Cldn22 (NM_029383) Mouse Tagged ORF Clone

CAT#: MR225640

Cldn22 (Myc-DDK-tagged) - Mouse claudin 22 (Cldn22)

Product Images

Circular map for MR225640

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cldn22
• Synonyms: 2210404A22Rik; 5330431C14Rik; 9530051B05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225640 representing NM_029383
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTTAGTCTTCCGAACGGCAACGCAGGCGGCTGCACTTCTGCTCTCCCTGCTGGGATGGGTTTTAT
  CCTGCCTTACAAACTACTTGCCGCACTGGAAGAACCTTAACTTGGAGCTGAACGAGATGGAGAACTGGAC
  CATGGGGCTCTGGAAATCCTGCGTCATCCAGGAGGAAGTCGGGAGGCAATGCAAGGACTTTGACTCCTTC
  CTGGCCTTGCCAGCTGAACTGCAGGTCTCCAGGGTCTTGATGTCCCTGTGCAACGGGCTGGGGCTGCTGG
  GCTTGCTGGCCTCTGGCTGTGGCCTGGACTGCTTGAGGCTTGGAGAGACACAGGAGGGTCTGAAGAAGCG
  GCTGCTCACCCTGGGAGGGACCCTGCTCTGGACCTCGGGAGTCATGGTGCTAGTTCCTGTCTCCTGGGTG
  GCCCACAAGACGGTGCGGGAGTTCTGGGATGAGACCATGCCTGAGATTGTGCCCAGGTGGGAGTTTGGGG
  AGGCCCTGTTCCTGGGCTGGTTTGCTGGCTTCTGCCTGGTGCTAGGAGGGTGTGTGCTTCACTGTGCAGC
  CTGCTGGAGCCCGGCTCCTGCAGCCTCGAGTCACTATGCAGTGGCGGGACCTCGAGACCACCAGCAGCAT
  CTGGAGCTGAAGCAAGCCAATCCAGAAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225640 representing NM_029383
  Red=Cloning site Green=Tags(s)
  
  MGLVFRTATQAAALLLSLLGWVLSCLTNYLPHWKNLNLELNEMENWTMGLWKSCVIQEEVGRQCKDFDSF
  LALPAELQVSRVLMSLCNGLGLLGLLASGCGLDCLRLGETQEGLKKRLLTLGGTLLWTSGVMVLVPVSWV
  AHKTVREFWDETMPEIVPRWEFGEALFLGWFAGFCLVLGGCVLHCAACWSPAPAASSHYAVAGPRDHQQH
  LELKQANPEI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029383
• ORF Size: 660 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029383.1, NP_083659.1
• RefSeq Size: 994 bp
• RefSeq ORF: 663 bp
• Locus ID: 75677
• UniProt ID: Q9D7U6
• Cytogenetics: 8 B1.1
• MW: 24.8 kDa
• Gene Summary: This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is intronless and overlaps the 3' UTR of the Wwc2 gene (GeneID: 52357) on the opposite strand. [provided by RefSeq, Aug 2010]