCT
  AGCTACTCGCCAACCTCTCCTTCCTACTCCCCCACCTCTCCAAGCTATTCCCCAACCTCTCCTAGCTACT
  CCCCAACCTCTCCAAGCTATTCTCCAACATCACCTAGCTATTCTCCAACTTCTCCCAGCTACTCACCGAC
  ATCTCCTAGCTACTCCCCAACTTCTCCCAGCTACTCACCAACTTCACCAAGCTATTCTCCCACCTCCCCC
  AGCTACTCACCGACATCTCCCAGCTACTCACCAACTTCTCCAAGCTACTCACCAACTTCTCCAAGCTACT
  CACCCACCAGCCCTAACTATTCTCCAACTAGTCCCAACTATACCCCGACATCACCCAGCTACAGCCCAAC
  CTCACCCAGTTACTCACCTACAAGTCCCAACTATACACCCACCAGCCCTAATTACAGCCCAACCTCTCCA
  AGCTATTCCCCAACCTCACCCAGTTATTCCCCCACCTCACCAAGCTACTCCCCCTCCAGCCCACGATATA
  CACCACAGTCTCCAACCTACACACCAAGCTCACCAAGCTACAGTCCCAGCTCACCAAGCTACAGCCCCAC
  TTCACCCAAGTACACCCCAACTAGTCCTTCCTACAGTCCCAGCTCACCAGAGTACACCCCAGCTTCTCCC
  AAATACTCACCTACAAGCCCTAAATATTCACCCACTTCTCCCAAGTATTCTCCTACCAGCCCCACTTACT
  CACCTACCACCCCAAAATATTCTCCAACCTCCCCGACATACTCACCAACCTCTCCAGTCTATACCCCGAC
  CTCTCCCAAGTACTCCCCAACCAGCCCTACCTACTCGCCCACTTCTCCCAAGTACTCGCCCACCAGTCCC
  ACCTACTCACCCACCTCTCCCAAGGGCTCCACCTACTCTCCCACTTCTCCTGGCTACTCACCCACTAGCC
  CCACCTACAGCCTCACCAGCCCAGCCATCAGCCCAGATGACAGCGATGAGGAGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225735 representing NM_009089
  Red=Cloning site Green=Tags(s)
  
  MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGR
  CQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKR
  LTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQ
  EKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKL
  ADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRV
  RGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYII
  RDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTT
  PYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEV
  MNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVV
  ENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQ
  DIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMV
  VSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHA
  MGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLK
  PSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLP
  CNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLC
  SRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLG
  VPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQ
  EWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIR
  IMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKA
  LQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLAL
  LCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGC
  FDLLLDAEKCKYGMEIPTNIPGLGAAGRSGMTPGAAGFSPSAASDASGFSPGYSPAWSPTPGSPGSPGPS
  SPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSP
  SYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP
  SYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSP
  SYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPTSPKYTPTSPSYSPSSPEYTPASP
  KYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTSPKYSPTSP
  TYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPDDSDEEN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009089
• ORF Size: 5796 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009089.2, NP_033115.1
• RefSeq Size: 5799 bp
• RefSeq ORF: 5799 bp
• Locus ID: 20020
• Cytogenetics: 11 42.86 cM
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates (By similarity). Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing (By similarity). Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (PubMed:26687004). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression (PubMed:26687004).[UniProtKB/Swiss-Prot Function]