P4htm (NM_028944) Mouse Untagged Clone

CAT#: MC217025

P4htm (untagged) - Mouse prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) (P4htm), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: P4htm
• Synonyms: 4933406E20Rik; AI853847; BB128974; Ph-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217025 representing NM_028944
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGCGGTGGCGACCGTGCAGCGGCCTGAGGCTGAGACAGTCGAGGAGGCCTCAAATCTTCAGT
  GGCCGCTACCACCGGAGCATCGCCCTTCCGGGGCAGCGACCAGACCAGGCGATAGTGAAGACGCCCCGGT
  GCGGCCACTGTGCAAGCCGCGGGGCATCTGTTCCCGTGCCTACTTCTTGGTGCTGATGGTGTTCGTGCAC
  CTGTACTTGGGCAACGTGCTGGCCCTGCTGCTCTTCGTGCACTACAGCAACGGAGACGAGAGCACAGACC
  CCGGACCCCAGCGCCGCGAGCAGAGCCCCCAGCCAGTGCCAACCTTGGGTCCCCTCACCCGGCTGGAGGG
  CATCAAGGTGGGATATGAGCGGAAAGTCCAGGTGGTCGCGGGCAGGGATCACTTCATCCGAACCCTCAGC
  CTCAAGCCGCTGCTTTTTGAAATCCCAGGCTTCCTGAGTGACGAGGAGTGTCGGCTCATCATCCACCTGG
  CACAGATGAAAGGGTTACAGCGAAGCCAGATCCTGCCCACTGAGGAGTATGAGGAGGCGATGAGCGCCAT
  GCAGGTCAGCCAGCTGGACCTCTTCCAGCTGTTGGATCAGAACCACGACGGTCGCCTGCAGCTCCGAGAG
  GTCCTGGCCCAGACTCGCCTGGGAAATGGACGGTGGATGACTCCAGAGAACATTCAGGAGATGTACTCTG
  CAATCAAAGCAGACCCTGACGGTGATGGCGTGCTGAGTCTGCAGGAATTTTCCAACATGGACCTCCGAGA
  CTTCCACAAGTACATGAGGAGCCATAAGGCAGAATCCAATGAGCTGGTGAGGAACAGCCACCACACATGG
  CTCCACCAGGGAGAGGGAGCACATCACGTCATGCGTGCCATCCGCCAGAGGGTCCTGCGCCTCACTCGCC
  TCTCCCCGGAGATTGTGGAGTTCAGTGAGCCACTACAGGTTGTACGATATGGTGAGGGAGGTCACTACCA
  TGCCCATGTGGACAGCGGACCCGTGTACCCAGAGACCATCTGCTCCCATACCAAGCTGGTAGCCAATGAA
  TCTGTACCGTTTGAGACCTCCTGTCGCTACATGACAGTGCTGTTTTATTTGAACAATGTCACCGGTGGGG
  GTGAGACCGTCTTCCCTGTGGCAGACAACAGGACCTATGATGAAATGAGTCTGATTCAGGATGATGTCGA
  TCTCCGGGACACTCGAAGGCACTGTGACAAGGGCAACCTTCGTGTCAAGCCCCAGCAAGGAACTGCAGTC
  TTTTGGTACAACTACCTTCCTGATGGGCAAGGCTGGGTGGGTGAAGTGGACGACTACTCGTTGCATGGGG
  GCTGCCTGGTCACCCGCGGCACCAAGTGGATCGCTAACAACTGGATCAATGTGGACCCTAGCCGGGCACG
  GCAAGCCTTGTTCCAGCAGGAGATGGCTCGTCTGGCTCGGGAAGGTGGCATGGACTCACAACCCGAGTGG
  GCCTTGGACCGAGCCTACAGTGATGCCCGTGTGGAGCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028944
• Insert Size: 1512 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028944.3, NP_083220.3
• RefSeq Size: 1810 bp
• RefSeq ORF: 1512 bp
• Locus ID: 74443
• UniProt ID: Q8BG58
• Cytogenetics: 9 F2
• Gene Summary: Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.