Pde4d (NM_011056) Mouse Tagged ORF Clone

CAT#: MR225474

Pde4d (Myc-DDK-tagged) - Mouse phosphodiesterase 4D, cAMP specific (Pde4d)

Product Images

Circular map for MR225474

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pde4d
• Synonyms: 9630011N22Rik; Dpde3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225474 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGGGACACTTGTGATGTACTCTCTCGGAGCAAAAGTGCCTCTGAGGAAACACTGCACTCCTGTA
  ATGAAGAAGAGGACCCTTTTCGTGGAATGGAGCCCTATCTCGTACGGCGACTTTCTTCCCGCAGCATTCA
  GCTTCCACCTCTGGCCTTCAGACAGTTGGAACAAGCAGACTTGCGAAGCGAATCAGAGAACATCCCTCGG
  CCAACTAGCCTCCCCTTGAAAATCCTGCCTCTGATCGCCGTCACATCTGCAGACTCAAGTGGTTTCGATG
  TGGACAATGGCACATCAGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCAGGGTCTGGGCTGATTCT
  CCAAGCAAACTTTGTCCACAGTCAACGCCGGGAGTCCTTCCTGTACCGATCTGACAGCGACTATGACCTC
  TCTCCAAAGTCTATGTCCAGGAACTCCTCGATTGCCAGTGATATACATGGAGATGACTTGATTGTGACCC
  CGTTTGCTCAGGTCTTGGCTAGCCTGCGAACCGTACGGAACAACTTTGCTGCGTTAACGAATTTGCAAGA
  CCGAGCACCCAGCAAAAGATCACCCATGTGCAACCAACCATCCATCAACAAAGCCACCATCACAGAGGAG
  GCCTACCAGAAACTGGCCAGCGAGACCCTGGAGGAGCTGGACTGGTGTCTGGACCAGCTAGAGACCCTGC
  AGACCAGGCACTCCGTCAGTGAGATGGCCTCCAACAAGTTCAAAAGGATGCTGAACCGGGAGCTCACGCA
  TCTCTCTGAGATGAGTCGGTCTGGCAACCAGGTGTCGGAGTACATCTCAAACACATTTCTCGATAAGCAA
  CATGAAGTGGAAATCCCCTCTCCAACTCAGAAGGAAAAGGAGAAGAAGAAAAGGCCGATGTCGCAGATCA
  GTGGGGTCAAGAAGTTGATGCACAGCTCCAGCCTAACTAATTCATGTATCCCCAGGTTTGGGGTTAAAAC
  GGAGCAGGAAGATGTCCTGGCCAAGGAACTAGAAGACGTGAACAAGTGGGGCCTCCACGTTTTCCGAATA
  GCAGAGCTGTCTGGTAACCGGCCTCTGACTGTTATCATGCACACCATTTTTCAGGAACGAGATTTGTTAA
  AAACGTTTAAAATCCCAGTAGACACTTTGATTACGTATCTTATGACTCTGGAAGACCATTACCATGCTGA
  CGTGGCCTATCACAACAACATCCATGCTGCAGACGTCGTCCAGTCCACTCACGTGCTGCTCTCTACACCC
  GCTTTGGAGGCTGTGTTCACAGACTTGGAGATTCTCGCGGCCATTTTTGCCAGTGCAATACATGATGTGG
  ACCATCCTGGGGTGTCAAATCAATTTCTGATCAATACAAACTCGGAACTCGCTCTGATGTACAACGACTC
  CTCGGTCCTAGAGAACCATCACCTGGCGGTGGGCTTTAAGTTGCTCCAGGAAGAAAACTGTGACATTTTC
  CAGAATCTGACCAAAAAGCAAAGACAATCTTTAAGGAAAATGGTCATTGACATTGTCCTGGCGACAGACA
  TGTCGAAGCACATGAACCTGCTGGCTGATCTGAAAACCATGGTTGAAACCAAGAAGGTGACAAGCTCCGG
  CGTCCTCCTCCTGGATAACTACTCCGACAGGATCCAGGTCCTTCAGAATATGGTGCACTGTGCAGACCTG
  AGCAACCCCACAAAGCCACTCCAGCTGTACCGCCAGTGGACGGACCGGATAATGGAGGAGTTCTTCCGCC
  AGGGGGACCGAGAGCGGGAGCGTGGCATGGAGATAAGTCCCATGTGTGACAAGCACAACGCCTCTGTGGA
  AAAATCACAGGTGGGCTTCATAGACTATATCGTTCATCCACTCTGGGAGACGTGGGCAGACCTCGTACAT
  CCCGATGCCCAGGACATCTTGGACACTTTGGAGGACAATCGTGAGTGGTACCAGAGCACAATCCCCCAGA
  GCCCCTCCCCTGCACCTGATGACCAAGAGGAGGGCCGGCAGGGACAGACTGAAAAATTCCAGTTTGAACT
  AACCTTGGAGGAAGACTGTGAGTCAGACACTGAAAAGGACAGTGGAAGTCAGGTGGAGGAAGACACTAGC
  TGCAGTGACTCTAAGACTCTGTGCACCCAAGACTCAGAGTCCACTGAAATTCCCCTTGACGAGCAGGTTG
  AGGAGGAAGCTGTAGCAGAAGAGGAAAGCCAGCCTGAAACTTGCGTCCCAGATGACTGCTGTCCTGATAC
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225474 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MERDTCDVLSRSKSASEETLHSCNEEEDPFRGMEPYLVRRLSSRSIQLPPLAFRQLEQADLRSESENIPR
  PTSLPLKILPLIAVTSADSSGFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDL
  SPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEE
  AYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQ
  HEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSCIPRFGVKTEQEDVLAKELEDVNKWGLHVFRI
  AELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTP
  ALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIF
  QNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADL
  SNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVH
  PDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEEGRQGQTEKFQFELTLEEDCESDTEKDSGSQVEEDTS
  CSDSKTLCTQDSESTEIPLDEQVEEEAVAEEESQPETCVPDDCCPDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011056
• ORF Size: 2244 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011056.1, NM_011056.2, NM_011056.3, NP_035186.1
• RefSeq Size: 7049 bp
• RefSeq ORF: 2244 bp
• Locus ID: 238871
• UniProt ID: Q01063
• Cytogenetics: 13 59.69 cM
• MW: 84.6 kDa
• Gene Summary: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.[UniProtKB/Swiss-Prot Function]