Kcnq2 (NM_010611) Mouse Tagged ORF Clone

CAT#: MR225330

Kcnq2 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, subfamily Q, member 2 (Kcnq2), transcript variant 1

Product Images

Circular map for MR225330

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnq2
• Synonyms: HNSPC; KQT2; Nmf134
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225330 representing NM_010611
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGAAGTCGCGCAACGGTGGCGTGTACCCCGGCACCAGCGGGGAAAAGAAGCTCAAGGTGGGCT
  TCGTGGGGCTGGACCCCGGCGCGCCCGACTCCACACGCGACGGCGCGCTACTCATCGCGGGCTCCGAGGC
  CCCCAAGCGCGGCAGCGTTTTGAGCAAGCCGCGGACGGGCGGCGCGGGAGCCGGGAAGCCCCCGAAGCGC
  AACGCCTTCTACCGCAAGCTGCAGAATTTCCTCTACAACGTGCTAGAGCGGCCCCGCGGCTGGGCGTTCA
  TCTACCACGCCTACGTGTTCCTTTTAGTCTTCTCCTGCCTTGTGCTTTCTGTGTTTTCCACCATCAAGGA
  GTACGAGAAGAGCTCTGAGGGGGCCCTCTACATCTTGGAAATCGTGACTATCGTGGTATTCGGTGTTGAG
  TACTTTGTGAGGATCTGGGCTGCAGGCTGCTGTTGCCGGTATCGAGGCTGGAGGGGCAGGCTCAAGTTTG
  CCAGGAAGCCGTTCTGTGTGATTGATATCATGGTGCTGATTGCCTCCATTGCTGTGCTGGCTGCTGGTTC
  CCAGGGCAATGTCTTTGCCACATCTGCGCTTCGGAGCTTGCGGTTCTTGCAAATCTTGCGGATGATCCGT
  ATGGACCGGAGGGGTGGCACCTGGAAGCTCTTGGGATCGGTAGTCTACGCTCACAGCAAGGAGCTGGTGA
  CTGCCTGGTACATTGGCTTCCTCTGCCTCATCCTGGCCTCATTTCTGGTGTACTTGGCAGAAAAGGGTGA
  GAATGACCACTTTGACACCTACGCAGATGCACTCTGGTGGGGTCTGATCACCCTGACGACCATTGGCTAC
  GGGGACAAGTACCCTCAGACCTGGAACGGGAGGCTGCTGGCAGCGACCTTTACCCTCATTGGTGTCTCGT
  TCTTTGCTCTTCCTGCTGGCATTTTGGGATCCGGCTTTGCCCTGAAAGTCCAAGAGCAGCATCGGCAAAA
  ACACTTTGAGAAACGGCGGAACCCTGCGGCAGGTCTGATCCAGTCTGCCTGGAGATTCTATGCTACTAAC
  CTCTCACGCACCGACCTGCACTCCACGTGGCAGTACTACGAGCGGACAGTCACTGTCCCCATGTACAGCT
  CACAAACTCAAACCTATGGGGCCTCCAGACTCATCCCACCTCTGAACCAGCTGGAGCTGCTGAGGAATCT
  CAAGAGCAAATCTGGACTCACCTTCAGGAAGGAGCCACAGCCAGAGCCATCACCAAGTAAAGGCAGACCG
  TGCCGAGGGTGCCTGTGTGGATGCTGCCCCGGACACTCTAGTCAGAAGGTCAGTTTGAAAGATCGTGTCT
  TCTCCAGCCCCCGAGGCATGGCTGCCAAGGGAAAGGGGTCTCCCCAGGCCCAGACGGTCCGGCGGTCCCC
  CAGTGCGGATCAGAGTCTTGATGACAGCCCGAGCAAGGTGCCCAAGAGCTGGAGCTTTGGTGACCGCAGC
  CGCACACGCCAGGCTTTCCGCATCAAGGGTGCTGCATCCCGGCAGAATTCAGAAGAAGCAAGCCTCCCTG
  GGGAGGACATCGTAGAGGACAACAAGAGCTGTAACTGCGAGTTTGTGACTGAAGATCTTACCCCTGGCCT
  CAAAGTTAGCATCAGAGCTGTGTGTGTTATGCGGTTCTTGGTATCTAAGCGAAAGTTCAAAGAGAGTCTG
  CGCCCATATGATGTGATGGACGTCATCGAACAGTACTCGGCTGGACACTTGGATATGTTGTCCCGCATCA
  AGAGCCTGCAGTCCAGAGTGGACCAGATTGTGGGGCGGGGCCCAACAATAACGGATAAGGACCGCACCAA
  AGGCCCAGCGGAAACGGAGCTGCCCGAAGACCCCAGCATGATGGGACGGCTTGGGAAGGTGGAGAAACAG
  GTCTTGTCCATGGAAAAGAAGCTCGACTTCTTGGTGAGCATCTATACACAGAGAATGGGCATCCCACCAG
  CAGAGACAGAGGCCTATTTTGGGGCCAAGGAGCCTGAGCCGGCACCACCCTACCACAGCCCAGAGGACAG
  CCGTGACCATGCAGACAAGCATGGCTGTATCATTAAGATCGTCCGCTCCACCAGCTCTACGGGCCAGAGG
  AACTACGCAGCACCCCCAGCCATCCCCCCTGCCCAGTGTCCTCCCTCCACCTCGTGGCAGCAGAGCCACC
  AGCGCCATGGCACCTCCCCTGTGGGAGACCATGGCTCACTGGTACGCATCCCACCACCCCCTGCACACGA
  GCGGTCGCTGTCTGCCTACGGTGGGGGCAACAGAGCCAGTACCGAGTTCTTGAGGCTGGAGGGCACCCCA
  GCCTGCAGGCCCTCTGAGGCTGCCCTGCGGGATAGCGACACGTCCATCTCCATCCCTTCGGTGGACCATG
  AGGAGCTGGAGCGCTCCTTCAGCGGCTTCAGTATCTCCCAGTCCAAGGAGAACCTGGATGCCCTGGGCAG
  CTGTTATGCAGCTGTGGCACCATGCGCCAAGGTCAGGCCCTACATTGCAGAGGGAGAGTCTGACACAGAC
  TCAGACCTCTGCACACCATGTGGGCCTCCCCCACGCTCTGCCACTGGTGAGGGCCCCTTTGGAGATGTGG
  CTTGGGCAGGGCCCAGGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225330 representing NM_010611
  Red=Cloning site Green=Tags(s)
  
  MVQKSRNGGVYPGTSGEKKLKVGFVGLDPGAPDSTRDGALLIAGSEAPKRGSVLSKPRTGGAGAGKPPKR
  NAFYRKLQNFLYNVLERPRGWAFIYHAYVFLLVFSCLVLSVFSTIKEYEKSSEGALYILEIVTIVVFGVE
  YFVRIWAAGCCCRYRGWRGRLKFARKPFCVIDIMVLIASIAVLAAGSQGNVFATSALRSLRFLQILRMIR
  MDRRGGTWKLLGSVVYAHSKELVTAWYIGFLCLILASFLVYLAEKGENDHFDTYADALWWGLITLTTIGY
  GDKYPQTWNGRLLAATFTLIGVSFFALPAGILGSGFALKVQEQHRQKHFEKRRNPAAGLIQSAWRFYATN
  LSRTDLHSTWQYYERTVTVPMYSSQTQTYGASRLIPPLNQLELLRNLKSKSGLTFRKEPQPEPSPSKGRP
  CRGCLCGCCPGHSSQKVSLKDRVFSSPRGMAAKGKGSPQAQTVRRSPSADQSLDDSPSKVPKSWSFGDRS
  RTRQAFRIKGAASRQNSEEASLPGEDIVEDNKSCNCEFVTEDLTPGLKVSIRAVCVMRFLVSKRKFKESL
  RPYDVMDVIEQYSAGHLDMLSRIKSLQSRVDQIVGRGPTITDKDRTKGPAETELPEDPSMMGRLGKVEKQ
  VLSMEKKLDFLVSIYTQRMGIPPAETEAYFGAKEPEPAPPYHSPEDSRDHADKHGCIIKIVRSTSSTGQR
  NYAAPPAIPPAQCPPSTSWQQSHQRHGTSPVGDHGSLVRIPPPPAHERSLSAYGGGNRASTEFLRLEGTP
  ACRPSEAALRDSDTSISIPSVDHEELERSFSGFSISQSKENLDALGSCYAAVAPCAKVRPYIAEGESDTD
  SDLCTPCGPPPRSATGEGPFGDVAWAGPRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010611
• ORF Size: 2610 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010611.3, NP_034741.2
• RefSeq Size: 2777 bp
• RefSeq ORF: 2613 bp
• Locus ID: 16536
• UniProt ID: Q9Z351
• Cytogenetics: 2 103.57 cM
• MW: 96.2 kDa
• Gene Summary: Associates with KCNQ3 to form a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. Therefore, it is important in the regulation of neuronal excitability.[UniProtKB/Swiss-Prot Function]