Tmprss7 (NM_172455) Mouse Tagged ORF Clone

CAT#: MR225414

Tmprss7 (Myc-DDK-tagged) - Mouse transmembrane serine protease 7 (Tmprss7), transcript variant 2

Product Images

Circular map for MR225414

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tmprss7
• Synonyms: B230219I23Rik; Gm1748
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225414 representing NM_172455
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAAAGAAAAAAGTGATCCTTCCTGCAAGTCCTCAGACCTCAAAATATCCAACATCTCCATCCAGG
  TGGTCAGTGTCCCAGGGAAGCTACCAGGGAGAAGACCACCTCGAAAACCCATTGGCAAACCCAGGCCCCG
  CAAGCAGCCTAAGAAGAGAGCTCCCTTTTGGAATGTTCAAAATAAGATTATTCTCTTCACAGTGTTTTTA
  TTCATTCTGGCAGTCACAGCCTGGACACTTCTATGGCTCTACATCAGTAAGACAGAGAGCAAAGATGCTT
  TTTATTTTGTTGGAATGTTCCGCATCACCAACATTGAGTTCCTTCCTGAGTACCGGCAGAAGGAGTCTAG
  GGAATTTCTCTCCATGGCAAAGACAGTACAGCAAGTGGTAAACCTTGTTTATACAACATCTGCTTTCTCC
  AAATTTTATAAGCAGTCTGTGGTTGCAGACGTCAGCAGTAACAACAAAGGCGGTCTCCTGGTCCACTTTT
  GGATTGTGTTTGTTATGCCACATGCCAAGGGCCACATCTTCTGTGAGGAGTGTGTGGCGGCCATCTTGAA
  GGACTCCATTCAAACCAGTATCATAAACCGCACTTCTGTGGGGAGCTTGCAGGGTCTGGCTGTGGATATG
  GACTCTGTGGTATTAAATGCTGGGCTCCGGTCAGATTACTCTTCAGCTGTAGGATCTGACAACGGCTGCT
  CCCGGTACTTGTATGCAGACCATCTGACCCTCCGCTACCCTCTGGAGATTTCCGCAACCTCGGGACAACT
  AATGTGTCACTTCAAGTTGGTGGCCATCGTGGGTTATCTGATTCGTCTCTCCATTGAATCCATCCAATTG
  GAAGCCGACAACTGTATCACCGACTCCCTGACTGTTTATGACTCCCTCTTGCCAATCCGGAGTGCCATTC
  TATACAGAATCTGTGAACCCACAAGAACACTAATGTCCTTCGTTTCTACAAATAATCTCATGTTGGTGAT
  ACTGAAGTCTCCTTATGTGCGAAGGCTGGCAGGGATCCGAGCATATTTTGAAGTCATTCCAGAACAAAAA
  TGTGAAAGCACAATACTGGTCAAGGAAATCAACAGCTTTGAGGGGAAAATCTCAAGTCCGTATTACCCAA
  GTTACTATCCTCCGAAATGCAAGTGCACCTGGACATTCCAGACTTCCCTATCAACTCTCGGCATAGCCCT
  GAAATTCTACAACTATTCCATAACTAAGAAGAGTGCAAAAGGCTGTGAGCACGGCTGGTGGGAGATTAAT
  GAACACATGTACTGCGGCTCCTACATGGATCATGAGACCATTTTCCGGGTGCCCAGCCCCCTGGTCCACA
  TCCAGCTCCAATGCAGTTCCAGGCTTTCAGACAAGCCACTCTTGGTAGAATATGGCGGTTACAACATCAG
  TCAACCGTGTCCTGCAGGATCTTTTAGGTGCTCCTCCGGTTTGTGTGTCCCTCAGGCCCAGCGTTGTGAC
  GGAGTGAATGACTGTTTCGACGAAAGTGATGAACTTTTCTGTGTGACTGTAAAACCTGCCTGCAACAGCA
  GCAGCTTCCGGCAGCATGGCCCTCTGGTCTGTGATGGCTTCCGGGACTGCGAGGATGGTCAGGACGAGCA
  AAACTGCACTAGAAGCATCCCATGCACCAGTAGGACCTTTAAATGTGGCAATGACATTTGCTTCAGGAAG
  CAAAATGCACAATGTGATGGTATAGTGGACTGTCCAGATGGAAGTGACGAGGAAGGCTGCGGCTGTAGCA
  GGAGTTCTTCCTTCCTTCACCGAATCGTTGGTGGTTCTGACTCCCAGGAGGGGACCTGGCCATGGCAGGT
  CAGCCTCCACTTTGTTGGATCAGCCTACTGTGGTGCCTCAGTCATCTCCAGGGAGTGGCTTCTCTCTGCA
  GCCCATTGTTTCCATGGAAACAGGCTGTCTGACCCCACGCCATGGACTGCTCACCTTGGGATGTATGTGC
  AGGGGAATGCCAAGTTTATCTCTCCAGTGAGAAGGATTGTGGTCCATGAATACTACAACAGCCAGACTTT
  TGACTATGACATTGCCTTGCTGCAGCTCAGCATAGCCTGGCCTGAAACCCTGAAACAGCTCATTCAACCA
  ATATGCATTCCTCCAGCGGGCCAGAAAGTGCGCAGTGGGGAGAAGTGCTGGGTGACTGGCTGGGGGAGAA
  GGCATGAAGCAGATAGTAAGGGCTCCCCGGTTTTGCAGCAAGCGGAGGTAGAGCTCATAGATCAAACCGT
  CTGTGTTTCCACCTATGGGATCATCACGTCGCGCATGCTCTGTGCAGGGGTTATGTCTGGCAAGAGCGAC
  GCCTGCAAAGGAGATTCAGGAGGACCTTTGTCTTGTCGAAGAAAGAGTGATGGAAAATGGATTCTGACTG
  GCATTGTTAGCTGGGGACATGGATGTGGAAGACCCAATTTTCCTGGTGTCTACACAAGGGTGTCAAGCTT
  TGTTCCCTGGATTCATAAGTATGTCCCTTCTCTTTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225414 representing NM_172455
  Red=Cloning site Green=Tags(s)
  
  MDKEKSDPSCKSSDLKISNISIQVVSVPGKLPGRRPPRKPIGKPRPRKQPKKRAPFWNVQNKIILFTVFL
  FILAVTAWTLLWLYISKTESKDAFYFVGMFRITNIEFLPEYRQKESREFLSMAKTVQQVVNLVYTTSAFS
  KFYKQSVVADVSSNNKGGLLVHFWIVFVMPHAKGHIFCEECVAAILKDSIQTSIINRTSVGSLQGLAVDM
  DSVVLNAGLRSDYSSAVGSDNGCSRYLYADHLTLRYPLEISATSGQLMCHFKLVAIVGYLIRLSIESIQL
  EADNCITDSLTVYDSLLPIRSAILYRICEPTRTLMSFVSTNNLMLVILKSPYVRRLAGIRAYFEVIPEQK
  CESTILVKEINSFEGKISSPYYPSYYPPKCKCTWTFQTSLSTLGIALKFYNYSITKKSAKGCEHGWWEIN
  EHMYCGSYMDHETIFRVPSPLVHIQLQCSSRLSDKPLLVEYGGYNISQPCPAGSFRCSSGLCVPQAQRCD
  GVNDCFDESDELFCVTVKPACNSSSFRQHGPLVCDGFRDCEDGQDEQNCTRSIPCTSRTFKCGNDICFRK
  QNAQCDGIVDCPDGSDEEGCGCSRSSSFLHRIVGGSDSQEGTWPWQVSLHFVGSAYCGASVISREWLLSA
  AHCFHGNRLSDPTPWTAHLGMYVQGNAKFISPVRRIVVHEYYNSQTFDYDIALLQLSIAWPETLKQLIQP
  ICIPPAGQKVRSGEKCWVTGWGRRHEADSKGSPVLQQAEVELIDQTVCVSTYGIITSRMLCAGVMSGKSD
  ACKGDSGGPLSCRRKSDGKWILTGIVSWGHGCGRPNFPGVYTRVSSFVPWIHKYVPSLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172455
• ORF Size: 2487 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172455.3, NP_766043.3
• RefSeq Size: 2585 bp
• RefSeq ORF: 2490 bp
• Locus ID: 208171
• UniProt ID: Q8BIK6
• Cytogenetics: 16 B5
• MW: 92.6 kDa
• Gene Summary: Serine protease which preferentially hydrolyzes peptides with Arg at the P1 position.[UniProtKB/Swiss-Prot Function]