Spib (NM_019866) Mouse Untagged Clone

CAT#: MC214320

Spib (untagged) - Mouse Spi-B transcription factor (Spi-1/PU.1 related) (Spib), (10ug)


  "NM_019866" in other vectors (4)

Reconstitution Protocol

USD 480.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Spib
Synonyms Spi-B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC214320 representing NM_019866
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTGCTCTGGAGGCTGCACAGCTGGATGGCCCACACTTAAGCTGTTTGTACCCAGAAGGAGTCTTCT
ACGACCTGGACAGCTGCAAGCCCTTCAGTTACCCAGATTCAGATGGGGGCCTTGACTCTACATGGGGCTG
GACAGAGGCCCCGCCTGCTCCTGCCATCGCTCCCTATGAAGCCTTCGATCCTGCTACTGCTGCCTTTAGC
CACTCCCAAACTGTTCAGCTCTGTTATAGCCATGGTCCTAACCCCTCCACCTATAGCCCCATGGGGACCC
TCGACCCAGCCCCCAGCTTGGAGGCCCCAGGGCCTGGCCTCCAGGTGTACCCCCCAGAGGACTTCACCAG
CCAGACCCTGGGCTCCTTGGCTTATGCTCCGTACCCCAGCCCTGTGCTATCAGAGGAAGAAGACATTATG
CTGGACAGCCCCGCCCTGGAGGTCTCGGACAGTGAGTCAGACGAGGCCCTCTTGGCTGGCTCCGAGGGGA
GGGGATCTGAGGCAGGTGCACGCAAGAAGCTGCGCCTGTACCAGTTCTTGCTGGGGTTGCTCCTACGCGG
GGACATGCGCGAGTGCGTGTGGTGGGTGGAGCCAGGTGCCGGCGTCTTCCAGTTCTCCTCCAAGCACAAG
GAGTTGTTGGCTCGCCGCTGGGGCCAGCAGAAGGGCAACCGCAAGCGCATGACGTATCAGAAGCTGGCCC
GAGCGCTGCGCAACTATGCCAAGACAGGCGAAATCCGCAAGGTCAAACGCAAACTCACCTACCAGTTTGA
CAGCGCGCTGCTGCCAGCCTCCCGGCATGTCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_019866
Insert Size 804 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_019866.1, NP_063919.1
RefSeq Size 2786 bp
RefSeq ORF 804 bp
Locus ID 272382
UniProt ID O35906
Cytogenetics 7 28.83 cM
Gene Summary Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. Required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.