P4htm (NM_028944) Mouse Tagged ORF Clone

CAT#: MR225578

P4htm (Myc-DDK-tagged) - Mouse prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4htm)

Product Images

Circular map for MR225578

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: P4htm
• Synonyms: 4933406E20Rik; AI853847; BB128974; Ph-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225578 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGCGGTGGCGACCGTGCAGCGGCCTGAGGCTGAGACAGTCGAGGAGGCCTCAAATCTTCAGT
  GGCCGCTACCACCGGAGCATCGCCCTTCCGGGGCAGCGACCAGACCAGGCGATAGTGAAGACGCCCCGGT
  GCGGCCACTGTGCAAGCCGCGGGGCATCTGTTCCCGTGCCTACTTCTTGGTGCTGATGGTGTTCGTGCAC
  CTGTACTTGGGCAACGTGCTGGCCCTGCTGCTCTTCGTGCACTACAGCAACGGAGACGAGAGCACAGACC
  CCGGACCCCAGCGCCGCGAGCAGAGCCCCCAGCCAGTGCCAACCTTGGGTCCCCTCACCCGGCTGGAGGG
  CATCAAGGTGGGATATGAGCGGAAAGTCCAGGTGGTCGCGGGCAGGGATCACTTCATCCGAACCCTCAGC
  CTCAAGCCGCTGCTTTTTGAAATCCCAGGCTTCCTGAGTGACGAGGAGTGTCGGCTCATCATCCACCTGG
  CACAGATGAAAGGGTTACAGCGAAGCCAGATCCTGCCCACTGAGGAGTATGAGGAGGCGATGAGCGCCAT
  GCAGGTCAGCCAGCTGGACCTCTTCCAGCTGTTGGATCAGAACCACGACGGTCGCCTGCAGCTCCGAGAG
  GTCCTGGCCCAGACTCGCCTGGGAAATGGACGGTGGATGACTCCAGAGAACATTCAGGAGATGTACTCTG
  CAATCAAAGCAGACCCTGACGGTGATGGCGTGCTGAGTCTGCAGGAATTTTCCAACATGGACCTCCGAGA
  CTTCCACAAGTACATGAGGAGCCATAAGGCAGAATCCAATGAGCTGGTGAGGAACAGCCACCACACATGG
  CTCCACCAGGGAGAGGGAGCACATCACGTCATGCGTGCCATCCGCCAGAGGGTCCTGCGCCTCACTCGCC
  TCTCCCCGGAGATTGTGGAGTTCAGTGAGCCACTACAGGTTGTACGATATGGTGAGGGAGGTCACTACCA
  TGCCCATGTGGACAGCGGACCCGTGTACCCAGAGACCATCTGCTCCCATACCAAGCTGGTAGCCAATGAA
  TCTGTACCGTTTGAGACCTCCTGTCGCTACATGACAGTGCTGTTTTATTTGAACAATGTCACCGGTGGGG
  GTGAGACCGTCTTCCCTGTGGCAGACAACAGGACCTATGATGAAATGAGTCTGATTCAGGATGATGTCGA
  TCTCCGGGACACTCGAAGGCACTGTGACAAGGGCAACCTTCGTGTCAAGCCCCAGCAAGGAACTGCAGTC
  TTTTGGTACAACTACCTTCCTGATGGGCAAGGCTGGGTGGGTGAAGTGGACGACTACTCGTTGCATGGGG
  GCTGCCTGGTCACCCGCGGCACCAAGTGGATCGCTAACAACTGGATCAATGTGGACCCTAGCCGGGCACG
  GCAAGCCTTGTTCCAGCAGGAGATGGCTCGTCTGGCTCGGGAAGGTGGCATGGACTCACAACCCGAGTGG
  GCCTTGGACCGAGCCTACAGTGATGCCCGTGTGGAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225578 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAVATVQRPEAETVEEASNLQWPLPPEHRPSGAATRPGDSEDAPVRPLCKPRGICSRAYFLVLMVFVH
  LYLGNVLALLLFVHYSNGDESTDPGPQRREQSPQPVPTLGPLTRLEGIKVGYERKVQVVAGRDHFIRTLS
  LKPLLFEIPGFLSDEECRLIIHLAQMKGLQRSQILPTEEYEEAMSAMQVSQLDLFQLLDQNHDGRLQLRE
  VLAQTRLGNGRWMTPENIQEMYSAIKADPDGDGVLSLQEFSNMDLRDFHKYMRSHKAESNELVRNSHHTW
  LHQGEGAHHVMRAIRQRVLRLTRLSPEIVEFSEPLQVVRYGEGGHYHAHVDSGPVYPETICSHTKLVANE
  SVPFETSCRYMTVLFYLNNVTGGGETVFPVADNRTYDEMSLIQDDVDLRDTRRHCDKGNLRVKPQQGTAV
  FWYNYLPDGQGWVGEVDDYSLHGGCLVTRGTKWIANNWINVDPSRARQALFQQEMARLAREGGMDSQPEW
  ALDRAYSDARVEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028944
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028944.4
• RefSeq Size: 1810 bp
• RefSeq ORF: 1512 bp
• Locus ID: 74443
• UniProt ID: Q8BG58
• Cytogenetics: 9 F2
• MW: 57 kDa
• Gene Summary: Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.[UniProtKB/Swiss-Prot Function]