Spib (NM_019866) Mouse Tagged ORF Clone

CAT#: MR225440

Spib (Myc-DDK-tagged) - Mouse Spi-B transcription factor (Spi-1/PU.1 related) (Spib)

Product Images

Circular map for MR225440

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Spib
• Synonyms: Spi-B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225440 representing NM_019866
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTGCTCTGGAGGCTGCACAGCTGGATGGCCCACACTTAAGCTGTTTGTACCCAGAAGGAGTCTTCT
  ACGACCTGGACAGCTGCAAGCCCTTCAGTTACCCAGATTCAGATGGGGGCCTTGACTCTACATGGGGCTG
  GACAGAGGCCCCGCCTGCTCCTGCCATCGCTCCCTATGAAGCCTTCGATCCTGCTACTGCTGCCTTTAGC
  CACTCCCAAACTGTTCAGCTCTGTTATAGCCATGGTCCTAACCCCTCCACCTATAGCCCCATGGGGACCC
  TCGACCCAGCCCCCAGCTTGGAGGCCCCAGGGCCTGGCCTCCAGGTGTACCCCCCAGAGGACTTCACCAG
  CCAGACCCTGGGCTCCTTGGCTTATGCTCCGTACCCCAGCCCTGTGCTATCAGAGGAAGAAGACATTATG
  CTGGACAGCCCCGCCCTGGAGGTCTCGGACAGTGAGTCAGACGAGGCCCTCTTGGCTGGCTCCGAGGGGA
  GGGGATCTGAGGCAGGTGCACGCAAGAAGCTGCGCCTGTACCAGTTCTTGCTGGGGTTGCTCCTACGCGG
  GGACATGCGCGAGTGCGTGTGGTGGGTGGAGCCAGGTGCCGGCGTCTTCCAGTTCTCCTCCAAGCACAAG
  GAGTTGTTGGCTCGCCGCTGGGGCCAGCAGAAGGGCAACCGCAAGCGCATGACGTATCAGAAGCTGGCCC
  GAGCGCTGCGCAACTATGCCAAGACAGGCGAAATCCGCAAGGTCAAACGCAAACTCACCTACCAGTTTGA
  CAGCGCGCTGCTGCCAGCCTCCCGGCATGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225440 representing NM_019866
  Red=Cloning site Green=Tags(s)
  
  MLALEAAQLDGPHLSCLYPEGVFYDLDSCKPFSYPDSDGGLDSTWGWTEAPPAPAIAPYEAFDPATAAFS
  HSQTVQLCYSHGPNPSTYSPMGTLDPAPSLEAPGPGLQVYPPEDFTSQTLGSLAYAPYPSPVLSEEEDIM
  LDSPALEVSDSESDEALLAGSEGRGSEAGARKKLRLYQFLLGLLLRGDMRECVWWVEPGAGVFQFSSKHK
  ELLARRWGQQKGNRKRMTYQKLARALRNYAKTGEIRKVKRKLTYQFDSALLPASRHV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019866
• ORF Size: 801 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019866.1, NP_063919.1
• RefSeq Size: 2786 bp
• RefSeq ORF: 804 bp
• Locus ID: 272382
• UniProt ID: O35906
• Cytogenetics: 7 28.83 cM
• MW: 29.8 kDa
• Gene Summary: Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. Required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.[UniProtKB/Swiss-Prot Function]